Vesicles for Signal Amplification in a Biosensor for the Detection of Low Antigen Concentrations

The sensitivity of biosensors is often not sufficient to detect diagnostically relevant biomarker concentrations. In this paper we have utilized a Quartz Crystal Microbalance with Dissipation monitoring (QCM-D) to detect dissipative losses induced by the attachment of intact vesicles. We modified a...

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Autores principales: Grieshaber, Dorothee, de Lange, Victoria, Hirt, Thomas, Lu, Zhihua, Vörös, Janos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2008
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3790996/
https://www.ncbi.nlm.nih.gov/pubmed/27873965
http://dx.doi.org/10.3390/s8127894
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author Grieshaber, Dorothee
de Lange, Victoria
Hirt, Thomas
Lu, Zhihua
Vörös, Janos
author_facet Grieshaber, Dorothee
de Lange, Victoria
Hirt, Thomas
Lu, Zhihua
Vörös, Janos
author_sort Grieshaber, Dorothee
collection PubMed
description The sensitivity of biosensors is often not sufficient to detect diagnostically relevant biomarker concentrations. In this paper we have utilized a Quartz Crystal Microbalance with Dissipation monitoring (QCM-D) to detect dissipative losses induced by the attachment of intact vesicles. We modified a sandwich assay by coupling the secondary antibodies to vesi-cles. This resulted in an increase of detection sensitivity, achieving a diagnostically relevant detection limit of 5 ng/ml or 30 pM antigens. In addition, we could combine the individual assay steps to decrease the total time to result in about 30 minutes.
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spelling pubmed-37909962013-10-18 Vesicles for Signal Amplification in a Biosensor for the Detection of Low Antigen Concentrations Grieshaber, Dorothee de Lange, Victoria Hirt, Thomas Lu, Zhihua Vörös, Janos Sensors (Basel) Article The sensitivity of biosensors is often not sufficient to detect diagnostically relevant biomarker concentrations. In this paper we have utilized a Quartz Crystal Microbalance with Dissipation monitoring (QCM-D) to detect dissipative losses induced by the attachment of intact vesicles. We modified a sandwich assay by coupling the secondary antibodies to vesi-cles. This resulted in an increase of detection sensitivity, achieving a diagnostically relevant detection limit of 5 ng/ml or 30 pM antigens. In addition, we could combine the individual assay steps to decrease the total time to result in about 30 minutes. Molecular Diversity Preservation International (MDPI) 2008-12-05 /pmc/articles/PMC3790996/ /pubmed/27873965 http://dx.doi.org/10.3390/s8127894 Text en © 2008 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative CommonsAttribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Grieshaber, Dorothee
de Lange, Victoria
Hirt, Thomas
Lu, Zhihua
Vörös, Janos
Vesicles for Signal Amplification in a Biosensor for the Detection of Low Antigen Concentrations
title Vesicles for Signal Amplification in a Biosensor for the Detection of Low Antigen Concentrations
title_full Vesicles for Signal Amplification in a Biosensor for the Detection of Low Antigen Concentrations
title_fullStr Vesicles for Signal Amplification in a Biosensor for the Detection of Low Antigen Concentrations
title_full_unstemmed Vesicles for Signal Amplification in a Biosensor for the Detection of Low Antigen Concentrations
title_short Vesicles for Signal Amplification in a Biosensor for the Detection of Low Antigen Concentrations
title_sort vesicles for signal amplification in a biosensor for the detection of low antigen concentrations
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3790996/
https://www.ncbi.nlm.nih.gov/pubmed/27873965
http://dx.doi.org/10.3390/s8127894
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