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Expression, Purification and Low-Resolution Structure of Human Vitamin C Transporter SVCT1 (SLC23A1)
Expression and purification of human membrane proteins for structural studies represent a great challenge. This is because micro- to milligram amounts of pure isolated protein are required. To this aim, we successfully expressed the human vitamin C transporter-1 (hSVCT1; SLC23A1) in Xenopus laevis o...
Autores principales: | Boggavarapu, Rajendra, Jeckelmann, Jean-Marc, Harder, Daniel, Schneider, Philipp, Ucurum, Zöhre, Hediger, Matthias, Fotiadis, Dimitrios |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3790709/ https://www.ncbi.nlm.nih.gov/pubmed/24124560 http://dx.doi.org/10.1371/journal.pone.0076427 |
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