Cargando…

Chimeric Protein Complexes in Hybrid Species Generate Novel Phenotypes

Hybridization between species is an important mechanism for the origin of novel lineages and adaptation to new environments. Increased allelic variation and modification of the transcriptional network are the two recognized forces currently deemed to be responsible for the phenotypic properties seen...

Descripción completa

Detalles Bibliográficos
Autores principales: Piatkowska, Elzbieta M., Naseeb, Samina, Knight, David, Delneri, Daniela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3789821/
https://www.ncbi.nlm.nih.gov/pubmed/24137105
http://dx.doi.org/10.1371/journal.pgen.1003836
_version_ 1782286507547033600
author Piatkowska, Elzbieta M.
Naseeb, Samina
Knight, David
Delneri, Daniela
author_facet Piatkowska, Elzbieta M.
Naseeb, Samina
Knight, David
Delneri, Daniela
author_sort Piatkowska, Elzbieta M.
collection PubMed
description Hybridization between species is an important mechanism for the origin of novel lineages and adaptation to new environments. Increased allelic variation and modification of the transcriptional network are the two recognized forces currently deemed to be responsible for the phenotypic properties seen in hybrids. However, since the majority of the biological functions in a cell are carried out by protein complexes, inter-specific protein assemblies therefore represent another important source of natural variation upon which evolutionary forces can act. Here we studied the composition of six protein complexes in two different Saccharomyces “sensu stricto” hybrids, to understand whether chimeric interactions can be freely formed in the cell in spite of species-specific co-evolutionary forces, and whether the different types of complexes cause a change in hybrid fitness. The protein assemblies were isolated from the hybrids via affinity chromatography and identified via mass spectrometry. We found evidence of spontaneous chimericity for four of the six protein assemblies tested and we showed that different types of complexes can cause a variety of phenotypes in selected environments. In the case of TRP2/TRP3 complex, the effect of such chimeric formation resulted in the fitness advantage of the hybrid in an environment lacking tryptophan, while only one type of parental combination of the MBF complex allowed the hybrid to grow under respiratory conditions. These phenotypes were dependent on both genetic and environmental backgrounds. This study provides empirical evidence that chimeric protein complexes can freely assemble in cells and reveals a new mechanism to generate phenotypic novelty and plasticity in hybrids to complement the genomic innovation resulting from gene duplication. The ability to exchange orthologous members has also important implications for the adaptation and subsequent genome evolution of the hybrids in terms of pattern of gene loss.
format Online
Article
Text
id pubmed-3789821
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-37898212013-10-17 Chimeric Protein Complexes in Hybrid Species Generate Novel Phenotypes Piatkowska, Elzbieta M. Naseeb, Samina Knight, David Delneri, Daniela PLoS Genet Research Article Hybridization between species is an important mechanism for the origin of novel lineages and adaptation to new environments. Increased allelic variation and modification of the transcriptional network are the two recognized forces currently deemed to be responsible for the phenotypic properties seen in hybrids. However, since the majority of the biological functions in a cell are carried out by protein complexes, inter-specific protein assemblies therefore represent another important source of natural variation upon which evolutionary forces can act. Here we studied the composition of six protein complexes in two different Saccharomyces “sensu stricto” hybrids, to understand whether chimeric interactions can be freely formed in the cell in spite of species-specific co-evolutionary forces, and whether the different types of complexes cause a change in hybrid fitness. The protein assemblies were isolated from the hybrids via affinity chromatography and identified via mass spectrometry. We found evidence of spontaneous chimericity for four of the six protein assemblies tested and we showed that different types of complexes can cause a variety of phenotypes in selected environments. In the case of TRP2/TRP3 complex, the effect of such chimeric formation resulted in the fitness advantage of the hybrid in an environment lacking tryptophan, while only one type of parental combination of the MBF complex allowed the hybrid to grow under respiratory conditions. These phenotypes were dependent on both genetic and environmental backgrounds. This study provides empirical evidence that chimeric protein complexes can freely assemble in cells and reveals a new mechanism to generate phenotypic novelty and plasticity in hybrids to complement the genomic innovation resulting from gene duplication. The ability to exchange orthologous members has also important implications for the adaptation and subsequent genome evolution of the hybrids in terms of pattern of gene loss. Public Library of Science 2013-10-03 /pmc/articles/PMC3789821/ /pubmed/24137105 http://dx.doi.org/10.1371/journal.pgen.1003836 Text en © 2013 Piatkowska et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Piatkowska, Elzbieta M.
Naseeb, Samina
Knight, David
Delneri, Daniela
Chimeric Protein Complexes in Hybrid Species Generate Novel Phenotypes
title Chimeric Protein Complexes in Hybrid Species Generate Novel Phenotypes
title_full Chimeric Protein Complexes in Hybrid Species Generate Novel Phenotypes
title_fullStr Chimeric Protein Complexes in Hybrid Species Generate Novel Phenotypes
title_full_unstemmed Chimeric Protein Complexes in Hybrid Species Generate Novel Phenotypes
title_short Chimeric Protein Complexes in Hybrid Species Generate Novel Phenotypes
title_sort chimeric protein complexes in hybrid species generate novel phenotypes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3789821/
https://www.ncbi.nlm.nih.gov/pubmed/24137105
http://dx.doi.org/10.1371/journal.pgen.1003836
work_keys_str_mv AT piatkowskaelzbietam chimericproteincomplexesinhybridspeciesgeneratenovelphenotypes
AT naseebsamina chimericproteincomplexesinhybridspeciesgeneratenovelphenotypes
AT knightdavid chimericproteincomplexesinhybridspeciesgeneratenovelphenotypes
AT delneridaniela chimericproteincomplexesinhybridspeciesgeneratenovelphenotypes