Cargando…

CLIPing the brain: Studies of protein–RNA interactions important for neurodegenerative disorders()()

The fate of an mRNA is largely determined by its interactions with RNA binding proteins (RBPs). Post-transcriptional processing, RNA stability, localisation and translation are some of the events regulated by the plethora of RBPs present within cells. Mutations in various RBPs cause several diseases...

Descripción completa

Detalles Bibliográficos
Autores principales: Modic, Miha, Ule, Jernej, Sibley, Christopher R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Academic Press 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3793874/
https://www.ncbi.nlm.nih.gov/pubmed/23583633
http://dx.doi.org/10.1016/j.mcn.2013.04.002
_version_ 1782287135710117888
author Modic, Miha
Ule, Jernej
Sibley, Christopher R.
author_facet Modic, Miha
Ule, Jernej
Sibley, Christopher R.
author_sort Modic, Miha
collection PubMed
description The fate of an mRNA is largely determined by its interactions with RNA binding proteins (RBPs). Post-transcriptional processing, RNA stability, localisation and translation are some of the events regulated by the plethora of RBPs present within cells. Mutations in various RBPs cause several diseases of the central nervous system, including frontotemporal lobar degeneration, amyotrophic lateral sclerosis and fragile X syndrome. Here we review the studies that integrated UV-induced cross-linked immunoprecipitation (CLIP) with other genome-wide methods to comprehensively characterise the function of diverse RBPs in the brain. We discuss the technical challenges of these studies and review the strategies that can be used to reliably identify the RNAs bound and regulated by an RBP. We conclude by highlighting how CLIP and related techniques have been instrumental in addressing the role of RBPs in neurologic diseases. This article is part of a Special Issue entitled: RNA and splicing regulation in neurodegeneration.
format Online
Article
Text
id pubmed-3793874
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Academic Press
record_format MEDLINE/PubMed
spelling pubmed-37938742013-10-10 CLIPing the brain: Studies of protein–RNA interactions important for neurodegenerative disorders()() Modic, Miha Ule, Jernej Sibley, Christopher R. Mol Cell Neurosci Article The fate of an mRNA is largely determined by its interactions with RNA binding proteins (RBPs). Post-transcriptional processing, RNA stability, localisation and translation are some of the events regulated by the plethora of RBPs present within cells. Mutations in various RBPs cause several diseases of the central nervous system, including frontotemporal lobar degeneration, amyotrophic lateral sclerosis and fragile X syndrome. Here we review the studies that integrated UV-induced cross-linked immunoprecipitation (CLIP) with other genome-wide methods to comprehensively characterise the function of diverse RBPs in the brain. We discuss the technical challenges of these studies and review the strategies that can be used to reliably identify the RNAs bound and regulated by an RBP. We conclude by highlighting how CLIP and related techniques have been instrumental in addressing the role of RBPs in neurologic diseases. This article is part of a Special Issue entitled: RNA and splicing regulation in neurodegeneration. Academic Press 2013-09 /pmc/articles/PMC3793874/ /pubmed/23583633 http://dx.doi.org/10.1016/j.mcn.2013.04.002 Text en © 2013 Elsevier Inc. https://creativecommons.org/licenses/by/3.0/ Open Access under CC BY 3.0 (https://creativecommons.org/licenses/by/3.0/) license
spellingShingle Article
Modic, Miha
Ule, Jernej
Sibley, Christopher R.
CLIPing the brain: Studies of protein–RNA interactions important for neurodegenerative disorders()()
title CLIPing the brain: Studies of protein–RNA interactions important for neurodegenerative disorders()()
title_full CLIPing the brain: Studies of protein–RNA interactions important for neurodegenerative disorders()()
title_fullStr CLIPing the brain: Studies of protein–RNA interactions important for neurodegenerative disorders()()
title_full_unstemmed CLIPing the brain: Studies of protein–RNA interactions important for neurodegenerative disorders()()
title_short CLIPing the brain: Studies of protein–RNA interactions important for neurodegenerative disorders()()
title_sort cliping the brain: studies of protein–rna interactions important for neurodegenerative disorders()()
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3793874/
https://www.ncbi.nlm.nih.gov/pubmed/23583633
http://dx.doi.org/10.1016/j.mcn.2013.04.002
work_keys_str_mv AT modicmiha clipingthebrainstudiesofproteinrnainteractionsimportantforneurodegenerativedisorders
AT ulejernej clipingthebrainstudiesofproteinrnainteractionsimportantforneurodegenerativedisorders
AT sibleychristopherr clipingthebrainstudiesofproteinrnainteractionsimportantforneurodegenerativedisorders