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Roles of mitochondrial transcription factor A and microRNA-590-3p in the development of bladder cancer

Mitochondrial transcription factor A (TFAM) is required for mitochondrial DNA (mtDNA) replication and transcription. microRNAs (miRNAs) act as key factors in the regulation of gene expression. However, the roles of TFAM and certain miRNAs and their association in cancer development remain unclear. T...

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Autores principales: MO, MIAO, PENG, FENGHUA, WANG, LU, PENG, LONGKAI, LAN, GONGBIN, YU, SHAOJIE
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3789041/
https://www.ncbi.nlm.nih.gov/pubmed/24137381
http://dx.doi.org/10.3892/ol.2013.1419
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author MO, MIAO
PENG, FENGHUA
WANG, LU
PENG, LONGKAI
LAN, GONGBIN
YU, SHAOJIE
author_facet MO, MIAO
PENG, FENGHUA
WANG, LU
PENG, LONGKAI
LAN, GONGBIN
YU, SHAOJIE
author_sort MO, MIAO
collection PubMed
description Mitochondrial transcription factor A (TFAM) is required for mitochondrial DNA (mtDNA) replication and transcription. microRNAs (miRNAs) act as key factors in the regulation of gene expression. However, the roles of TFAM and certain miRNAs and their association in cancer development remain unclear. The present study reported that the expression of TFAM was significantly increased in bladder cancer, while the expression of miRNA-590-3p was downregulated. The luciferase assay showed that TFAM was the direct target of miRNA-590-3p. Furthermore, the forced overexpression of miRNA-590-3p significantly inhibited the proliferation, migration and colony-forming ability of 5637 cells, which was in contrast with the results from the forced overexpression of TFAM in the 5637 cells. Furthermore, cell proliferation- and migration-related genes, including phosphoinositide-3-kinase (PI3K), Akt, matrix metalloproteinase (MMP)-2 and MMP9, were significantly downregulated in the miRNA-590-3p-overex-pressing 5637 cells, but upregulated in the TFAM-overexpressing cells. In conclusion, the present study suggested that TFAM, a direct target of miRNA-590-3p, may play a significant role in the tumorigenesis of bladder cancer and thus may be a promising target for cancer therapeutics.
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spelling pubmed-37890412013-10-17 Roles of mitochondrial transcription factor A and microRNA-590-3p in the development of bladder cancer MO, MIAO PENG, FENGHUA WANG, LU PENG, LONGKAI LAN, GONGBIN YU, SHAOJIE Oncol Lett Articles Mitochondrial transcription factor A (TFAM) is required for mitochondrial DNA (mtDNA) replication and transcription. microRNAs (miRNAs) act as key factors in the regulation of gene expression. However, the roles of TFAM and certain miRNAs and their association in cancer development remain unclear. The present study reported that the expression of TFAM was significantly increased in bladder cancer, while the expression of miRNA-590-3p was downregulated. The luciferase assay showed that TFAM was the direct target of miRNA-590-3p. Furthermore, the forced overexpression of miRNA-590-3p significantly inhibited the proliferation, migration and colony-forming ability of 5637 cells, which was in contrast with the results from the forced overexpression of TFAM in the 5637 cells. Furthermore, cell proliferation- and migration-related genes, including phosphoinositide-3-kinase (PI3K), Akt, matrix metalloproteinase (MMP)-2 and MMP9, were significantly downregulated in the miRNA-590-3p-overex-pressing 5637 cells, but upregulated in the TFAM-overexpressing cells. In conclusion, the present study suggested that TFAM, a direct target of miRNA-590-3p, may play a significant role in the tumorigenesis of bladder cancer and thus may be a promising target for cancer therapeutics. D.A. Spandidos 2013-08 2013-06-21 /pmc/articles/PMC3789041/ /pubmed/24137381 http://dx.doi.org/10.3892/ol.2013.1419 Text en Copyright © 2013, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
MO, MIAO
PENG, FENGHUA
WANG, LU
PENG, LONGKAI
LAN, GONGBIN
YU, SHAOJIE
Roles of mitochondrial transcription factor A and microRNA-590-3p in the development of bladder cancer
title Roles of mitochondrial transcription factor A and microRNA-590-3p in the development of bladder cancer
title_full Roles of mitochondrial transcription factor A and microRNA-590-3p in the development of bladder cancer
title_fullStr Roles of mitochondrial transcription factor A and microRNA-590-3p in the development of bladder cancer
title_full_unstemmed Roles of mitochondrial transcription factor A and microRNA-590-3p in the development of bladder cancer
title_short Roles of mitochondrial transcription factor A and microRNA-590-3p in the development of bladder cancer
title_sort roles of mitochondrial transcription factor a and microrna-590-3p in the development of bladder cancer
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3789041/
https://www.ncbi.nlm.nih.gov/pubmed/24137381
http://dx.doi.org/10.3892/ol.2013.1419
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