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Allele-specific detection of single mRNA molecules in situ
We describe a method for fluorescent in situ identification of individual mRNA molecules, allowing quantitative and accurate measurements of allele-specific transcripts that differ by only a few nucleotides, in single cells. By using a combination of allele-specific and non-allele-specific probe lib...
Autores principales: | Hansen, Clinton H., van Oudenaarden, Alexander |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3789122/ https://www.ncbi.nlm.nih.gov/pubmed/23934076 http://dx.doi.org/10.1038/nmeth.2601 |
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