Allele-specific detection of single mRNA molecules in situ

We describe a method for fluorescent in situ identification of individual mRNA molecules, allowing quantitative and accurate measurements of allele-specific transcripts that differ by only a few nucleotides, in single cells. By using a combination of allele-specific and non-allele-specific probe lib...

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Detalles Bibliográficos
Autores principales: Hansen, Clinton H., van Oudenaarden, Alexander
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3789122/
https://www.ncbi.nlm.nih.gov/pubmed/23934076
http://dx.doi.org/10.1038/nmeth.2601
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author Hansen, Clinton H.
van Oudenaarden, Alexander
author_facet Hansen, Clinton H.
van Oudenaarden, Alexander
author_sort Hansen, Clinton H.
collection PubMed
description We describe a method for fluorescent in situ identification of individual mRNA molecules, allowing quantitative and accurate measurements of allele-specific transcripts that differ by only a few nucleotides, in single cells. By using a combination of allele-specific and non-allele-specific probe libraries, we achieve over 95% detection accuracy. We investigate the allele-specific stochastic expression of the pluripotency factor Nanog in murine embryonic stem cells.
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spelling pubmed-37891222014-03-01 Allele-specific detection of single mRNA molecules in situ Hansen, Clinton H. van Oudenaarden, Alexander Nat Methods Article We describe a method for fluorescent in situ identification of individual mRNA molecules, allowing quantitative and accurate measurements of allele-specific transcripts that differ by only a few nucleotides, in single cells. By using a combination of allele-specific and non-allele-specific probe libraries, we achieve over 95% detection accuracy. We investigate the allele-specific stochastic expression of the pluripotency factor Nanog in murine embryonic stem cells. 2013-08-11 2013-09 /pmc/articles/PMC3789122/ /pubmed/23934076 http://dx.doi.org/10.1038/nmeth.2601 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Hansen, Clinton H.
van Oudenaarden, Alexander
Allele-specific detection of single mRNA molecules in situ
title Allele-specific detection of single mRNA molecules in situ
title_full Allele-specific detection of single mRNA molecules in situ
title_fullStr Allele-specific detection of single mRNA molecules in situ
title_full_unstemmed Allele-specific detection of single mRNA molecules in situ
title_short Allele-specific detection of single mRNA molecules in situ
title_sort allele-specific detection of single mrna molecules in situ
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3789122/
https://www.ncbi.nlm.nih.gov/pubmed/23934076
http://dx.doi.org/10.1038/nmeth.2601
work_keys_str_mv AT hansenclintonh allelespecificdetectionofsinglemrnamoleculesinsitu
AT vanoudenaardenalexander allelespecificdetectionofsinglemrnamoleculesinsitu

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