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Chemoprotective potential of Coccinia indica against cyclophosphamide-induced toxicity

OBJECTIVE: Although cyclophosphamide (CP), an alkylating agent, is used in the treatment of cancer owing to its broad-spectrum efficacy, its metabolites exhibit severe undesired toxicities in normal cells. The present study was aimed to investigate the chemoprotective potential of Coccinia indica ag...

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Autores principales: Nitharwal, Ramesh K, Patel, Hasit, Karchuli, Manvendra Singh, Ugale, Rajesh Ramesh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3793523/
https://www.ncbi.nlm.nih.gov/pubmed/24130387
http://dx.doi.org/10.4103/0253-7613.117783
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author Nitharwal, Ramesh K
Patel, Hasit
Karchuli, Manvendra Singh
Ugale, Rajesh Ramesh
author_facet Nitharwal, Ramesh K
Patel, Hasit
Karchuli, Manvendra Singh
Ugale, Rajesh Ramesh
author_sort Nitharwal, Ramesh K
collection PubMed
description OBJECTIVE: Although cyclophosphamide (CP), an alkylating agent, is used in the treatment of cancer owing to its broad-spectrum efficacy, its metabolites exhibit severe undesired toxicities in normal cells. The present study was aimed to investigate the chemoprotective potential of Coccinia indica against CP-induced oxidative stress, genotoxicity, and hepatotoxicity. MATERIALS AND METHODS: Rodents were orally pre-treated with Coccinia indica extract (200, 400, and 600 mg/kg) for five consecutive days. On 5th day, these animals were injected with CP (50 mg/kg i.p) and sacrificed after 24 hrs. for the evaluation of oxidative stress, hepatotoxicity, micronucleus formation, and chromosomal aberrations. RESULTS: We found that the CP significantly increased malondialdehyde (MDA) and decreased catalase and glutathione (GSH) levels in brain, and it was significantly reversed by Coccinia indica extract (400 and 600 mg/kg). Further, pre-treatment with Coccinia indica extract (200, 400, 600 mg/kg) significantly and dose-dependently reduced micronuclei formation and incidence of aberrant cells. We also found that the CP-induced increase in the serum biomarker enzymes like alkaline phosphatase (ALP), alkaline aminotransferase (ALT), and aspartate aminotransferase (AST) were significantly reduced by Coccinia indica extract. CONCLUSION: Thus, the present results indicate the protective effect of Coccinia indica extract against CP-induced oxidative stress, genotoxicity, as well as hepatotoxicity.
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spelling pubmed-37935232013-10-15 Chemoprotective potential of Coccinia indica against cyclophosphamide-induced toxicity Nitharwal, Ramesh K Patel, Hasit Karchuli, Manvendra Singh Ugale, Rajesh Ramesh Indian J Pharmacol Research Article OBJECTIVE: Although cyclophosphamide (CP), an alkylating agent, is used in the treatment of cancer owing to its broad-spectrum efficacy, its metabolites exhibit severe undesired toxicities in normal cells. The present study was aimed to investigate the chemoprotective potential of Coccinia indica against CP-induced oxidative stress, genotoxicity, and hepatotoxicity. MATERIALS AND METHODS: Rodents were orally pre-treated with Coccinia indica extract (200, 400, and 600 mg/kg) for five consecutive days. On 5th day, these animals were injected with CP (50 mg/kg i.p) and sacrificed after 24 hrs. for the evaluation of oxidative stress, hepatotoxicity, micronucleus formation, and chromosomal aberrations. RESULTS: We found that the CP significantly increased malondialdehyde (MDA) and decreased catalase and glutathione (GSH) levels in brain, and it was significantly reversed by Coccinia indica extract (400 and 600 mg/kg). Further, pre-treatment with Coccinia indica extract (200, 400, 600 mg/kg) significantly and dose-dependently reduced micronuclei formation and incidence of aberrant cells. We also found that the CP-induced increase in the serum biomarker enzymes like alkaline phosphatase (ALP), alkaline aminotransferase (ALT), and aspartate aminotransferase (AST) were significantly reduced by Coccinia indica extract. CONCLUSION: Thus, the present results indicate the protective effect of Coccinia indica extract against CP-induced oxidative stress, genotoxicity, as well as hepatotoxicity. Medknow Publications & Media Pvt Ltd 2013 /pmc/articles/PMC3793523/ /pubmed/24130387 http://dx.doi.org/10.4103/0253-7613.117783 Text en Copyright: © Indian Journal of Pharmacology http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Nitharwal, Ramesh K
Patel, Hasit
Karchuli, Manvendra Singh
Ugale, Rajesh Ramesh
Chemoprotective potential of Coccinia indica against cyclophosphamide-induced toxicity
title Chemoprotective potential of Coccinia indica against cyclophosphamide-induced toxicity
title_full Chemoprotective potential of Coccinia indica against cyclophosphamide-induced toxicity
title_fullStr Chemoprotective potential of Coccinia indica against cyclophosphamide-induced toxicity
title_full_unstemmed Chemoprotective potential of Coccinia indica against cyclophosphamide-induced toxicity
title_short Chemoprotective potential of Coccinia indica against cyclophosphamide-induced toxicity
title_sort chemoprotective potential of coccinia indica against cyclophosphamide-induced toxicity
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3793523/
https://www.ncbi.nlm.nih.gov/pubmed/24130387
http://dx.doi.org/10.4103/0253-7613.117783
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