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A method for the three-dimensional reconstruction of Neurobiotin™-filled neurons and the location of their synaptic inputs
Here, we describe a robust method for mapping the number and type of neuro-chemically distinct synaptic inputs that a single reconstructed neuron receives. We have used individual hypoglossal motor neurons filled with Neurobiotin by semi-loose seal electroporation in thick brainstem slices. These fi...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Frontiers Media S.A.
2013
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3787200/ https://www.ncbi.nlm.nih.gov/pubmed/24101895 http://dx.doi.org/10.3389/fncir.2013.00153 |