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Antiherpetic Effects of Gynura procumbens

The ethanol extract of Gynura procumbens showed virucidal and antireplicative actions against herpes simplex virus HSV-1 and HSV-2. It was further chromatographed on MCI gel CHP20P column giving the extract fractions F1 (water), F2 (water-methanol) F3 (methanol), and F4 (ethyl acetate). All but F1 h...

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Detalles Bibliográficos
Autores principales: Jarikasem, Siripen, Charuwichitratana, Somyot, Siritantikorn, Sontana, Chantratita, Wasan, Iskander, Magdy, Frahm, August Wilhelm, Jiratchariyakul, Weena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3789483/
https://www.ncbi.nlm.nih.gov/pubmed/24159345
http://dx.doi.org/10.1155/2013/394865
Descripción
Sumario:The ethanol extract of Gynura procumbens showed virucidal and antireplicative actions against herpes simplex virus HSV-1 and HSV-2. It was further chromatographed on MCI gel CHP20P column giving the extract fractions F1 (water), F2 (water-methanol) F3 (methanol), and F4 (ethyl acetate). All but F1 had virucidal action against both viral types. We reported here the active compounds from F2 and F3. The antiherpetic compounds of F2 was a mixture of dicaffeoylquinic acids with virucidal and antireplicative actions against HSV-2 (IC(50) 96.0 and 61.0 μg/mL, resp.) Virucidal compounds of F3 were a mixture of β-sitosterol and stigmasterol (IC(50) 250.0 μg/mL against HSV-1), a mixture of β-sitosteryl and stigmasteryl glucosides (IC(50) 50.0 μg/mL against HSV-2) and 1, 2-bis-dodecanoyl-3-α-D-glucopyranosyl-sn-glycerol (IC(50) of 40.0 μg/mL against HSV-2). Herbal products containing 1 and 2% of standardized ethanol extract were prepared. Double-blind randomized controlled clinical trial of the products was performed in patients with recurrent herpes labialis. Results showed that the number of patients, whose lesions healed within 7 days and the average healing time of both groups differed insignificantly. Viral culture on D7 indicated a decrease of infected patients from 48.7% to 7.69% in treated group whereas in placebo group the infected patients decreased from 31.25% to 20.00%. The viral reduction in treated group indicated the benefit of the product. Insignificant result might arise from a low number of participated patients and insufficient concentration of plant extract in herbal product.