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Monoclonal antibody 10A5 recognizes an antigen unique to the water-insoluble 25/45 membrane fraction of the rat ocular lens

The water-insoluble 25/45 fraction and non-sedimenting membrane fraction (NSMF) are two membrane preparations isolated from the ocular lens. The fractions are postulated to represent distinct subdomains of the lens with unique functions. However, attempts to distinguish between the two fractions by...

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Autores principales: Whitman, Joseph K, Alviar, Abigail F, Fleschner, Charles R, Stuart, Melissa K
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3793078/
https://www.ncbi.nlm.nih.gov/pubmed/24109564
http://dx.doi.org/10.1186/2193-1801-2-500
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author Whitman, Joseph K
Alviar, Abigail F
Fleschner, Charles R
Stuart, Melissa K
author_facet Whitman, Joseph K
Alviar, Abigail F
Fleschner, Charles R
Stuart, Melissa K
author_sort Whitman, Joseph K
collection PubMed
description The water-insoluble 25/45 fraction and non-sedimenting membrane fraction (NSMF) are two membrane preparations isolated from the ocular lens. The fractions are postulated to represent distinct subdomains of the lens with unique functions. However, attempts to distinguish between the two fractions by detecting proteins present in one fraction but absent from other have been unsuccessful. In this study, we exploited the ability of the mouse immune system to detect antigenic differences between the 25/45 fraction and NSMF isolated from the lenses of 20-day-old rats. We generated a monoclonal antibody (MAb 10A5) that reacts with a ganglioside-like antigen that is present in the 25/45 fraction but absent from the NSMF. Restriction of the antigen to the 25/45 fraction in 20-day-old animals supports the hypothesis that the 25/45 fraction and NSMF represent different subdomains within the ocular lens. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/2193-1801-2-500) contains supplementary material, which is available to authorized users.
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spelling pubmed-37930782013-10-09 Monoclonal antibody 10A5 recognizes an antigen unique to the water-insoluble 25/45 membrane fraction of the rat ocular lens Whitman, Joseph K Alviar, Abigail F Fleschner, Charles R Stuart, Melissa K Springerplus Research The water-insoluble 25/45 fraction and non-sedimenting membrane fraction (NSMF) are two membrane preparations isolated from the ocular lens. The fractions are postulated to represent distinct subdomains of the lens with unique functions. However, attempts to distinguish between the two fractions by detecting proteins present in one fraction but absent from other have been unsuccessful. In this study, we exploited the ability of the mouse immune system to detect antigenic differences between the 25/45 fraction and NSMF isolated from the lenses of 20-day-old rats. We generated a monoclonal antibody (MAb 10A5) that reacts with a ganglioside-like antigen that is present in the 25/45 fraction but absent from the NSMF. Restriction of the antigen to the 25/45 fraction in 20-day-old animals supports the hypothesis that the 25/45 fraction and NSMF represent different subdomains within the ocular lens. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/2193-1801-2-500) contains supplementary material, which is available to authorized users. Springer International Publishing 2013-10-02 /pmc/articles/PMC3793078/ /pubmed/24109564 http://dx.doi.org/10.1186/2193-1801-2-500 Text en © Whitman et al.; licensee Springer. 2013 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Whitman, Joseph K
Alviar, Abigail F
Fleschner, Charles R
Stuart, Melissa K
Monoclonal antibody 10A5 recognizes an antigen unique to the water-insoluble 25/45 membrane fraction of the rat ocular lens
title Monoclonal antibody 10A5 recognizes an antigen unique to the water-insoluble 25/45 membrane fraction of the rat ocular lens
title_full Monoclonal antibody 10A5 recognizes an antigen unique to the water-insoluble 25/45 membrane fraction of the rat ocular lens
title_fullStr Monoclonal antibody 10A5 recognizes an antigen unique to the water-insoluble 25/45 membrane fraction of the rat ocular lens
title_full_unstemmed Monoclonal antibody 10A5 recognizes an antigen unique to the water-insoluble 25/45 membrane fraction of the rat ocular lens
title_short Monoclonal antibody 10A5 recognizes an antigen unique to the water-insoluble 25/45 membrane fraction of the rat ocular lens
title_sort monoclonal antibody 10a5 recognizes an antigen unique to the water-insoluble 25/45 membrane fraction of the rat ocular lens
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3793078/
https://www.ncbi.nlm.nih.gov/pubmed/24109564
http://dx.doi.org/10.1186/2193-1801-2-500
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