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An Animal Explant Model for the Study of Human Cutaneous Squamous Cell Carcinoma
We established a human tissue explant model to facilitate study of cutaneous squamous cell carcinoma. We accomplished this by implanting debulked SCC, from surgical discard, into nude rats. Human SCC remained viable and continued to proliferate for at least 4 weeks and showed evidence of neovascular...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3792940/ https://www.ncbi.nlm.nih.gov/pubmed/24116092 http://dx.doi.org/10.1371/journal.pone.0076156 |
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author | Belkin, Daniel A. Chen, Jie Mo, Jonathan L. Rosoff, James S. Goldenberg, Sagit Poppas, Dix P. Krueger, James G. Herschman, Miriam Mitsui, Hiroshi Felsen, Diane Carucci, John A. |
author_facet | Belkin, Daniel A. Chen, Jie Mo, Jonathan L. Rosoff, James S. Goldenberg, Sagit Poppas, Dix P. Krueger, James G. Herschman, Miriam Mitsui, Hiroshi Felsen, Diane Carucci, John A. |
author_sort | Belkin, Daniel A. |
collection | PubMed |
description | We established a human tissue explant model to facilitate study of cutaneous squamous cell carcinoma. We accomplished this by implanting debulked SCC, from surgical discard, into nude rats. Human SCC remained viable and continued to proliferate for at least 4 weeks and showed evidence of neovascularization. At 4 weeks, SCC implants showed a trend toward increased PCNA positive cells compared to fresh SCC cells/mm(2) tissue) supporting continued proliferation throughout engraftment. Von Willebrand's Factor (VWF) positive cells were found within implants and likely represented rat vessel neovascularization. Human Langerhans' (Langerin+) cells, but no T cells (CD3+, CD8+, FoxP3+), macrophages (CD163), or NK cells (NKp46), were present in SCC implants at 4 weeks. These findings support the possibility that LCs fail to migrate from cutaneous SCC and thus contribute to lack of effective antitumor response. Our findings also provide a novel model system for further study of primary cutaneous SCC. |
format | Online Article Text |
id | pubmed-3792940 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-37929402013-10-10 An Animal Explant Model for the Study of Human Cutaneous Squamous Cell Carcinoma Belkin, Daniel A. Chen, Jie Mo, Jonathan L. Rosoff, James S. Goldenberg, Sagit Poppas, Dix P. Krueger, James G. Herschman, Miriam Mitsui, Hiroshi Felsen, Diane Carucci, John A. PLoS One Research Article We established a human tissue explant model to facilitate study of cutaneous squamous cell carcinoma. We accomplished this by implanting debulked SCC, from surgical discard, into nude rats. Human SCC remained viable and continued to proliferate for at least 4 weeks and showed evidence of neovascularization. At 4 weeks, SCC implants showed a trend toward increased PCNA positive cells compared to fresh SCC cells/mm(2) tissue) supporting continued proliferation throughout engraftment. Von Willebrand's Factor (VWF) positive cells were found within implants and likely represented rat vessel neovascularization. Human Langerhans' (Langerin+) cells, but no T cells (CD3+, CD8+, FoxP3+), macrophages (CD163), or NK cells (NKp46), were present in SCC implants at 4 weeks. These findings support the possibility that LCs fail to migrate from cutaneous SCC and thus contribute to lack of effective antitumor response. Our findings also provide a novel model system for further study of primary cutaneous SCC. Public Library of Science 2013-10-08 /pmc/articles/PMC3792940/ /pubmed/24116092 http://dx.doi.org/10.1371/journal.pone.0076156 Text en © 2013 Belkin et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Belkin, Daniel A. Chen, Jie Mo, Jonathan L. Rosoff, James S. Goldenberg, Sagit Poppas, Dix P. Krueger, James G. Herschman, Miriam Mitsui, Hiroshi Felsen, Diane Carucci, John A. An Animal Explant Model for the Study of Human Cutaneous Squamous Cell Carcinoma |
title | An Animal Explant Model for the Study of Human Cutaneous Squamous Cell Carcinoma |
title_full | An Animal Explant Model for the Study of Human Cutaneous Squamous Cell Carcinoma |
title_fullStr | An Animal Explant Model for the Study of Human Cutaneous Squamous Cell Carcinoma |
title_full_unstemmed | An Animal Explant Model for the Study of Human Cutaneous Squamous Cell Carcinoma |
title_short | An Animal Explant Model for the Study of Human Cutaneous Squamous Cell Carcinoma |
title_sort | animal explant model for the study of human cutaneous squamous cell carcinoma |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3792940/ https://www.ncbi.nlm.nih.gov/pubmed/24116092 http://dx.doi.org/10.1371/journal.pone.0076156 |
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