Sensitive Aflatoxin B1 Determination Using a Magnetic Particles-Based Enzyme-Linked Immunosorbent Assay

A magnetic particle-based enzyme-liked immunosorbent assay (mp-ELISA) has been developed as new an alternative immunoassay for Aflatoxin B1 determination. The method is based on conventional competitive ELISA whereby the anti-Aflatoxin B1 antibody is immobilized on the magnetic particles' surfa...

Descripción completa

Detalles Bibliográficos
Autores principales: Tudorache, Madalina, Bala, Camelia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2008
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3790977/
https://www.ncbi.nlm.nih.gov/pubmed/27873946
http://dx.doi.org/10.3390/s8127571
_version_ 1782286680022056960
author Tudorache, Madalina
Bala, Camelia
author_facet Tudorache, Madalina
Bala, Camelia
author_sort Tudorache, Madalina
collection PubMed
description A magnetic particle-based enzyme-liked immunosorbent assay (mp-ELISA) has been developed as new an alternative immunoassay for Aflatoxin B1 determination. The method is based on conventional competitive ELISA whereby the anti-Aflatoxin B1 antibody is immobilized on the magnetic particles' surface. The influence of the antibody type as well as antibody immobilization on the magnetic beads surface was investigated in detail. Also, optimum values for the general parameters of the method (e.g. tracer concentration, type of antibody, and incubation time) were established. Finally, a sensitive immunoassay method (mp-ELISA) was performed for Aflatoxin B1 determination at ppt level (LOD = 1 ppt Aflatoxin B1).
format Online
Article
Text
id pubmed-3790977
institution National Center for Biotechnology Information
language English
publishDate 2008
publisher Molecular Diversity Preservation International (MDPI)
record_format MEDLINE/PubMed
spelling pubmed-37909772013-10-18 Sensitive Aflatoxin B1 Determination Using a Magnetic Particles-Based Enzyme-Linked Immunosorbent Assay Tudorache, Madalina Bala, Camelia Sensors (Basel) Article A magnetic particle-based enzyme-liked immunosorbent assay (mp-ELISA) has been developed as new an alternative immunoassay for Aflatoxin B1 determination. The method is based on conventional competitive ELISA whereby the anti-Aflatoxin B1 antibody is immobilized on the magnetic particles' surface. The influence of the antibody type as well as antibody immobilization on the magnetic beads surface was investigated in detail. Also, optimum values for the general parameters of the method (e.g. tracer concentration, type of antibody, and incubation time) were established. Finally, a sensitive immunoassay method (mp-ELISA) was performed for Aflatoxin B1 determination at ppt level (LOD = 1 ppt Aflatoxin B1). Molecular Diversity Preservation International (MDPI) 2008-11-26 /pmc/articles/PMC3790977/ /pubmed/27873946 http://dx.doi.org/10.3390/s8127571 Text en © 2008 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the CreativeCommons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Tudorache, Madalina
Bala, Camelia
Sensitive Aflatoxin B1 Determination Using a Magnetic Particles-Based Enzyme-Linked Immunosorbent Assay
title Sensitive Aflatoxin B1 Determination Using a Magnetic Particles-Based Enzyme-Linked Immunosorbent Assay
title_full Sensitive Aflatoxin B1 Determination Using a Magnetic Particles-Based Enzyme-Linked Immunosorbent Assay
title_fullStr Sensitive Aflatoxin B1 Determination Using a Magnetic Particles-Based Enzyme-Linked Immunosorbent Assay
title_full_unstemmed Sensitive Aflatoxin B1 Determination Using a Magnetic Particles-Based Enzyme-Linked Immunosorbent Assay
title_short Sensitive Aflatoxin B1 Determination Using a Magnetic Particles-Based Enzyme-Linked Immunosorbent Assay
title_sort sensitive aflatoxin b1 determination using a magnetic particles-based enzyme-linked immunosorbent assay
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3790977/
https://www.ncbi.nlm.nih.gov/pubmed/27873946
http://dx.doi.org/10.3390/s8127571
work_keys_str_mv AT tudorachemadalina sensitiveaflatoxinb1determinationusingamagneticparticlesbasedenzymelinkedimmunosorbentassay
AT balacamelia sensitiveaflatoxinb1determinationusingamagneticparticlesbasedenzymelinkedimmunosorbentassay

Ejemplares similares