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Expression Plasmids for Use in Candida glabrata
We describe a series of CEN/ARS episomal plasmids containing different Candida glabrata promoters, allowing for a range of constitutive or regulated expression of proteins in C. glabrata. The set of promoters includes three constitutive promoters (EGD2pr, HHT2pr, PDC1pr), two macrophage/phagocytosis...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Genetics Society of America
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3789792/ https://www.ncbi.nlm.nih.gov/pubmed/23934995 http://dx.doi.org/10.1534/g3.113.006908 |
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author | Zordan, Rebecca E. Ren, Yuxia Pan, Shih-Jung Rotondo, Giuseppe Peñas, Alejandro De Las Iluore, Joseph Cormack, Brendan P. |
author_facet | Zordan, Rebecca E. Ren, Yuxia Pan, Shih-Jung Rotondo, Giuseppe Peñas, Alejandro De Las Iluore, Joseph Cormack, Brendan P. |
author_sort | Zordan, Rebecca E. |
collection | PubMed |
description | We describe a series of CEN/ARS episomal plasmids containing different Candida glabrata promoters, allowing for a range of constitutive or regulated expression of proteins in C. glabrata. The set of promoters includes three constitutive promoters (EGD2pr, HHT2pr, PDC1pr), two macrophage/phagocytosis-induced promoters (ACO2pr, LYS21pr), and one nutritionally regulated promoter (MET3pr). Each promoter was cloned into two plasmid backbones that differ in their selectable marker, URA3, or the dominant-selectable NAT1 gene, which confers resistance to the drug nourseothricin. Expression from the 12 resulting plasmids was assessed using GFP as a reporter and flow cytometry or quantitative reverse-transcription polymerase chain reaction to assess expression levels. Together this set of plasmids expands the toolkit of expression vectors available for use with C. glabrata. |
format | Online Article Text |
id | pubmed-3789792 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Genetics Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-37897922013-10-17 Expression Plasmids for Use in Candida glabrata Zordan, Rebecca E. Ren, Yuxia Pan, Shih-Jung Rotondo, Giuseppe Peñas, Alejandro De Las Iluore, Joseph Cormack, Brendan P. G3 (Bethesda) Investigations We describe a series of CEN/ARS episomal plasmids containing different Candida glabrata promoters, allowing for a range of constitutive or regulated expression of proteins in C. glabrata. The set of promoters includes three constitutive promoters (EGD2pr, HHT2pr, PDC1pr), two macrophage/phagocytosis-induced promoters (ACO2pr, LYS21pr), and one nutritionally regulated promoter (MET3pr). Each promoter was cloned into two plasmid backbones that differ in their selectable marker, URA3, or the dominant-selectable NAT1 gene, which confers resistance to the drug nourseothricin. Expression from the 12 resulting plasmids was assessed using GFP as a reporter and flow cytometry or quantitative reverse-transcription polymerase chain reaction to assess expression levels. Together this set of plasmids expands the toolkit of expression vectors available for use with C. glabrata. Genetics Society of America 2013-10-01 /pmc/articles/PMC3789792/ /pubmed/23934995 http://dx.doi.org/10.1534/g3.113.006908 Text en Copyright © 2013 Zordan et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Unported License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Investigations Zordan, Rebecca E. Ren, Yuxia Pan, Shih-Jung Rotondo, Giuseppe Peñas, Alejandro De Las Iluore, Joseph Cormack, Brendan P. Expression Plasmids for Use in Candida glabrata |
title | Expression Plasmids for Use in Candida glabrata |
title_full | Expression Plasmids for Use in Candida glabrata |
title_fullStr | Expression Plasmids for Use in Candida glabrata |
title_full_unstemmed | Expression Plasmids for Use in Candida glabrata |
title_short | Expression Plasmids for Use in Candida glabrata |
title_sort | expression plasmids for use in candida glabrata |
topic | Investigations |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3789792/ https://www.ncbi.nlm.nih.gov/pubmed/23934995 http://dx.doi.org/10.1534/g3.113.006908 |
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