Radiation therapy induces the DNA damage response in peripheral blood

Stereotactic body radiation therapy (SBRT) is a radiotherapy modality that delivers highly conformal, ablative doses to a well-defined target. Here, using a semiquantitative multiplexed assay to analyze ATM and H2AX phosphorylation, we show that ATM kinase activity in peripheral blood mononuclear ce...

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Detalles Bibliográficos
Autores principales: Bakkenist, Christopher J., Czambel, R. Kenneth, Clump, David A., Greenberger, Joel S., Beumer, Jan H., Schmitz, John C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2013
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3787146/
https://www.ncbi.nlm.nih.gov/pubmed/23900392
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author Bakkenist, Christopher J.
Czambel, R. Kenneth
Clump, David A.
Greenberger, Joel S.
Beumer, Jan H.
Schmitz, John C.
author_facet Bakkenist, Christopher J.
Czambel, R. Kenneth
Clump, David A.
Greenberger, Joel S.
Beumer, Jan H.
Schmitz, John C.
author_sort Bakkenist, Christopher J.
collection PubMed
description Stereotactic body radiation therapy (SBRT) is a radiotherapy modality that delivers highly conformal, ablative doses to a well-defined target. Here, using a semiquantitative multiplexed assay to analyze ATM and H2AX phosphorylation, we show that ATM kinase activity in peripheral blood mononuclear cells is induced following SBRT. This observation of a systemic ATM kinase-dependent DNA damage response in the peripheral blood is unprecedented and promotes the use of ATM serine-1981 phosphorylation as a predictive biomarker for DNA damaging modalities and ATM inhibitors.
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spelling pubmed-37871462013-10-01 Radiation therapy induces the DNA damage response in peripheral blood Bakkenist, Christopher J. Czambel, R. Kenneth Clump, David A. Greenberger, Joel S. Beumer, Jan H. Schmitz, John C. Oncotarget Research Paper Stereotactic body radiation therapy (SBRT) is a radiotherapy modality that delivers highly conformal, ablative doses to a well-defined target. Here, using a semiquantitative multiplexed assay to analyze ATM and H2AX phosphorylation, we show that ATM kinase activity in peripheral blood mononuclear cells is induced following SBRT. This observation of a systemic ATM kinase-dependent DNA damage response in the peripheral blood is unprecedented and promotes the use of ATM serine-1981 phosphorylation as a predictive biomarker for DNA damaging modalities and ATM inhibitors. Impact Journals LLC 2013-06-26 /pmc/articles/PMC3787146/ /pubmed/23900392 Text en Copyright: © 2013 Bakkenist et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
spellingShingle Research Paper
Bakkenist, Christopher J.
Czambel, R. Kenneth
Clump, David A.
Greenberger, Joel S.
Beumer, Jan H.
Schmitz, John C.
Radiation therapy induces the DNA damage response in peripheral blood
title Radiation therapy induces the DNA damage response in peripheral blood
title_full Radiation therapy induces the DNA damage response in peripheral blood
title_fullStr Radiation therapy induces the DNA damage response in peripheral blood
title_full_unstemmed Radiation therapy induces the DNA damage response in peripheral blood
title_short Radiation therapy induces the DNA damage response in peripheral blood
title_sort radiation therapy induces the dna damage response in peripheral blood
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3787146/
https://www.ncbi.nlm.nih.gov/pubmed/23900392
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