Cargando…
CLIPing the brain: Studies of protein–RNA interactions important for neurodegenerative disorders()()
The fate of an mRNA is largely determined by its interactions with RNA binding proteins (RBPs). Post-transcriptional processing, RNA stability, localisation and translation are some of the events regulated by the plethora of RBPs present within cells. Mutations in various RBPs cause several diseases...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Academic Press
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3793874/ https://www.ncbi.nlm.nih.gov/pubmed/23583633 http://dx.doi.org/10.1016/j.mcn.2013.04.002 |
_version_ | 1782287135710117888 |
---|---|
author | Modic, Miha Ule, Jernej Sibley, Christopher R. |
author_facet | Modic, Miha Ule, Jernej Sibley, Christopher R. |
author_sort | Modic, Miha |
collection | PubMed |
description | The fate of an mRNA is largely determined by its interactions with RNA binding proteins (RBPs). Post-transcriptional processing, RNA stability, localisation and translation are some of the events regulated by the plethora of RBPs present within cells. Mutations in various RBPs cause several diseases of the central nervous system, including frontotemporal lobar degeneration, amyotrophic lateral sclerosis and fragile X syndrome. Here we review the studies that integrated UV-induced cross-linked immunoprecipitation (CLIP) with other genome-wide methods to comprehensively characterise the function of diverse RBPs in the brain. We discuss the technical challenges of these studies and review the strategies that can be used to reliably identify the RNAs bound and regulated by an RBP. We conclude by highlighting how CLIP and related techniques have been instrumental in addressing the role of RBPs in neurologic diseases. This article is part of a Special Issue entitled: RNA and splicing regulation in neurodegeneration. |
format | Online Article Text |
id | pubmed-3793874 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Academic Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-37938742013-10-10 CLIPing the brain: Studies of protein–RNA interactions important for neurodegenerative disorders()() Modic, Miha Ule, Jernej Sibley, Christopher R. Mol Cell Neurosci Article The fate of an mRNA is largely determined by its interactions with RNA binding proteins (RBPs). Post-transcriptional processing, RNA stability, localisation and translation are some of the events regulated by the plethora of RBPs present within cells. Mutations in various RBPs cause several diseases of the central nervous system, including frontotemporal lobar degeneration, amyotrophic lateral sclerosis and fragile X syndrome. Here we review the studies that integrated UV-induced cross-linked immunoprecipitation (CLIP) with other genome-wide methods to comprehensively characterise the function of diverse RBPs in the brain. We discuss the technical challenges of these studies and review the strategies that can be used to reliably identify the RNAs bound and regulated by an RBP. We conclude by highlighting how CLIP and related techniques have been instrumental in addressing the role of RBPs in neurologic diseases. This article is part of a Special Issue entitled: RNA and splicing regulation in neurodegeneration. Academic Press 2013-09 /pmc/articles/PMC3793874/ /pubmed/23583633 http://dx.doi.org/10.1016/j.mcn.2013.04.002 Text en © 2013 Elsevier Inc. https://creativecommons.org/licenses/by/3.0/ Open Access under CC BY 3.0 (https://creativecommons.org/licenses/by/3.0/) license |
spellingShingle | Article Modic, Miha Ule, Jernej Sibley, Christopher R. CLIPing the brain: Studies of protein–RNA interactions important for neurodegenerative disorders()() |
title | CLIPing the brain: Studies of protein–RNA interactions important for neurodegenerative disorders()() |
title_full | CLIPing the brain: Studies of protein–RNA interactions important for neurodegenerative disorders()() |
title_fullStr | CLIPing the brain: Studies of protein–RNA interactions important for neurodegenerative disorders()() |
title_full_unstemmed | CLIPing the brain: Studies of protein–RNA interactions important for neurodegenerative disorders()() |
title_short | CLIPing the brain: Studies of protein–RNA interactions important for neurodegenerative disorders()() |
title_sort | cliping the brain: studies of protein–rna interactions important for neurodegenerative disorders()() |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3793874/ https://www.ncbi.nlm.nih.gov/pubmed/23583633 http://dx.doi.org/10.1016/j.mcn.2013.04.002 |
work_keys_str_mv | AT modicmiha clipingthebrainstudiesofproteinrnainteractionsimportantforneurodegenerativedisorders AT ulejernej clipingthebrainstudiesofproteinrnainteractionsimportantforneurodegenerativedisorders AT sibleychristopherr clipingthebrainstudiesofproteinrnainteractionsimportantforneurodegenerativedisorders |