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Tetrazolium Violet Induced Apoptosis and Cell Cycle Arrest in Human Lung Cancer A549 Cells

Tetrazolium violet is a tetrazolium salt and has been proposed as an antitumor agent. In this study, we reported for the first time that tetrazolium violet not only inhibited human lung cancer A549 cell proliferation but also induced apoptosis and blocked cell cycle progression in the G1 phase. The...

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Autores principales: Zhang, Xiao-Hong, Zhang, Nan, Lu, Jian-Mei, Kong, Qing-Zhong, Zhao, Yun-Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Applied Pharmacology 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3792215/
https://www.ncbi.nlm.nih.gov/pubmed/24116292
http://dx.doi.org/10.4062/biomolther.2012.20.2.177
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author Zhang, Xiao-Hong
Zhang, Nan
Lu, Jian-Mei
Kong, Qing-Zhong
Zhao, Yun-Feng
author_facet Zhang, Xiao-Hong
Zhang, Nan
Lu, Jian-Mei
Kong, Qing-Zhong
Zhao, Yun-Feng
author_sort Zhang, Xiao-Hong
collection PubMed
description Tetrazolium violet is a tetrazolium salt and has been proposed as an antitumor agent. In this study, we reported for the first time that tetrazolium violet not only inhibited human lung cancer A549 cell proliferation but also induced apoptosis and blocked cell cycle progression in the G1 phase. The results showed that tetrazolium violet significantly decreased the viability of A549 cells at 5-15 μM. Tetrazolium violet -induced apoptosis in A549 cells was confirmed by H33258 staining assay. In A549, tetrazolium violet blocked the progression of the cell cycle at G1 phase by inducing p53 expression and further up-regulating p21/WAF1 expression. In addition, an enhancement in Fas/APO-1 and its two forms of ligands, membrane-bound Fas ligand (mFasL) and soluble Fas ligand (sFasL), as well as caspase, were responsible for the apoptotic effect induced by tetrazolium violet. The conclusion of this study is that tetrazolium violet induced p53 expression which caused cell cycle arrest and apoptosis. These findings suggest that tetrazolium violet has strong potential for development as an agent for treatment lung cancer.
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spelling pubmed-37922152013-10-10 Tetrazolium Violet Induced Apoptosis and Cell Cycle Arrest in Human Lung Cancer A549 Cells Zhang, Xiao-Hong Zhang, Nan Lu, Jian-Mei Kong, Qing-Zhong Zhao, Yun-Feng Biomol Ther (Seoul) Articles Tetrazolium violet is a tetrazolium salt and has been proposed as an antitumor agent. In this study, we reported for the first time that tetrazolium violet not only inhibited human lung cancer A549 cell proliferation but also induced apoptosis and blocked cell cycle progression in the G1 phase. The results showed that tetrazolium violet significantly decreased the viability of A549 cells at 5-15 μM. Tetrazolium violet -induced apoptosis in A549 cells was confirmed by H33258 staining assay. In A549, tetrazolium violet blocked the progression of the cell cycle at G1 phase by inducing p53 expression and further up-regulating p21/WAF1 expression. In addition, an enhancement in Fas/APO-1 and its two forms of ligands, membrane-bound Fas ligand (mFasL) and soluble Fas ligand (sFasL), as well as caspase, were responsible for the apoptotic effect induced by tetrazolium violet. The conclusion of this study is that tetrazolium violet induced p53 expression which caused cell cycle arrest and apoptosis. These findings suggest that tetrazolium violet has strong potential for development as an agent for treatment lung cancer. The Korean Society of Applied Pharmacology 2012-03 /pmc/articles/PMC3792215/ /pubmed/24116292 http://dx.doi.org/10.4062/biomolther.2012.20.2.177 Text en Copyright ©2012, The Korean Society of Applied Pharmacology http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles
Zhang, Xiao-Hong
Zhang, Nan
Lu, Jian-Mei
Kong, Qing-Zhong
Zhao, Yun-Feng
Tetrazolium Violet Induced Apoptosis and Cell Cycle Arrest in Human Lung Cancer A549 Cells
title Tetrazolium Violet Induced Apoptosis and Cell Cycle Arrest in Human Lung Cancer A549 Cells
title_full Tetrazolium Violet Induced Apoptosis and Cell Cycle Arrest in Human Lung Cancer A549 Cells
title_fullStr Tetrazolium Violet Induced Apoptosis and Cell Cycle Arrest in Human Lung Cancer A549 Cells
title_full_unstemmed Tetrazolium Violet Induced Apoptosis and Cell Cycle Arrest in Human Lung Cancer A549 Cells
title_short Tetrazolium Violet Induced Apoptosis and Cell Cycle Arrest in Human Lung Cancer A549 Cells
title_sort tetrazolium violet induced apoptosis and cell cycle arrest in human lung cancer a549 cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3792215/
https://www.ncbi.nlm.nih.gov/pubmed/24116292
http://dx.doi.org/10.4062/biomolther.2012.20.2.177
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