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Oral Community Interactions of Filifactor alocis In Vitro

Filifactor alocis is a gram positive anaerobe that is emerging as an important periodontal pathogen. In the oral cavity F. alocis colonizes polymicrobial biofilm communities; however, little is known regarding the nature of the interactions between F. alocis and other oral biofilm bacteria. Here we...

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Autores principales: Wang, Qian, Wright, Christopher J., Dingming, Huang, Uriarte, Silvia M., Lamont, Richard J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3789735/
https://www.ncbi.nlm.nih.gov/pubmed/24098460
http://dx.doi.org/10.1371/journal.pone.0076271
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author Wang, Qian
Wright, Christopher J.
Dingming, Huang
Uriarte, Silvia M.
Lamont, Richard J.
author_facet Wang, Qian
Wright, Christopher J.
Dingming, Huang
Uriarte, Silvia M.
Lamont, Richard J.
author_sort Wang, Qian
collection PubMed
description Filifactor alocis is a gram positive anaerobe that is emerging as an important periodontal pathogen. In the oral cavity F. alocis colonizes polymicrobial biofilm communities; however, little is known regarding the nature of the interactions between F. alocis and other oral biofilm bacteria. Here we investigate the community interactions of two strains of F. alocis with Streptococcus gordonii, Fusobacterium nucleatum, Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans, organisms with differing pathogenic potential in the oral cavity. In an in vitro community development model, S. gordonii was antagonistic to the accumulation of F. alocis into a dual species community. In contrast, F. nucleatum and the type strain of F. alocis formed a synergistic partnership. Accumulation of a low passage isolate of F. alocis was also enhanced by F. nucleatum. In three species communities of S. gordonii, F. nucleatum and F. alocis, the antagonistic effects of S. gordonii superseded the synergistic effects of F. nucleatum toward F. alocis. The interaction between A. actinomycetemcomitans and F. alocis was strain specific and A. actinomycetemcomitans could either stimulate F. alocis accumulation or have no effect depending on the strain. P. gingivalis and F. alocis formed heterotypic communities with the amount of P. gingivalis greater than in the absence of F. alocis. However, while P. gingivalis benefited from the relationship, levels of F. alocis in the dual species community were lower compared to F. alocis alone. The inhibitory effect of P. gingivalis toward F. alocis was dependent, at least partially, on the presence of the Mfa1 fimbrial subunit. In addition, AI-2 production by P. gingivalis helped maintain levels of F. alocis. Collectively, these results show that the pattern of F. alocis colonization will be dictated by the spatial composition of microbial microenvironments, and that the organism may preferentially accumulate at sites rich in F. nucleatum.
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spelling pubmed-37897352013-10-04 Oral Community Interactions of Filifactor alocis In Vitro Wang, Qian Wright, Christopher J. Dingming, Huang Uriarte, Silvia M. Lamont, Richard J. PLoS One Research Article Filifactor alocis is a gram positive anaerobe that is emerging as an important periodontal pathogen. In the oral cavity F. alocis colonizes polymicrobial biofilm communities; however, little is known regarding the nature of the interactions between F. alocis and other oral biofilm bacteria. Here we investigate the community interactions of two strains of F. alocis with Streptococcus gordonii, Fusobacterium nucleatum, Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans, organisms with differing pathogenic potential in the oral cavity. In an in vitro community development model, S. gordonii was antagonistic to the accumulation of F. alocis into a dual species community. In contrast, F. nucleatum and the type strain of F. alocis formed a synergistic partnership. Accumulation of a low passage isolate of F. alocis was also enhanced by F. nucleatum. In three species communities of S. gordonii, F. nucleatum and F. alocis, the antagonistic effects of S. gordonii superseded the synergistic effects of F. nucleatum toward F. alocis. The interaction between A. actinomycetemcomitans and F. alocis was strain specific and A. actinomycetemcomitans could either stimulate F. alocis accumulation or have no effect depending on the strain. P. gingivalis and F. alocis formed heterotypic communities with the amount of P. gingivalis greater than in the absence of F. alocis. However, while P. gingivalis benefited from the relationship, levels of F. alocis in the dual species community were lower compared to F. alocis alone. The inhibitory effect of P. gingivalis toward F. alocis was dependent, at least partially, on the presence of the Mfa1 fimbrial subunit. In addition, AI-2 production by P. gingivalis helped maintain levels of F. alocis. Collectively, these results show that the pattern of F. alocis colonization will be dictated by the spatial composition of microbial microenvironments, and that the organism may preferentially accumulate at sites rich in F. nucleatum. Public Library of Science 2013-10-03 /pmc/articles/PMC3789735/ /pubmed/24098460 http://dx.doi.org/10.1371/journal.pone.0076271 Text en © 2013 Wang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Wang, Qian
Wright, Christopher J.
Dingming, Huang
Uriarte, Silvia M.
Lamont, Richard J.
Oral Community Interactions of Filifactor alocis In Vitro
title Oral Community Interactions of Filifactor alocis In Vitro
title_full Oral Community Interactions of Filifactor alocis In Vitro
title_fullStr Oral Community Interactions of Filifactor alocis In Vitro
title_full_unstemmed Oral Community Interactions of Filifactor alocis In Vitro
title_short Oral Community Interactions of Filifactor alocis In Vitro
title_sort oral community interactions of filifactor alocis in vitro
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3789735/
https://www.ncbi.nlm.nih.gov/pubmed/24098460
http://dx.doi.org/10.1371/journal.pone.0076271
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