Ewing Sarcoma Cells Secrete EWS/Fli-1 Fusion mRNA via Microvesicles
Tumours defined as Ewing sarcoma (ES) constitute a group of highly malignant neoplasms that most often affect children and young adults in the first 2 decades of life. The EWS/Fli-1 fusion gene, a product of the translocation t(11;22) (q24; 12), is detected in 95% of ES patients. Recently, it was va...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3790721/ https://www.ncbi.nlm.nih.gov/pubmed/24124617 http://dx.doi.org/10.1371/journal.pone.0077416 |
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author | Tsugita, Masanori Yamada, Nami Noguchi, Shunsuke Yamada, Kazunari Moritake, Hiroshi Shimizu, Katsuji Akao, Yukihiro Ohno, Takatoshi |
author_facet | Tsugita, Masanori Yamada, Nami Noguchi, Shunsuke Yamada, Kazunari Moritake, Hiroshi Shimizu, Katsuji Akao, Yukihiro Ohno, Takatoshi |
author_sort | Tsugita, Masanori |
collection | PubMed |
description | Tumours defined as Ewing sarcoma (ES) constitute a group of highly malignant neoplasms that most often affect children and young adults in the first 2 decades of life. The EWS/Fli-1 fusion gene, a product of the translocation t(11;22) (q24; 12), is detected in 95% of ES patients. Recently, it was validated that cells emit a heterogeneous mixture of vesicular, organelle-like structures (microvesicles, MVs) into their surroundings including blood and body fluids, and that these MVs contain a selected set of tumor-related proteins and high levels of mRNAs and miRNAs. In this present study, we detected the Ewing sarcoma-specific EWS/Fli-1 mRNA in MVs from the culture medium of ES cell lines carrying t(11;22) (q24; 12). Also, we detected this fusion gene in approximately 40% of the blood samples from mice inoculated with xenografts of TC135 or A673 cells. These findings indicate the EWS/Fli-1 mRNA in MVs might be a new non-invasive diagnostic marker for specific cases of Ewing sarcoma. |
format | Online Article Text |
id | pubmed-3790721 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-37907212013-10-11 Ewing Sarcoma Cells Secrete EWS/Fli-1 Fusion mRNA via Microvesicles Tsugita, Masanori Yamada, Nami Noguchi, Shunsuke Yamada, Kazunari Moritake, Hiroshi Shimizu, Katsuji Akao, Yukihiro Ohno, Takatoshi PLoS One Research Article Tumours defined as Ewing sarcoma (ES) constitute a group of highly malignant neoplasms that most often affect children and young adults in the first 2 decades of life. The EWS/Fli-1 fusion gene, a product of the translocation t(11;22) (q24; 12), is detected in 95% of ES patients. Recently, it was validated that cells emit a heterogeneous mixture of vesicular, organelle-like structures (microvesicles, MVs) into their surroundings including blood and body fluids, and that these MVs contain a selected set of tumor-related proteins and high levels of mRNAs and miRNAs. In this present study, we detected the Ewing sarcoma-specific EWS/Fli-1 mRNA in MVs from the culture medium of ES cell lines carrying t(11;22) (q24; 12). Also, we detected this fusion gene in approximately 40% of the blood samples from mice inoculated with xenografts of TC135 or A673 cells. These findings indicate the EWS/Fli-1 mRNA in MVs might be a new non-invasive diagnostic marker for specific cases of Ewing sarcoma. Public Library of Science 2013-10-04 /pmc/articles/PMC3790721/ /pubmed/24124617 http://dx.doi.org/10.1371/journal.pone.0077416 Text en © 2013 Tsugita et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Tsugita, Masanori Yamada, Nami Noguchi, Shunsuke Yamada, Kazunari Moritake, Hiroshi Shimizu, Katsuji Akao, Yukihiro Ohno, Takatoshi Ewing Sarcoma Cells Secrete EWS/Fli-1 Fusion mRNA via Microvesicles |
title | Ewing Sarcoma Cells Secrete EWS/Fli-1 Fusion mRNA via Microvesicles |
title_full | Ewing Sarcoma Cells Secrete EWS/Fli-1 Fusion mRNA via Microvesicles |
title_fullStr | Ewing Sarcoma Cells Secrete EWS/Fli-1 Fusion mRNA via Microvesicles |
title_full_unstemmed | Ewing Sarcoma Cells Secrete EWS/Fli-1 Fusion mRNA via Microvesicles |
title_short | Ewing Sarcoma Cells Secrete EWS/Fli-1 Fusion mRNA via Microvesicles |
title_sort | ewing sarcoma cells secrete ews/fli-1 fusion mrna via microvesicles |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3790721/ https://www.ncbi.nlm.nih.gov/pubmed/24124617 http://dx.doi.org/10.1371/journal.pone.0077416 |
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