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Biotic and Abiotic Soil Properties Influence Survival of Listeria monocytogenes in Soil

Listeria monocytogenes is a food-borne pathogen responsible for the potentially fatal disease listeriosis and terrestrial ecosystems have been hypothesized to be its natural reservoir. Therefore, identifying the key edaphic factors that influence its survival in soil is critical. We measured the sur...

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Autores principales: Locatelli, Aude, Spor, Aymé, Jolivet, Claudy, Piveteau, Pascal, Hartmann, Alain
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3792134/
https://www.ncbi.nlm.nih.gov/pubmed/24116083
http://dx.doi.org/10.1371/journal.pone.0075969
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author Locatelli, Aude
Spor, Aymé
Jolivet, Claudy
Piveteau, Pascal
Hartmann, Alain
author_facet Locatelli, Aude
Spor, Aymé
Jolivet, Claudy
Piveteau, Pascal
Hartmann, Alain
author_sort Locatelli, Aude
collection PubMed
description Listeria monocytogenes is a food-borne pathogen responsible for the potentially fatal disease listeriosis and terrestrial ecosystems have been hypothesized to be its natural reservoir. Therefore, identifying the key edaphic factors that influence its survival in soil is critical. We measured the survival of L. monocytogenes in a set of 100 soil samples belonging to the French Soil Quality Monitoring Network. This soil collection is meant to be representative of the pedology and land use of the whole French territory. The population of L. monocytogenes in inoculated microcosms was enumerated by plate count after 7, 14 and 84 days of incubation. Analysis of survival profiles showed that L. monocytogenes was able to survive up to 84 days in 71% of the soils tested, in the other soils (29%) only a short-term survival (up to 7 to 14 days) was observed. Using variance partitioning techniques, we showed that about 65% of the short-term survival ratio of L. monocytogenes in soils was explained by the soil chemical properties, amongst which the basic cation saturation ratio seems to be the main driver. On the other hand, while explaining a lower amount of survival ratio variance (11%), soil texture and especially clay content was the main driver of long-term survival of L. monocytogenes in soils. In order to assess the effect of the endogenous soils microbiota on L. monocytogenes survival, sterilized versus non-sterilized soils microcosms were compared in a subset of 9 soils. We found that the endogenous soil microbiota could limit L. monocytogenes survival especially when soil pH was greater than 7, whereas in acidic soils, survival ratios in sterilized and unsterilized microcosms were not statistically different. These results point out the critical role played by both the endogenous microbiota and the soil physic-chemical properties in determining the survival of L. monocytogenes in soils.
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spelling pubmed-37921342013-10-10 Biotic and Abiotic Soil Properties Influence Survival of Listeria monocytogenes in Soil Locatelli, Aude Spor, Aymé Jolivet, Claudy Piveteau, Pascal Hartmann, Alain PLoS One Research Article Listeria monocytogenes is a food-borne pathogen responsible for the potentially fatal disease listeriosis and terrestrial ecosystems have been hypothesized to be its natural reservoir. Therefore, identifying the key edaphic factors that influence its survival in soil is critical. We measured the survival of L. monocytogenes in a set of 100 soil samples belonging to the French Soil Quality Monitoring Network. This soil collection is meant to be representative of the pedology and land use of the whole French territory. The population of L. monocytogenes in inoculated microcosms was enumerated by plate count after 7, 14 and 84 days of incubation. Analysis of survival profiles showed that L. monocytogenes was able to survive up to 84 days in 71% of the soils tested, in the other soils (29%) only a short-term survival (up to 7 to 14 days) was observed. Using variance partitioning techniques, we showed that about 65% of the short-term survival ratio of L. monocytogenes in soils was explained by the soil chemical properties, amongst which the basic cation saturation ratio seems to be the main driver. On the other hand, while explaining a lower amount of survival ratio variance (11%), soil texture and especially clay content was the main driver of long-term survival of L. monocytogenes in soils. In order to assess the effect of the endogenous soils microbiota on L. monocytogenes survival, sterilized versus non-sterilized soils microcosms were compared in a subset of 9 soils. We found that the endogenous soil microbiota could limit L. monocytogenes survival especially when soil pH was greater than 7, whereas in acidic soils, survival ratios in sterilized and unsterilized microcosms were not statistically different. These results point out the critical role played by both the endogenous microbiota and the soil physic-chemical properties in determining the survival of L. monocytogenes in soils. Public Library of Science 2013-10-07 /pmc/articles/PMC3792134/ /pubmed/24116083 http://dx.doi.org/10.1371/journal.pone.0075969 Text en © 2013 Locatelli et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Locatelli, Aude
Spor, Aymé
Jolivet, Claudy
Piveteau, Pascal
Hartmann, Alain
Biotic and Abiotic Soil Properties Influence Survival of Listeria monocytogenes in Soil
title Biotic and Abiotic Soil Properties Influence Survival of Listeria monocytogenes in Soil
title_full Biotic and Abiotic Soil Properties Influence Survival of Listeria monocytogenes in Soil
title_fullStr Biotic and Abiotic Soil Properties Influence Survival of Listeria monocytogenes in Soil
title_full_unstemmed Biotic and Abiotic Soil Properties Influence Survival of Listeria monocytogenes in Soil
title_short Biotic and Abiotic Soil Properties Influence Survival of Listeria monocytogenes in Soil
title_sort biotic and abiotic soil properties influence survival of listeria monocytogenes in soil
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3792134/
https://www.ncbi.nlm.nih.gov/pubmed/24116083
http://dx.doi.org/10.1371/journal.pone.0075969
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