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Chemoprotective potential of Coccinia indica against cyclophosphamide-induced toxicity
OBJECTIVE: Although cyclophosphamide (CP), an alkylating agent, is used in the treatment of cancer owing to its broad-spectrum efficacy, its metabolites exhibit severe undesired toxicities in normal cells. The present study was aimed to investigate the chemoprotective potential of Coccinia indica ag...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Medknow Publications & Media Pvt Ltd
2013
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3793523/ https://www.ncbi.nlm.nih.gov/pubmed/24130387 http://dx.doi.org/10.4103/0253-7613.117783 |
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author | Nitharwal, Ramesh K Patel, Hasit Karchuli, Manvendra Singh Ugale, Rajesh Ramesh |
author_facet | Nitharwal, Ramesh K Patel, Hasit Karchuli, Manvendra Singh Ugale, Rajesh Ramesh |
author_sort | Nitharwal, Ramesh K |
collection | PubMed |
description | OBJECTIVE: Although cyclophosphamide (CP), an alkylating agent, is used in the treatment of cancer owing to its broad-spectrum efficacy, its metabolites exhibit severe undesired toxicities in normal cells. The present study was aimed to investigate the chemoprotective potential of Coccinia indica against CP-induced oxidative stress, genotoxicity, and hepatotoxicity. MATERIALS AND METHODS: Rodents were orally pre-treated with Coccinia indica extract (200, 400, and 600 mg/kg) for five consecutive days. On 5th day, these animals were injected with CP (50 mg/kg i.p) and sacrificed after 24 hrs. for the evaluation of oxidative stress, hepatotoxicity, micronucleus formation, and chromosomal aberrations. RESULTS: We found that the CP significantly increased malondialdehyde (MDA) and decreased catalase and glutathione (GSH) levels in brain, and it was significantly reversed by Coccinia indica extract (400 and 600 mg/kg). Further, pre-treatment with Coccinia indica extract (200, 400, 600 mg/kg) significantly and dose-dependently reduced micronuclei formation and incidence of aberrant cells. We also found that the CP-induced increase in the serum biomarker enzymes like alkaline phosphatase (ALP), alkaline aminotransferase (ALT), and aspartate aminotransferase (AST) were significantly reduced by Coccinia indica extract. CONCLUSION: Thus, the present results indicate the protective effect of Coccinia indica extract against CP-induced oxidative stress, genotoxicity, as well as hepatotoxicity. |
format | Online Article Text |
id | pubmed-3793523 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-37935232013-10-15 Chemoprotective potential of Coccinia indica against cyclophosphamide-induced toxicity Nitharwal, Ramesh K Patel, Hasit Karchuli, Manvendra Singh Ugale, Rajesh Ramesh Indian J Pharmacol Research Article OBJECTIVE: Although cyclophosphamide (CP), an alkylating agent, is used in the treatment of cancer owing to its broad-spectrum efficacy, its metabolites exhibit severe undesired toxicities in normal cells. The present study was aimed to investigate the chemoprotective potential of Coccinia indica against CP-induced oxidative stress, genotoxicity, and hepatotoxicity. MATERIALS AND METHODS: Rodents were orally pre-treated with Coccinia indica extract (200, 400, and 600 mg/kg) for five consecutive days. On 5th day, these animals were injected with CP (50 mg/kg i.p) and sacrificed after 24 hrs. for the evaluation of oxidative stress, hepatotoxicity, micronucleus formation, and chromosomal aberrations. RESULTS: We found that the CP significantly increased malondialdehyde (MDA) and decreased catalase and glutathione (GSH) levels in brain, and it was significantly reversed by Coccinia indica extract (400 and 600 mg/kg). Further, pre-treatment with Coccinia indica extract (200, 400, 600 mg/kg) significantly and dose-dependently reduced micronuclei formation and incidence of aberrant cells. We also found that the CP-induced increase in the serum biomarker enzymes like alkaline phosphatase (ALP), alkaline aminotransferase (ALT), and aspartate aminotransferase (AST) were significantly reduced by Coccinia indica extract. CONCLUSION: Thus, the present results indicate the protective effect of Coccinia indica extract against CP-induced oxidative stress, genotoxicity, as well as hepatotoxicity. Medknow Publications & Media Pvt Ltd 2013 /pmc/articles/PMC3793523/ /pubmed/24130387 http://dx.doi.org/10.4103/0253-7613.117783 Text en Copyright: © Indian Journal of Pharmacology http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Nitharwal, Ramesh K Patel, Hasit Karchuli, Manvendra Singh Ugale, Rajesh Ramesh Chemoprotective potential of Coccinia indica against cyclophosphamide-induced toxicity |
title | Chemoprotective potential of Coccinia indica against cyclophosphamide-induced toxicity |
title_full | Chemoprotective potential of Coccinia indica against cyclophosphamide-induced toxicity |
title_fullStr | Chemoprotective potential of Coccinia indica against cyclophosphamide-induced toxicity |
title_full_unstemmed | Chemoprotective potential of Coccinia indica against cyclophosphamide-induced toxicity |
title_short | Chemoprotective potential of Coccinia indica against cyclophosphamide-induced toxicity |
title_sort | chemoprotective potential of coccinia indica against cyclophosphamide-induced toxicity |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3793523/ https://www.ncbi.nlm.nih.gov/pubmed/24130387 http://dx.doi.org/10.4103/0253-7613.117783 |
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