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The Tight-Junction Protein Claudin-6 Induces Epithelial Differentiation from Mouse F9 and Embryonic Stem Cells

During epithelialization, cell adhesions and polarity must be established to maintain tissue assemblies and separate the biological compartments in the body. However, the molecular basis of epithelial morphogenesis, in particular, a role of cell adhesion molecules in epithelial differentiation from...

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Autores principales: Sugimoto, Kotaro, Ichikawa-Tomikawa, Naoki, Satohisa, Seiro, Akashi, Yushi, Kanai, Risa, Saito, Tsuyoshi, Sawada, Norimasa, Chiba, Hideki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3792957/
https://www.ncbi.nlm.nih.gov/pubmed/24116027
http://dx.doi.org/10.1371/journal.pone.0075106
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author Sugimoto, Kotaro
Ichikawa-Tomikawa, Naoki
Satohisa, Seiro
Akashi, Yushi
Kanai, Risa
Saito, Tsuyoshi
Sawada, Norimasa
Chiba, Hideki
author_facet Sugimoto, Kotaro
Ichikawa-Tomikawa, Naoki
Satohisa, Seiro
Akashi, Yushi
Kanai, Risa
Saito, Tsuyoshi
Sawada, Norimasa
Chiba, Hideki
author_sort Sugimoto, Kotaro
collection PubMed
description During epithelialization, cell adhesions and polarity must be established to maintain tissue assemblies and separate the biological compartments in the body. However, the molecular basis of epithelial morphogenesis, in particular, a role of cell adhesion molecules in epithelial differentiation from stem cells, remains unclear. Here, we show that the stable and conditional expression of a tight-junction protein, claudin-6 (Cldn6), triggers epithelial morphogenesis in mouse F9 stem cells. We also demonstrate that Cldn6 induces the expression of other tight-junction and microvillus molecules including Cldn7, occludin, ZO-1α+, and ezrin/radixin/moesin-binding phosphoprotein50. These events were inhibited by attenuation of Cldn6 using RNA interference or the C-terminal half of Clostridium Perfringens enterotoxin. Furthermore, similar results were obtained in mouse embryonic stem cells. Thus, we have uncovered that the Cldn6 functions as a novel cue to induce epithelial differentiation.
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spelling pubmed-37929572013-10-10 The Tight-Junction Protein Claudin-6 Induces Epithelial Differentiation from Mouse F9 and Embryonic Stem Cells Sugimoto, Kotaro Ichikawa-Tomikawa, Naoki Satohisa, Seiro Akashi, Yushi Kanai, Risa Saito, Tsuyoshi Sawada, Norimasa Chiba, Hideki PLoS One Research Article During epithelialization, cell adhesions and polarity must be established to maintain tissue assemblies and separate the biological compartments in the body. However, the molecular basis of epithelial morphogenesis, in particular, a role of cell adhesion molecules in epithelial differentiation from stem cells, remains unclear. Here, we show that the stable and conditional expression of a tight-junction protein, claudin-6 (Cldn6), triggers epithelial morphogenesis in mouse F9 stem cells. We also demonstrate that Cldn6 induces the expression of other tight-junction and microvillus molecules including Cldn7, occludin, ZO-1α+, and ezrin/radixin/moesin-binding phosphoprotein50. These events were inhibited by attenuation of Cldn6 using RNA interference or the C-terminal half of Clostridium Perfringens enterotoxin. Furthermore, similar results were obtained in mouse embryonic stem cells. Thus, we have uncovered that the Cldn6 functions as a novel cue to induce epithelial differentiation. Public Library of Science 2013-10-08 /pmc/articles/PMC3792957/ /pubmed/24116027 http://dx.doi.org/10.1371/journal.pone.0075106 Text en © 2013 Sugimoto et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Sugimoto, Kotaro
Ichikawa-Tomikawa, Naoki
Satohisa, Seiro
Akashi, Yushi
Kanai, Risa
Saito, Tsuyoshi
Sawada, Norimasa
Chiba, Hideki
The Tight-Junction Protein Claudin-6 Induces Epithelial Differentiation from Mouse F9 and Embryonic Stem Cells
title The Tight-Junction Protein Claudin-6 Induces Epithelial Differentiation from Mouse F9 and Embryonic Stem Cells
title_full The Tight-Junction Protein Claudin-6 Induces Epithelial Differentiation from Mouse F9 and Embryonic Stem Cells
title_fullStr The Tight-Junction Protein Claudin-6 Induces Epithelial Differentiation from Mouse F9 and Embryonic Stem Cells
title_full_unstemmed The Tight-Junction Protein Claudin-6 Induces Epithelial Differentiation from Mouse F9 and Embryonic Stem Cells
title_short The Tight-Junction Protein Claudin-6 Induces Epithelial Differentiation from Mouse F9 and Embryonic Stem Cells
title_sort tight-junction protein claudin-6 induces epithelial differentiation from mouse f9 and embryonic stem cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3792957/
https://www.ncbi.nlm.nih.gov/pubmed/24116027
http://dx.doi.org/10.1371/journal.pone.0075106
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