A Sensitive Chemotaxis Assay Using a Novel Microfluidic Device

Existing chemotaxis assays do not generate stable chemotactic gradients and thus—over time—functionally measure only nonspecific random motion (chemokinesis). In comparison, microfluidic technology has the capacity to generate a tightly controlled microenvironment that can be stably maintained for e...

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Detalles Bibliográficos
Autores principales: Zhang, Chen, Jang, Sunyoung, Amadi, Ovid C., Shimizu, Koichi, Lee, Richard T., Mitchell, Richard N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Clinical Study
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3787577/
https://www.ncbi.nlm.nih.gov/pubmed/24151597
http://dx.doi.org/10.1155/2013/373569
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author Zhang, Chen
Jang, Sunyoung
Amadi, Ovid C.
Shimizu, Koichi
Lee, Richard T.
Mitchell, Richard N.
author_facet Zhang, Chen
Jang, Sunyoung
Amadi, Ovid C.
Shimizu, Koichi
Lee, Richard T.
Mitchell, Richard N.
author_sort Zhang, Chen
collection PubMed
description Existing chemotaxis assays do not generate stable chemotactic gradients and thus—over time—functionally measure only nonspecific random motion (chemokinesis). In comparison, microfluidic technology has the capacity to generate a tightly controlled microenvironment that can be stably maintained for extended periods of time and is, therefore, amenable to adaptation for assaying chemotaxis. We describe here a novel microfluidic device for sensitive assay of cellular migration and show its application for evaluating the chemotaxis of smooth muscle cells in a chemokine gradient.
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spelling pubmed-37875772013-10-22 A Sensitive Chemotaxis Assay Using a Novel Microfluidic Device Zhang, Chen Jang, Sunyoung Amadi, Ovid C. Shimizu, Koichi Lee, Richard T. Mitchell, Richard N. Biomed Res Int Clinical Study Existing chemotaxis assays do not generate stable chemotactic gradients and thus—over time—functionally measure only nonspecific random motion (chemokinesis). In comparison, microfluidic technology has the capacity to generate a tightly controlled microenvironment that can be stably maintained for extended periods of time and is, therefore, amenable to adaptation for assaying chemotaxis. We describe here a novel microfluidic device for sensitive assay of cellular migration and show its application for evaluating the chemotaxis of smooth muscle cells in a chemokine gradient. Hindawi Publishing Corporation 2013 2013-09-14 /pmc/articles/PMC3787577/ /pubmed/24151597 http://dx.doi.org/10.1155/2013/373569 Text en Copyright © 2013 Chen Zhang et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Clinical Study
Zhang, Chen
Jang, Sunyoung
Amadi, Ovid C.
Shimizu, Koichi
Lee, Richard T.
Mitchell, Richard N.
A Sensitive Chemotaxis Assay Using a Novel Microfluidic Device
title A Sensitive Chemotaxis Assay Using a Novel Microfluidic Device
title_full A Sensitive Chemotaxis Assay Using a Novel Microfluidic Device
title_fullStr A Sensitive Chemotaxis Assay Using a Novel Microfluidic Device
title_full_unstemmed A Sensitive Chemotaxis Assay Using a Novel Microfluidic Device
title_short A Sensitive Chemotaxis Assay Using a Novel Microfluidic Device
title_sort sensitive chemotaxis assay using a novel microfluidic device
topic Clinical Study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3787577/
https://www.ncbi.nlm.nih.gov/pubmed/24151597
http://dx.doi.org/10.1155/2013/373569
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