CRISPR RNA-guided activation of endogenous human genes

Catalytically inactive CRISPR-associated 9 nuclease (dCas9) can be directed by short guide RNAs (gRNAs) to repress endogenous genes in bacteria and human cells. Here we show that a dCas9-VP64 transcriptional activation domain fusion protein can be directed by single or multiple gRNAs to increase exp...

Descripción completa

Detalles Bibliográficos
Autores principales: Maeder, Morgan L, Linder, Samantha J, Cascio, Vincent M, Fu, Yanfang, Ho, Quan H, Joung, J Keith
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3794058/
https://www.ncbi.nlm.nih.gov/pubmed/23892898
http://dx.doi.org/10.1038/nmeth.2598

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3794058/
https://www.ncbi.nlm.nih.gov/pubmed/23892898
http://dx.doi.org/10.1038/nmeth.2598

Ejemplares similares