A “Clickable” MTX Reagent as a Practical Tool for Profiling Small-Molecule–Intracellular Target Interactions via MASPIT

We present a scalable synthesis of a versatile MTX reagent with an azide ligation handle that allows rapid γ-selective conjugation to yield MTX fusion compounds (MFCs) appropriate for MASPIT, a three-hybrid system that enables the identification of mammalian cytosolic proteins that interact with a s...

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Detalles Bibliográficos
Autores principales: Risseeuw, Martijn D P, De Clercq, Dries J H, Lievens, Sam, Hillaert, Ulrik, Sinnaeve, Davy, Van den Broeck, Freya, Martins, José C, Tavernier, Jan, Van Calenbergh, Serge
Formato: Online Artículo Texto
Lenguaje:English
Publicado: WILEY-VCH Verlag 2013
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Full Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3790973/
https://www.ncbi.nlm.nih.gov/pubmed/23341183
http://dx.doi.org/10.1002/cmdc.201200493

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