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The Impact of Helicobacter pylori Infection on the Gastric Microbiota of the Rhesus Macaque
Helicobacter pylori colonization is highly prevalent among humans and causes significant gastric disease in a subset of those infected. When present, this bacterium dominates the gastric microbiota of humans and induces antimicrobial responses in the host. Since the microbial context of H. pylori co...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3792980/ https://www.ncbi.nlm.nih.gov/pubmed/24116104 http://dx.doi.org/10.1371/journal.pone.0076375 |
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author | Martin, Miriam E. Bhatnagar, Srijak George, Michael D. Paster, Bruce J. Canfield, Don R. Eisen, Jonathan A. Solnick, Jay V. |
author_facet | Martin, Miriam E. Bhatnagar, Srijak George, Michael D. Paster, Bruce J. Canfield, Don R. Eisen, Jonathan A. Solnick, Jay V. |
author_sort | Martin, Miriam E. |
collection | PubMed |
description | Helicobacter pylori colonization is highly prevalent among humans and causes significant gastric disease in a subset of those infected. When present, this bacterium dominates the gastric microbiota of humans and induces antimicrobial responses in the host. Since the microbial context of H. pylori colonization influences the disease outcome in a mouse model, we sought to assess the impact of H. pylori challenge upon the pre-existing gastric microbial community members in the rhesus macaque model. Deep sequencing of the bacterial 16S rRNA gene identified a community profile of 221 phylotypes that was distinct from that of the rhesus macaque distal gut and mouth, although there were taxa in common. High proportions of both H. pylori and H. suis were observed in the post-challenge libraries, but at a given time, only one Helicobacter species was dominant. However, the relative abundance of non-Helicobacter taxa was not significantly different before and after challenge with H. pylori. These results suggest that while different gastric species may show competitive exclusion in the gastric niche, the rhesus gastric microbial community is largely stable despite immune and physiological changes due to H. pylori infection. |
format | Online Article Text |
id | pubmed-3792980 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-37929802013-10-10 The Impact of Helicobacter pylori Infection on the Gastric Microbiota of the Rhesus Macaque Martin, Miriam E. Bhatnagar, Srijak George, Michael D. Paster, Bruce J. Canfield, Don R. Eisen, Jonathan A. Solnick, Jay V. PLoS One Research Article Helicobacter pylori colonization is highly prevalent among humans and causes significant gastric disease in a subset of those infected. When present, this bacterium dominates the gastric microbiota of humans and induces antimicrobial responses in the host. Since the microbial context of H. pylori colonization influences the disease outcome in a mouse model, we sought to assess the impact of H. pylori challenge upon the pre-existing gastric microbial community members in the rhesus macaque model. Deep sequencing of the bacterial 16S rRNA gene identified a community profile of 221 phylotypes that was distinct from that of the rhesus macaque distal gut and mouth, although there were taxa in common. High proportions of both H. pylori and H. suis were observed in the post-challenge libraries, but at a given time, only one Helicobacter species was dominant. However, the relative abundance of non-Helicobacter taxa was not significantly different before and after challenge with H. pylori. These results suggest that while different gastric species may show competitive exclusion in the gastric niche, the rhesus gastric microbial community is largely stable despite immune and physiological changes due to H. pylori infection. Public Library of Science 2013-10-08 /pmc/articles/PMC3792980/ /pubmed/24116104 http://dx.doi.org/10.1371/journal.pone.0076375 Text en © 2013 Martin et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Martin, Miriam E. Bhatnagar, Srijak George, Michael D. Paster, Bruce J. Canfield, Don R. Eisen, Jonathan A. Solnick, Jay V. The Impact of Helicobacter pylori Infection on the Gastric Microbiota of the Rhesus Macaque |
title | The Impact of Helicobacter pylori Infection on the Gastric Microbiota of the Rhesus Macaque |
title_full | The Impact of Helicobacter pylori Infection on the Gastric Microbiota of the Rhesus Macaque |
title_fullStr | The Impact of Helicobacter pylori Infection on the Gastric Microbiota of the Rhesus Macaque |
title_full_unstemmed | The Impact of Helicobacter pylori Infection on the Gastric Microbiota of the Rhesus Macaque |
title_short | The Impact of Helicobacter pylori Infection on the Gastric Microbiota of the Rhesus Macaque |
title_sort | impact of helicobacter pylori infection on the gastric microbiota of the rhesus macaque |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3792980/ https://www.ncbi.nlm.nih.gov/pubmed/24116104 http://dx.doi.org/10.1371/journal.pone.0076375 |
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