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Temporal changes of Japanese encephalitits virus in different brain regions of rat

BACKGROUND & OBJECTIVES: Japanese encephalitis virus (JEV) infection results in acute encephalitic illness. The affinity of JEV to different regions of brain and temporal changes in viral load have not been studied. This study was conducted to describe localization of JEV to different regions of...

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Autores principales: Srivastava, Ruchi, Kalita, Jayantee, Khan, Mohammad Y., Gore, Milind M., Bondre, Vijay P., Misra, Usha K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3788207/
https://www.ncbi.nlm.nih.gov/pubmed/24056598
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author Srivastava, Ruchi
Kalita, Jayantee
Khan, Mohammad Y.
Gore, Milind M.
Bondre, Vijay P.
Misra, Usha K.
author_facet Srivastava, Ruchi
Kalita, Jayantee
Khan, Mohammad Y.
Gore, Milind M.
Bondre, Vijay P.
Misra, Usha K.
author_sort Srivastava, Ruchi
collection PubMed
description BACKGROUND & OBJECTIVES: Japanese encephalitis virus (JEV) infection results in acute encephalitic illness. The affinity of JEV to different regions of brain and temporal changes in viral load have not been studied. This study was conducted to describe localization of JEV to different regions of the brain at different stages of disease in a rat model of Japanese encephalitis (JE). METHODS: Twelve days old Wistar rats were inoculated intracerebrally with a dose of 3 × 10(6) pfu/ml of JEV. After 3, 6, 10 and 20 days post-inoculation, brains were dissected out and different regions of brain (cortex, striatum, thalamus and mid brain) were taken. Motor deficit was assessed by the rota rod and JEV RNA copies were evaluated using real-time PCR assay. RESULTS: There was a significant increase in motor deficit in rats inoculated with JEV compared to the controls. JEV RNA copies were present in all studied regions of the brain on days 3, 6 and 10 post-inoculation. Maximum number of JEV RNA copies were present in the mid brain on days 3 and 10 post-inoculation. JEV RNA copies were not detected in any of the brain regions on day 20. INTERPRETATION & CONCLUSIONS: This study reports JEV RNA load in different brain regions of rat with higher affinity of JEV virus to thalamus and mid brain compared to other regions.
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spelling pubmed-37882072013-10-04 Temporal changes of Japanese encephalitits virus in different brain regions of rat Srivastava, Ruchi Kalita, Jayantee Khan, Mohammad Y. Gore, Milind M. Bondre, Vijay P. Misra, Usha K. Indian J Med Res Original Article BACKGROUND & OBJECTIVES: Japanese encephalitis virus (JEV) infection results in acute encephalitic illness. The affinity of JEV to different regions of brain and temporal changes in viral load have not been studied. This study was conducted to describe localization of JEV to different regions of the brain at different stages of disease in a rat model of Japanese encephalitis (JE). METHODS: Twelve days old Wistar rats were inoculated intracerebrally with a dose of 3 × 10(6) pfu/ml of JEV. After 3, 6, 10 and 20 days post-inoculation, brains were dissected out and different regions of brain (cortex, striatum, thalamus and mid brain) were taken. Motor deficit was assessed by the rota rod and JEV RNA copies were evaluated using real-time PCR assay. RESULTS: There was a significant increase in motor deficit in rats inoculated with JEV compared to the controls. JEV RNA copies were present in all studied regions of the brain on days 3, 6 and 10 post-inoculation. Maximum number of JEV RNA copies were present in the mid brain on days 3 and 10 post-inoculation. JEV RNA copies were not detected in any of the brain regions on day 20. INTERPRETATION & CONCLUSIONS: This study reports JEV RNA load in different brain regions of rat with higher affinity of JEV virus to thalamus and mid brain compared to other regions. Medknow Publications & Media Pvt Ltd 2013-08 /pmc/articles/PMC3788207/ /pubmed/24056598 Text en Copyright: © The Indian Journal of Medical Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Srivastava, Ruchi
Kalita, Jayantee
Khan, Mohammad Y.
Gore, Milind M.
Bondre, Vijay P.
Misra, Usha K.
Temporal changes of Japanese encephalitits virus in different brain regions of rat
title Temporal changes of Japanese encephalitits virus in different brain regions of rat
title_full Temporal changes of Japanese encephalitits virus in different brain regions of rat
title_fullStr Temporal changes of Japanese encephalitits virus in different brain regions of rat
title_full_unstemmed Temporal changes of Japanese encephalitits virus in different brain regions of rat
title_short Temporal changes of Japanese encephalitits virus in different brain regions of rat
title_sort temporal changes of japanese encephalitits virus in different brain regions of rat
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3788207/
https://www.ncbi.nlm.nih.gov/pubmed/24056598
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