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Disruption of the Rice Plastid Ribosomal Protein S20 Leads to Chloroplast Developmental Defects and Seedling Lethality

Plastid ribosomal proteins (PRPs) are essential for ribosome biogenesis, plastid protein biosynthesis, chloroplast differentiation, and early chloroplast development. This study identifies the first rice PRP mutant, asl1 (albino seedling lethality1), which exhibits an albino lethal phenotype at the...

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Autores principales: Gong, Xiaodi, Jiang, Quan, Xu, Jianlong, Zhang, Jianhui, Teng, Sheng, Lin, Dongzhi, Dong, Yanjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3789801/
https://www.ncbi.nlm.nih.gov/pubmed/23979931
http://dx.doi.org/10.1534/g3.113.007856
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author Gong, Xiaodi
Jiang, Quan
Xu, Jianlong
Zhang, Jianhui
Teng, Sheng
Lin, Dongzhi
Dong, Yanjun
author_facet Gong, Xiaodi
Jiang, Quan
Xu, Jianlong
Zhang, Jianhui
Teng, Sheng
Lin, Dongzhi
Dong, Yanjun
author_sort Gong, Xiaodi
collection PubMed
description Plastid ribosomal proteins (PRPs) are essential for ribosome biogenesis, plastid protein biosynthesis, chloroplast differentiation, and early chloroplast development. This study identifies the first rice PRP mutant, asl1 (albino seedling lethality1), which exhibits an albino lethal phenotype at the seedling stage. This albino phenotype was associated with altered chlorophyll (Chl) content and chloroplast development. Map-based cloning revealed that ASL1 encodes PRP S20 (PRPS20), which localizes to the chloroplast. ASL1 showed tissue-specific expression, as it was highly expressed in plumule and young seedlings but expressed at much lower levels in other tissues. In addition, ASL1 expression was regulated by light. The transcript levels of nuclear genes for Chl biosynthesis and chloroplast development were strongly affected in asl1 mutants; transcripts of some plastid genes for photosynthesis were undetectable. Our findings indicate that nuclear-encoded PRPS20 plays an important role in chloroplast development in rice.
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spelling pubmed-37898012013-10-17 Disruption of the Rice Plastid Ribosomal Protein S20 Leads to Chloroplast Developmental Defects and Seedling Lethality Gong, Xiaodi Jiang, Quan Xu, Jianlong Zhang, Jianhui Teng, Sheng Lin, Dongzhi Dong, Yanjun G3 (Bethesda) Investigations Plastid ribosomal proteins (PRPs) are essential for ribosome biogenesis, plastid protein biosynthesis, chloroplast differentiation, and early chloroplast development. This study identifies the first rice PRP mutant, asl1 (albino seedling lethality1), which exhibits an albino lethal phenotype at the seedling stage. This albino phenotype was associated with altered chlorophyll (Chl) content and chloroplast development. Map-based cloning revealed that ASL1 encodes PRP S20 (PRPS20), which localizes to the chloroplast. ASL1 showed tissue-specific expression, as it was highly expressed in plumule and young seedlings but expressed at much lower levels in other tissues. In addition, ASL1 expression was regulated by light. The transcript levels of nuclear genes for Chl biosynthesis and chloroplast development were strongly affected in asl1 mutants; transcripts of some plastid genes for photosynthesis were undetectable. Our findings indicate that nuclear-encoded PRPS20 plays an important role in chloroplast development in rice. Genetics Society of America 2013-10-01 /pmc/articles/PMC3789801/ /pubmed/23979931 http://dx.doi.org/10.1534/g3.113.007856 Text en Copyright © 2013 Gong et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Unported License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Investigations
Gong, Xiaodi
Jiang, Quan
Xu, Jianlong
Zhang, Jianhui
Teng, Sheng
Lin, Dongzhi
Dong, Yanjun
Disruption of the Rice Plastid Ribosomal Protein S20 Leads to Chloroplast Developmental Defects and Seedling Lethality
title Disruption of the Rice Plastid Ribosomal Protein S20 Leads to Chloroplast Developmental Defects and Seedling Lethality
title_full Disruption of the Rice Plastid Ribosomal Protein S20 Leads to Chloroplast Developmental Defects and Seedling Lethality
title_fullStr Disruption of the Rice Plastid Ribosomal Protein S20 Leads to Chloroplast Developmental Defects and Seedling Lethality
title_full_unstemmed Disruption of the Rice Plastid Ribosomal Protein S20 Leads to Chloroplast Developmental Defects and Seedling Lethality
title_short Disruption of the Rice Plastid Ribosomal Protein S20 Leads to Chloroplast Developmental Defects and Seedling Lethality
title_sort disruption of the rice plastid ribosomal protein s20 leads to chloroplast developmental defects and seedling lethality
topic Investigations
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3789801/
https://www.ncbi.nlm.nih.gov/pubmed/23979931
http://dx.doi.org/10.1534/g3.113.007856
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