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Organic Anion Transporting Polypeptide 5A1 (OATP5A1) in Small Cell Lung Cancer (SCLC) Cells: Possible Involvement in Chemoresistance to Satraplatin

BACKGROUND: The role of organic anion transporting polypeptide 5A1 (OATP5A1) a member of a family of drug transporters that mediate cellular uptake of drugs has not been characterized so far. METHODS: Gene expression levels of OATP5A1 in small cell lung cancer (SCLC) cell lines were determined by re...

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Autores principales: Olszewski-Hamilton, U., Svoboda, M., Thalhammer, T., Buxhofer-Ausch, V., Geissler, K., Hamilton, G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Libertas Academica 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3791916/
https://www.ncbi.nlm.nih.gov/pubmed/24179389
http://dx.doi.org/10.4137/BIC.S7151
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author Olszewski-Hamilton, U.
Svoboda, M.
Thalhammer, T.
Buxhofer-Ausch, V.
Geissler, K.
Hamilton, G.
author_facet Olszewski-Hamilton, U.
Svoboda, M.
Thalhammer, T.
Buxhofer-Ausch, V.
Geissler, K.
Hamilton, G.
author_sort Olszewski-Hamilton, U.
collection PubMed
description BACKGROUND: The role of organic anion transporting polypeptide 5A1 (OATP5A1) a member of a family of drug transporters that mediate cellular uptake of drugs has not been characterized so far. METHODS: Gene expression levels of OATP5A1 in small cell lung cancer (SCLC) cell lines were determined by real-time qPCR and chemosensitivity of HEK-293-SLCO5A1-transfected cells to satraplatin in MTT assays. RESULTS: Significant expression of this transporter was found at the mRNA level, primarily in drug-resistant SCLC cells, and SLCO5A1-transfected HEK-293 cells showed higher resistance to satraplatin. OATP5A1 is found preferentially in cytoplasmic membranes of tumor cells, including SCLC. CONCLUSIONS: OATP5A1 seems to effect intracellular transport of drugs and may participate in chemoresistance of SCLC by sequestration, rather than mediating cellular uptake. Since satraplatin failed to improve survival in SCLC patients, the relation of OATP5A1 expression to clinical drug resistance and its use as marker of chemoresistance should be further investigated.
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spelling pubmed-37919162013-10-31 Organic Anion Transporting Polypeptide 5A1 (OATP5A1) in Small Cell Lung Cancer (SCLC) Cells: Possible Involvement in Chemoresistance to Satraplatin Olszewski-Hamilton, U. Svoboda, M. Thalhammer, T. Buxhofer-Ausch, V. Geissler, K. Hamilton, G. Biomark Cancer Original Research BACKGROUND: The role of organic anion transporting polypeptide 5A1 (OATP5A1) a member of a family of drug transporters that mediate cellular uptake of drugs has not been characterized so far. METHODS: Gene expression levels of OATP5A1 in small cell lung cancer (SCLC) cell lines were determined by real-time qPCR and chemosensitivity of HEK-293-SLCO5A1-transfected cells to satraplatin in MTT assays. RESULTS: Significant expression of this transporter was found at the mRNA level, primarily in drug-resistant SCLC cells, and SLCO5A1-transfected HEK-293 cells showed higher resistance to satraplatin. OATP5A1 is found preferentially in cytoplasmic membranes of tumor cells, including SCLC. CONCLUSIONS: OATP5A1 seems to effect intracellular transport of drugs and may participate in chemoresistance of SCLC by sequestration, rather than mediating cellular uptake. Since satraplatin failed to improve survival in SCLC patients, the relation of OATP5A1 expression to clinical drug resistance and its use as marker of chemoresistance should be further investigated. Libertas Academica 2011-06-07 /pmc/articles/PMC3791916/ /pubmed/24179389 http://dx.doi.org/10.4137/BIC.S7151 Text en © 2011 the author(s), publisher and licensee Libertas Academica Ltd. This is an open access article. Unrestricted non-commercial use is permitted provided the original work is properly cited.
spellingShingle Original Research
Olszewski-Hamilton, U.
Svoboda, M.
Thalhammer, T.
Buxhofer-Ausch, V.
Geissler, K.
Hamilton, G.
Organic Anion Transporting Polypeptide 5A1 (OATP5A1) in Small Cell Lung Cancer (SCLC) Cells: Possible Involvement in Chemoresistance to Satraplatin
title Organic Anion Transporting Polypeptide 5A1 (OATP5A1) in Small Cell Lung Cancer (SCLC) Cells: Possible Involvement in Chemoresistance to Satraplatin
title_full Organic Anion Transporting Polypeptide 5A1 (OATP5A1) in Small Cell Lung Cancer (SCLC) Cells: Possible Involvement in Chemoresistance to Satraplatin
title_fullStr Organic Anion Transporting Polypeptide 5A1 (OATP5A1) in Small Cell Lung Cancer (SCLC) Cells: Possible Involvement in Chemoresistance to Satraplatin
title_full_unstemmed Organic Anion Transporting Polypeptide 5A1 (OATP5A1) in Small Cell Lung Cancer (SCLC) Cells: Possible Involvement in Chemoresistance to Satraplatin
title_short Organic Anion Transporting Polypeptide 5A1 (OATP5A1) in Small Cell Lung Cancer (SCLC) Cells: Possible Involvement in Chemoresistance to Satraplatin
title_sort organic anion transporting polypeptide 5a1 (oatp5a1) in small cell lung cancer (sclc) cells: possible involvement in chemoresistance to satraplatin
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3791916/
https://www.ncbi.nlm.nih.gov/pubmed/24179389
http://dx.doi.org/10.4137/BIC.S7151
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