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Microbead-based biomimetic synthetic neighbors enhance survival and function of rat pancreatic β-cells
Diabetes is caused by the loss or dysfunction of insulin-secreting β-cells in the pancreas. β-cells reduce their mass and lose insulin-producing ability in vitro, likely due to insufficient cell-cell and cell-extracellular matrix (ECM) interactions as β-cells lose their native microenvironment. Here...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3790197/ https://www.ncbi.nlm.nih.gov/pubmed/24091640 http://dx.doi.org/10.1038/srep02863 |
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author | Li, Wei Lee, Samuel Ma, Minglin Kim, Soo Min Guye, Patrick Pancoast, James R. Anderson, Daniel G. Weiss, Ron Lee, Richard T. Hammond, Paula T. |
author_facet | Li, Wei Lee, Samuel Ma, Minglin Kim, Soo Min Guye, Patrick Pancoast, James R. Anderson, Daniel G. Weiss, Ron Lee, Richard T. Hammond, Paula T. |
author_sort | Li, Wei |
collection | PubMed |
description | Diabetes is caused by the loss or dysfunction of insulin-secreting β-cells in the pancreas. β-cells reduce their mass and lose insulin-producing ability in vitro, likely due to insufficient cell-cell and cell-extracellular matrix (ECM) interactions as β-cells lose their native microenvironment. Herein, we built an ex-vivo cell microenvironment by culturing primary β-cells in direct contact with ‘synthetic neighbors', cell-sized soft polymer microbeads that were modified with cell-cell signaling factors as well as components from pancreatic-tissue-specific ECMs. This biomimetic 3D microenvironment was able to promote native cell-cell and cell-ECM interactions. We obtained sustained maintenance of β-cell function in vitro enhanced cell viability from the few days usually observed in 2D culture to periods exceeding three weeks, with enhanced β-cell stability and insulin production. Our approach can be extended to create a general 3D culture platform for other cell types. |
format | Online Article Text |
id | pubmed-3790197 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-37901972013-10-18 Microbead-based biomimetic synthetic neighbors enhance survival and function of rat pancreatic β-cells Li, Wei Lee, Samuel Ma, Minglin Kim, Soo Min Guye, Patrick Pancoast, James R. Anderson, Daniel G. Weiss, Ron Lee, Richard T. Hammond, Paula T. Sci Rep Article Diabetes is caused by the loss or dysfunction of insulin-secreting β-cells in the pancreas. β-cells reduce their mass and lose insulin-producing ability in vitro, likely due to insufficient cell-cell and cell-extracellular matrix (ECM) interactions as β-cells lose their native microenvironment. Herein, we built an ex-vivo cell microenvironment by culturing primary β-cells in direct contact with ‘synthetic neighbors', cell-sized soft polymer microbeads that were modified with cell-cell signaling factors as well as components from pancreatic-tissue-specific ECMs. This biomimetic 3D microenvironment was able to promote native cell-cell and cell-ECM interactions. We obtained sustained maintenance of β-cell function in vitro enhanced cell viability from the few days usually observed in 2D culture to periods exceeding three weeks, with enhanced β-cell stability and insulin production. Our approach can be extended to create a general 3D culture platform for other cell types. Nature Publishing Group 2013-10-04 /pmc/articles/PMC3790197/ /pubmed/24091640 http://dx.doi.org/10.1038/srep02863 Text en Copyright © 2013, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/ |
spellingShingle | Article Li, Wei Lee, Samuel Ma, Minglin Kim, Soo Min Guye, Patrick Pancoast, James R. Anderson, Daniel G. Weiss, Ron Lee, Richard T. Hammond, Paula T. Microbead-based biomimetic synthetic neighbors enhance survival and function of rat pancreatic β-cells |
title | Microbead-based biomimetic synthetic neighbors enhance survival and function of rat pancreatic β-cells |
title_full | Microbead-based biomimetic synthetic neighbors enhance survival and function of rat pancreatic β-cells |
title_fullStr | Microbead-based biomimetic synthetic neighbors enhance survival and function of rat pancreatic β-cells |
title_full_unstemmed | Microbead-based biomimetic synthetic neighbors enhance survival and function of rat pancreatic β-cells |
title_short | Microbead-based biomimetic synthetic neighbors enhance survival and function of rat pancreatic β-cells |
title_sort | microbead-based biomimetic synthetic neighbors enhance survival and function of rat pancreatic β-cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3790197/ https://www.ncbi.nlm.nih.gov/pubmed/24091640 http://dx.doi.org/10.1038/srep02863 |
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