Identification and Characterization of a Cold-Active Phthalate Esters Hydrolase by Screening a Metagenomic Library Derived from Biofilms of a Wastewater Treatment Plant

A cold-active phthalate esters hydrolase gene (designated dphB) was identified through functional screening of a metagenomic library derived from biofilms of a wastewater treatment plant. The enzyme specifically catalyzed the hydrolysis of dipropyl phthalate, dibutyl phthalate, and dipentyl phthalat...

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Detalles Bibliográficos
Autores principales: Jiao, Yiying, Chen, Xu, Wang, Xin, Liao, Xuewei, Xiao, Lin, Miao, Aijun, Wu, Jun, Yang, Liuyan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3792999/
https://www.ncbi.nlm.nih.gov/pubmed/24116085
http://dx.doi.org/10.1371/journal.pone.0075977
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author Jiao, Yiying
Chen, Xu
Wang, Xin
Liao, Xuewei
Xiao, Lin
Miao, Aijun
Wu, Jun
Yang, Liuyan
author_facet Jiao, Yiying
Chen, Xu
Wang, Xin
Liao, Xuewei
Xiao, Lin
Miao, Aijun
Wu, Jun
Yang, Liuyan
author_sort Jiao, Yiying
collection PubMed
description A cold-active phthalate esters hydrolase gene (designated dphB) was identified through functional screening of a metagenomic library derived from biofilms of a wastewater treatment plant. The enzyme specifically catalyzed the hydrolysis of dipropyl phthalate, dibutyl phthalate, and dipentyl phthalate to the corresponding monoalkyl phthalate esters at low temperatures. The catalytic triad residues of DphB were proposed to be Ser159, Asp251, and His281.
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spelling pubmed-37929992013-10-10 Identification and Characterization of a Cold-Active Phthalate Esters Hydrolase by Screening a Metagenomic Library Derived from Biofilms of a Wastewater Treatment Plant Jiao, Yiying Chen, Xu Wang, Xin Liao, Xuewei Xiao, Lin Miao, Aijun Wu, Jun Yang, Liuyan PLoS One Research Article A cold-active phthalate esters hydrolase gene (designated dphB) was identified through functional screening of a metagenomic library derived from biofilms of a wastewater treatment plant. The enzyme specifically catalyzed the hydrolysis of dipropyl phthalate, dibutyl phthalate, and dipentyl phthalate to the corresponding monoalkyl phthalate esters at low temperatures. The catalytic triad residues of DphB were proposed to be Ser159, Asp251, and His281. Public Library of Science 2013-10-08 /pmc/articles/PMC3792999/ /pubmed/24116085 http://dx.doi.org/10.1371/journal.pone.0075977 Text en © 2013 Jiao et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Jiao, Yiying
Chen, Xu
Wang, Xin
Liao, Xuewei
Xiao, Lin
Miao, Aijun
Wu, Jun
Yang, Liuyan
Identification and Characterization of a Cold-Active Phthalate Esters Hydrolase by Screening a Metagenomic Library Derived from Biofilms of a Wastewater Treatment Plant
title Identification and Characterization of a Cold-Active Phthalate Esters Hydrolase by Screening a Metagenomic Library Derived from Biofilms of a Wastewater Treatment Plant
title_full Identification and Characterization of a Cold-Active Phthalate Esters Hydrolase by Screening a Metagenomic Library Derived from Biofilms of a Wastewater Treatment Plant
title_fullStr Identification and Characterization of a Cold-Active Phthalate Esters Hydrolase by Screening a Metagenomic Library Derived from Biofilms of a Wastewater Treatment Plant
title_full_unstemmed Identification and Characterization of a Cold-Active Phthalate Esters Hydrolase by Screening a Metagenomic Library Derived from Biofilms of a Wastewater Treatment Plant
title_short Identification and Characterization of a Cold-Active Phthalate Esters Hydrolase by Screening a Metagenomic Library Derived from Biofilms of a Wastewater Treatment Plant
title_sort identification and characterization of a cold-active phthalate esters hydrolase by screening a metagenomic library derived from biofilms of a wastewater treatment plant
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3792999/
https://www.ncbi.nlm.nih.gov/pubmed/24116085
http://dx.doi.org/10.1371/journal.pone.0075977
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