Ewing Sarcoma Cells Secrete EWS/Fli-1 Fusion mRNA via Microvesicles

Tumours defined as Ewing sarcoma (ES) constitute a group of highly malignant neoplasms that most often affect children and young adults in the first 2 decades of life. The EWS/Fli-1 fusion gene, a product of the translocation t(11;22) (q24; 12), is detected in 95% of ES patients. Recently, it was va...

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Autores principales: Tsugita, Masanori, Yamada, Nami, Noguchi, Shunsuke, Yamada, Kazunari, Moritake, Hiroshi, Shimizu, Katsuji, Akao, Yukihiro, Ohno, Takatoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3790721/
https://www.ncbi.nlm.nih.gov/pubmed/24124617
http://dx.doi.org/10.1371/journal.pone.0077416
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author Tsugita, Masanori
Yamada, Nami
Noguchi, Shunsuke
Yamada, Kazunari
Moritake, Hiroshi
Shimizu, Katsuji
Akao, Yukihiro
Ohno, Takatoshi
author_facet Tsugita, Masanori
Yamada, Nami
Noguchi, Shunsuke
Yamada, Kazunari
Moritake, Hiroshi
Shimizu, Katsuji
Akao, Yukihiro
Ohno, Takatoshi
author_sort Tsugita, Masanori
collection PubMed
description Tumours defined as Ewing sarcoma (ES) constitute a group of highly malignant neoplasms that most often affect children and young adults in the first 2 decades of life. The EWS/Fli-1 fusion gene, a product of the translocation t(11;22) (q24; 12), is detected in 95% of ES patients. Recently, it was validated that cells emit a heterogeneous mixture of vesicular, organelle-like structures (microvesicles, MVs) into their surroundings including blood and body fluids, and that these MVs contain a selected set of tumor-related proteins and high levels of mRNAs and miRNAs. In this present study, we detected the Ewing sarcoma-specific EWS/Fli-1 mRNA in MVs from the culture medium of ES cell lines carrying t(11;22) (q24; 12). Also, we detected this fusion gene in approximately 40% of the blood samples from mice inoculated with xenografts of TC135 or A673 cells. These findings indicate the EWS/Fli-1 mRNA in MVs might be a new non-invasive diagnostic marker for specific cases of Ewing sarcoma.
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spelling pubmed-37907212013-10-11 Ewing Sarcoma Cells Secrete EWS/Fli-1 Fusion mRNA via Microvesicles Tsugita, Masanori Yamada, Nami Noguchi, Shunsuke Yamada, Kazunari Moritake, Hiroshi Shimizu, Katsuji Akao, Yukihiro Ohno, Takatoshi PLoS One Research Article Tumours defined as Ewing sarcoma (ES) constitute a group of highly malignant neoplasms that most often affect children and young adults in the first 2 decades of life. The EWS/Fli-1 fusion gene, a product of the translocation t(11;22) (q24; 12), is detected in 95% of ES patients. Recently, it was validated that cells emit a heterogeneous mixture of vesicular, organelle-like structures (microvesicles, MVs) into their surroundings including blood and body fluids, and that these MVs contain a selected set of tumor-related proteins and high levels of mRNAs and miRNAs. In this present study, we detected the Ewing sarcoma-specific EWS/Fli-1 mRNA in MVs from the culture medium of ES cell lines carrying t(11;22) (q24; 12). Also, we detected this fusion gene in approximately 40% of the blood samples from mice inoculated with xenografts of TC135 or A673 cells. These findings indicate the EWS/Fli-1 mRNA in MVs might be a new non-invasive diagnostic marker for specific cases of Ewing sarcoma. Public Library of Science 2013-10-04 /pmc/articles/PMC3790721/ /pubmed/24124617 http://dx.doi.org/10.1371/journal.pone.0077416 Text en © 2013 Tsugita et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Tsugita, Masanori
Yamada, Nami
Noguchi, Shunsuke
Yamada, Kazunari
Moritake, Hiroshi
Shimizu, Katsuji
Akao, Yukihiro
Ohno, Takatoshi
Ewing Sarcoma Cells Secrete EWS/Fli-1 Fusion mRNA via Microvesicles
title Ewing Sarcoma Cells Secrete EWS/Fli-1 Fusion mRNA via Microvesicles
title_full Ewing Sarcoma Cells Secrete EWS/Fli-1 Fusion mRNA via Microvesicles
title_fullStr Ewing Sarcoma Cells Secrete EWS/Fli-1 Fusion mRNA via Microvesicles
title_full_unstemmed Ewing Sarcoma Cells Secrete EWS/Fli-1 Fusion mRNA via Microvesicles
title_short Ewing Sarcoma Cells Secrete EWS/Fli-1 Fusion mRNA via Microvesicles
title_sort ewing sarcoma cells secrete ews/fli-1 fusion mrna via microvesicles
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3790721/
https://www.ncbi.nlm.nih.gov/pubmed/24124617
http://dx.doi.org/10.1371/journal.pone.0077416
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