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Novel Spectrophotometric Method for the Quantitation of Urinary Xanthurenic Acid and Its Application in Identifying Individuals with Hyperhomocysteinemia Associated with Vitamin B(6) Deficiency
A novel spectrophotometric method for the quantification of urinary xanthurenic acid (XA) is described. The direct acid ferric reduction (DAFR) procedure was used to quantify XA after it was purified by a solid-phase extraction column. The linearity of proposed method extends from 2.5 to 100.0 mg/L....
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3787575/ https://www.ncbi.nlm.nih.gov/pubmed/24151616 http://dx.doi.org/10.1155/2013/678476 |
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author | Chen, Chi-Fen Liu, Tsan-Zon Lan, Wu-Hsiang Wu, Li-An Tsai, Chin-Hung Chiou, Jeng-Fong Tsai, Li-Yu |
author_facet | Chen, Chi-Fen Liu, Tsan-Zon Lan, Wu-Hsiang Wu, Li-An Tsai, Chin-Hung Chiou, Jeng-Fong Tsai, Li-Yu |
author_sort | Chen, Chi-Fen |
collection | PubMed |
description | A novel spectrophotometric method for the quantification of urinary xanthurenic acid (XA) is described. The direct acid ferric reduction (DAFR) procedure was used to quantify XA after it was purified by a solid-phase extraction column. The linearity of proposed method extends from 2.5 to 100.0 mg/L. The method is precise, yielding day-to-day CVs for two pooled controls of 3.5% and 4.6%, respectively. Correlation studies with an established HPLC method and a fluorometric procedure showed correlation coefficients of 0.98 and 0.98, respectively. Interference from various urinary metabolites was insignificant. In a small-scale screening of elderly conducted at Penghu county in Taiwan (n = 80), we were able to identify a group of twenty individuals having hyperhomocysteinemia (>15 μmole/L). Three of them were found to be positive for XA as analyzed by the proposed method, which correlated excellently with the results of the activation coefficient method for RBC's AST/B(6) functional test. These data confirm the usefulness of the proposed method for identifying urinary XA as an indicator of vitamin B(6) deficiency-associated hyperhomocysteinemic condition. |
format | Online Article Text |
id | pubmed-3787575 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-37875752013-10-22 Novel Spectrophotometric Method for the Quantitation of Urinary Xanthurenic Acid and Its Application in Identifying Individuals with Hyperhomocysteinemia Associated with Vitamin B(6) Deficiency Chen, Chi-Fen Liu, Tsan-Zon Lan, Wu-Hsiang Wu, Li-An Tsai, Chin-Hung Chiou, Jeng-Fong Tsai, Li-Yu Biomed Res Int Research Article A novel spectrophotometric method for the quantification of urinary xanthurenic acid (XA) is described. The direct acid ferric reduction (DAFR) procedure was used to quantify XA after it was purified by a solid-phase extraction column. The linearity of proposed method extends from 2.5 to 100.0 mg/L. The method is precise, yielding day-to-day CVs for two pooled controls of 3.5% and 4.6%, respectively. Correlation studies with an established HPLC method and a fluorometric procedure showed correlation coefficients of 0.98 and 0.98, respectively. Interference from various urinary metabolites was insignificant. In a small-scale screening of elderly conducted at Penghu county in Taiwan (n = 80), we were able to identify a group of twenty individuals having hyperhomocysteinemia (>15 μmole/L). Three of them were found to be positive for XA as analyzed by the proposed method, which correlated excellently with the results of the activation coefficient method for RBC's AST/B(6) functional test. These data confirm the usefulness of the proposed method for identifying urinary XA as an indicator of vitamin B(6) deficiency-associated hyperhomocysteinemic condition. Hindawi Publishing Corporation 2013 2013-09-16 /pmc/articles/PMC3787575/ /pubmed/24151616 http://dx.doi.org/10.1155/2013/678476 Text en Copyright © 2013 Chi-Fen Chen et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Chen, Chi-Fen Liu, Tsan-Zon Lan, Wu-Hsiang Wu, Li-An Tsai, Chin-Hung Chiou, Jeng-Fong Tsai, Li-Yu Novel Spectrophotometric Method for the Quantitation of Urinary Xanthurenic Acid and Its Application in Identifying Individuals with Hyperhomocysteinemia Associated with Vitamin B(6) Deficiency |
title | Novel Spectrophotometric Method for the Quantitation of Urinary Xanthurenic Acid and Its Application in Identifying Individuals with Hyperhomocysteinemia Associated with Vitamin B(6) Deficiency |
title_full | Novel Spectrophotometric Method for the Quantitation of Urinary Xanthurenic Acid and Its Application in Identifying Individuals with Hyperhomocysteinemia Associated with Vitamin B(6) Deficiency |
title_fullStr | Novel Spectrophotometric Method for the Quantitation of Urinary Xanthurenic Acid and Its Application in Identifying Individuals with Hyperhomocysteinemia Associated with Vitamin B(6) Deficiency |
title_full_unstemmed | Novel Spectrophotometric Method for the Quantitation of Urinary Xanthurenic Acid and Its Application in Identifying Individuals with Hyperhomocysteinemia Associated with Vitamin B(6) Deficiency |
title_short | Novel Spectrophotometric Method for the Quantitation of Urinary Xanthurenic Acid and Its Application in Identifying Individuals with Hyperhomocysteinemia Associated with Vitamin B(6) Deficiency |
title_sort | novel spectrophotometric method for the quantitation of urinary xanthurenic acid and its application in identifying individuals with hyperhomocysteinemia associated with vitamin b(6) deficiency |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3787575/ https://www.ncbi.nlm.nih.gov/pubmed/24151616 http://dx.doi.org/10.1155/2013/678476 |
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