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Intracellular Regulation of Cross-Presentation during Dendritic Cell Maturation
We have investigated the effect of different maturation stimuli on the ability of mature dendritic cells (DCs) to cross-present newly acquired particulate antigens. Cross-presentation was impaired in DCs matured by treatment with TNF-α, CpG and LPS, but was less affected upon CD40L-induced maturatio...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3789698/ https://www.ncbi.nlm.nih.gov/pubmed/24098562 http://dx.doi.org/10.1371/journal.pone.0076801 |
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author | Wagner, Claudia S. Grotzke, Jeff Cresswell, Peter |
author_facet | Wagner, Claudia S. Grotzke, Jeff Cresswell, Peter |
author_sort | Wagner, Claudia S. |
collection | PubMed |
description | We have investigated the effect of different maturation stimuli on the ability of mature dendritic cells (DCs) to cross-present newly acquired particulate antigens. Cross-presentation was impaired in DCs matured by treatment with TNF-α, CpG and LPS, but was less affected upon CD40L-induced maturation. The difference could not be explained by decreased antigen uptake or translocation into the cytosol, but decreased cross-presentation ability did correlate with increased phagosomal/lysosomal acidification. Nevertheless, intra-phagosomal degradation of OVA was not increased in matured samples, suggesting that decreasing phagosomal pH may also regulate cross-presentation by a mechanism other than enhancing degradation. |
format | Online Article Text |
id | pubmed-3789698 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-37896982013-10-04 Intracellular Regulation of Cross-Presentation during Dendritic Cell Maturation Wagner, Claudia S. Grotzke, Jeff Cresswell, Peter PLoS One Research Article We have investigated the effect of different maturation stimuli on the ability of mature dendritic cells (DCs) to cross-present newly acquired particulate antigens. Cross-presentation was impaired in DCs matured by treatment with TNF-α, CpG and LPS, but was less affected upon CD40L-induced maturation. The difference could not be explained by decreased antigen uptake or translocation into the cytosol, but decreased cross-presentation ability did correlate with increased phagosomal/lysosomal acidification. Nevertheless, intra-phagosomal degradation of OVA was not increased in matured samples, suggesting that decreasing phagosomal pH may also regulate cross-presentation by a mechanism other than enhancing degradation. Public Library of Science 2013-10-03 /pmc/articles/PMC3789698/ /pubmed/24098562 http://dx.doi.org/10.1371/journal.pone.0076801 Text en © 2013 Wagner et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Wagner, Claudia S. Grotzke, Jeff Cresswell, Peter Intracellular Regulation of Cross-Presentation during Dendritic Cell Maturation |
title | Intracellular Regulation of Cross-Presentation during Dendritic Cell Maturation |
title_full | Intracellular Regulation of Cross-Presentation during Dendritic Cell Maturation |
title_fullStr | Intracellular Regulation of Cross-Presentation during Dendritic Cell Maturation |
title_full_unstemmed | Intracellular Regulation of Cross-Presentation during Dendritic Cell Maturation |
title_short | Intracellular Regulation of Cross-Presentation during Dendritic Cell Maturation |
title_sort | intracellular regulation of cross-presentation during dendritic cell maturation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3789698/ https://www.ncbi.nlm.nih.gov/pubmed/24098562 http://dx.doi.org/10.1371/journal.pone.0076801 |
work_keys_str_mv | AT wagnerclaudias intracellularregulationofcrosspresentationduringdendriticcellmaturation AT grotzkejeff intracellularregulationofcrosspresentationduringdendriticcellmaturation AT cresswellpeter intracellularregulationofcrosspresentationduringdendriticcellmaturation |