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GC/MS analysis of high-performance liquid chromatography fractions from Sophora flavescens and Torilis japonica extracts and their in vitro anti-neosporal effects on Neospora caninum

We analyzed alcoholic extracts of herbs possessing anti-neosporal activity against Neospora (N.) caninum. To identify the chemical components of Sophora (S.) flavescens and Torilis (T.) japonica associated with anti-neosporal activity, specific fractions were isolated by high-performance liquid chro...

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Autores principales: Seo, Hun-Su, Kim, Kyoung Hee, Kim, Dae-Yong, Park, Bong-Kyun, Shin, Nam-Shik, Kim, Jae-Hoon, Youn, Heejeong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Veterinary Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3788148/
https://www.ncbi.nlm.nih.gov/pubmed/23820198
http://dx.doi.org/10.4142/jvs.2013.14.3.241
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author Seo, Hun-Su
Kim, Kyoung Hee
Kim, Dae-Yong
Park, Bong-Kyun
Shin, Nam-Shik
Kim, Jae-Hoon
Youn, Heejeong
author_facet Seo, Hun-Su
Kim, Kyoung Hee
Kim, Dae-Yong
Park, Bong-Kyun
Shin, Nam-Shik
Kim, Jae-Hoon
Youn, Heejeong
author_sort Seo, Hun-Su
collection PubMed
description We analyzed alcoholic extracts of herbs possessing anti-neosporal activity against Neospora (N.) caninum. To identify the chemical components of Sophora (S.) flavescens and Torilis (T.) japonica associated with anti-neosporal activity, specific fractions were isolated by high-performance liquid chromatography (HPLC). In vitro activity of the fractions against N. caninum was then assessed. Gas chromatography/mass spectrometry (GC/MS) was used to identify and quantify specific anti-neosporal molecules in the herbal extracts. Almost all HPLC fractions of S. flavescens and T. japonica had higher levels of anti-neosporal activity compared to the not treated control. Active constituents of the extracts were sophoridane, furosardonin A, and tetraisopropylidene-cyclobutane in S. flavescens; 5,17-β-dihydroxy-de-A-estra-5,7,9,14-tetraene, furanodiene, and 9,12-octadecadienoic acid (Z,Z)-(CAS,1) in T. japonica.
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spelling pubmed-37881482013-10-04 GC/MS analysis of high-performance liquid chromatography fractions from Sophora flavescens and Torilis japonica extracts and their in vitro anti-neosporal effects on Neospora caninum Seo, Hun-Su Kim, Kyoung Hee Kim, Dae-Yong Park, Bong-Kyun Shin, Nam-Shik Kim, Jae-Hoon Youn, Heejeong J Vet Sci Original Article We analyzed alcoholic extracts of herbs possessing anti-neosporal activity against Neospora (N.) caninum. To identify the chemical components of Sophora (S.) flavescens and Torilis (T.) japonica associated with anti-neosporal activity, specific fractions were isolated by high-performance liquid chromatography (HPLC). In vitro activity of the fractions against N. caninum was then assessed. Gas chromatography/mass spectrometry (GC/MS) was used to identify and quantify specific anti-neosporal molecules in the herbal extracts. Almost all HPLC fractions of S. flavescens and T. japonica had higher levels of anti-neosporal activity compared to the not treated control. Active constituents of the extracts were sophoridane, furosardonin A, and tetraisopropylidene-cyclobutane in S. flavescens; 5,17-β-dihydroxy-de-A-estra-5,7,9,14-tetraene, furanodiene, and 9,12-octadecadienoic acid (Z,Z)-(CAS,1) in T. japonica. The Korean Society of Veterinary Science 2013-09 2013-09-25 /pmc/articles/PMC3788148/ /pubmed/23820198 http://dx.doi.org/10.4142/jvs.2013.14.3.241 Text en © 2013 The Korean Society of Veterinary Science. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Seo, Hun-Su
Kim, Kyoung Hee
Kim, Dae-Yong
Park, Bong-Kyun
Shin, Nam-Shik
Kim, Jae-Hoon
Youn, Heejeong
GC/MS analysis of high-performance liquid chromatography fractions from Sophora flavescens and Torilis japonica extracts and their in vitro anti-neosporal effects on Neospora caninum
title GC/MS analysis of high-performance liquid chromatography fractions from Sophora flavescens and Torilis japonica extracts and their in vitro anti-neosporal effects on Neospora caninum
title_full GC/MS analysis of high-performance liquid chromatography fractions from Sophora flavescens and Torilis japonica extracts and their in vitro anti-neosporal effects on Neospora caninum
title_fullStr GC/MS analysis of high-performance liquid chromatography fractions from Sophora flavescens and Torilis japonica extracts and their in vitro anti-neosporal effects on Neospora caninum
title_full_unstemmed GC/MS analysis of high-performance liquid chromatography fractions from Sophora flavescens and Torilis japonica extracts and their in vitro anti-neosporal effects on Neospora caninum
title_short GC/MS analysis of high-performance liquid chromatography fractions from Sophora flavescens and Torilis japonica extracts and their in vitro anti-neosporal effects on Neospora caninum
title_sort gc/ms analysis of high-performance liquid chromatography fractions from sophora flavescens and torilis japonica extracts and their in vitro anti-neosporal effects on neospora caninum
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3788148/
https://www.ncbi.nlm.nih.gov/pubmed/23820198
http://dx.doi.org/10.4142/jvs.2013.14.3.241
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