CRISPR RNA-guided activation of endogenous human genes

Catalytically inactive CRISPR-associated 9 nuclease (dCas9) can be directed by short guide RNAs (gRNAs) to repress endogenous genes in bacteria and human cells. Here we show that a dCas9-VP64 transcriptional activation domain fusion protein can be directed by single or multiple gRNAs to increase exp...

Descripción completa

Detalles Bibliográficos
Autores principales: Maeder, Morgan L, Linder, Samantha J, Cascio, Vincent M, Fu, Yanfang, Ho, Quan H, Joung, J Keith
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3794058/
https://www.ncbi.nlm.nih.gov/pubmed/23892898
http://dx.doi.org/10.1038/nmeth.2598
_version_ 1782287174900645888
author Maeder, Morgan L
Linder, Samantha J
Cascio, Vincent M
Fu, Yanfang
Ho, Quan H
Joung, J Keith
author_facet Maeder, Morgan L
Linder, Samantha J
Cascio, Vincent M
Fu, Yanfang
Ho, Quan H
Joung, J Keith
author_sort Maeder, Morgan L
collection PubMed
description Catalytically inactive CRISPR-associated 9 nuclease (dCas9) can be directed by short guide RNAs (gRNAs) to repress endogenous genes in bacteria and human cells. Here we show that a dCas9-VP64 transcriptional activation domain fusion protein can be directed by single or multiple gRNAs to increase expression of specific endogenous human genes. These results provide an important proof-of-principle that CRISPR-Cas systems can be used to target heterologous effector domains in human cells.
format Online
Article
Text
id pubmed-3794058
institution National Center for Biotechnology Information
language English
publishDate 2013
record_format MEDLINE/PubMed
spelling pubmed-37940582014-04-01 CRISPR RNA-guided activation of endogenous human genes Maeder, Morgan L Linder, Samantha J Cascio, Vincent M Fu, Yanfang Ho, Quan H Joung, J Keith Nat Methods Article Catalytically inactive CRISPR-associated 9 nuclease (dCas9) can be directed by short guide RNAs (gRNAs) to repress endogenous genes in bacteria and human cells. Here we show that a dCas9-VP64 transcriptional activation domain fusion protein can be directed by single or multiple gRNAs to increase expression of specific endogenous human genes. These results provide an important proof-of-principle that CRISPR-Cas systems can be used to target heterologous effector domains in human cells. 2013-07-25 2013-10 /pmc/articles/PMC3794058/ /pubmed/23892898 http://dx.doi.org/10.1038/nmeth.2598 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Maeder, Morgan L
Linder, Samantha J
Cascio, Vincent M
Fu, Yanfang
Ho, Quan H
Joung, J Keith
CRISPR RNA-guided activation of endogenous human genes
title CRISPR RNA-guided activation of endogenous human genes
title_full CRISPR RNA-guided activation of endogenous human genes
title_fullStr CRISPR RNA-guided activation of endogenous human genes
title_full_unstemmed CRISPR RNA-guided activation of endogenous human genes
title_short CRISPR RNA-guided activation of endogenous human genes
title_sort crispr rna-guided activation of endogenous human genes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3794058/
https://www.ncbi.nlm.nih.gov/pubmed/23892898
http://dx.doi.org/10.1038/nmeth.2598
work_keys_str_mv AT maedermorganl crisprrnaguidedactivationofendogenoushumangenes
AT lindersamanthaj crisprrnaguidedactivationofendogenoushumangenes
AT casciovincentm crisprrnaguidedactivationofendogenoushumangenes
AT fuyanfang crisprrnaguidedactivationofendogenoushumangenes
AT hoquanh crisprrnaguidedactivationofendogenoushumangenes
AT joungjkeith crisprrnaguidedactivationofendogenoushumangenes

Ejemplares similares