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Urethane anesthesia depresses activities of thalamocortical neurons and alters its response to nociception in terms of dual firing modes

Anesthetics are often used to characterize the activity of single neurons in vivo for their advantages such as reduction of noise level and convenience in noxious stimulations. Urethane has been a widely used anesthetic in thalamic studies under the assumption that sensory signals are still relayed...

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Autores principales: Huh, Yeowool, Cho, Jeiwon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3794191/
https://www.ncbi.nlm.nih.gov/pubmed/24133420
http://dx.doi.org/10.3389/fnbeh.2013.00141
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author Huh, Yeowool
Cho, Jeiwon
author_facet Huh, Yeowool
Cho, Jeiwon
author_sort Huh, Yeowool
collection PubMed
description Anesthetics are often used to characterize the activity of single neurons in vivo for their advantages such as reduction of noise level and convenience in noxious stimulations. Urethane has been a widely used anesthetic in thalamic studies under the assumption that sensory signals are still relayed to the thalamus under urethane anesthesia and that thalamic response would therefore reflect the response of the awake state. We tested this assumption by comparing thalamic activity in terms of tonic and burst firing modes during “the awake state” or under “urethane anesthesia” using the extracellular single unit recording technique. We first tested how thalamic relay neurons respond to the introduction of urethane, and then tested how urethane influences thalamic discharges under formalin-induced nociception. Urethane significantly depressed overall firing rates of thalamic relay neurons, which was sustained despite the delayed increase of burst activity over a 4 h recording period. Thalamic response to nociception under anesthesia was also similar overall except for the slight and transient increase of burst activity. Overall, results demonstrated that urethane suppresses the activity of thalamic relay neurons and that, despite the slight fluctuation of burst firing, formalin-induced nociception cannot significantly change the firing pattern of thalamic relay neurons that was caused by urethane.
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spelling pubmed-37941912013-10-16 Urethane anesthesia depresses activities of thalamocortical neurons and alters its response to nociception in terms of dual firing modes Huh, Yeowool Cho, Jeiwon Front Behav Neurosci Neuroscience Anesthetics are often used to characterize the activity of single neurons in vivo for their advantages such as reduction of noise level and convenience in noxious stimulations. Urethane has been a widely used anesthetic in thalamic studies under the assumption that sensory signals are still relayed to the thalamus under urethane anesthesia and that thalamic response would therefore reflect the response of the awake state. We tested this assumption by comparing thalamic activity in terms of tonic and burst firing modes during “the awake state” or under “urethane anesthesia” using the extracellular single unit recording technique. We first tested how thalamic relay neurons respond to the introduction of urethane, and then tested how urethane influences thalamic discharges under formalin-induced nociception. Urethane significantly depressed overall firing rates of thalamic relay neurons, which was sustained despite the delayed increase of burst activity over a 4 h recording period. Thalamic response to nociception under anesthesia was also similar overall except for the slight and transient increase of burst activity. Overall, results demonstrated that urethane suppresses the activity of thalamic relay neurons and that, despite the slight fluctuation of burst firing, formalin-induced nociception cannot significantly change the firing pattern of thalamic relay neurons that was caused by urethane. Frontiers Media S.A. 2013-10-10 /pmc/articles/PMC3794191/ /pubmed/24133420 http://dx.doi.org/10.3389/fnbeh.2013.00141 Text en Copyright © 2013 Huh and Cho. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Huh, Yeowool
Cho, Jeiwon
Urethane anesthesia depresses activities of thalamocortical neurons and alters its response to nociception in terms of dual firing modes
title Urethane anesthesia depresses activities of thalamocortical neurons and alters its response to nociception in terms of dual firing modes
title_full Urethane anesthesia depresses activities of thalamocortical neurons and alters its response to nociception in terms of dual firing modes
title_fullStr Urethane anesthesia depresses activities of thalamocortical neurons and alters its response to nociception in terms of dual firing modes
title_full_unstemmed Urethane anesthesia depresses activities of thalamocortical neurons and alters its response to nociception in terms of dual firing modes
title_short Urethane anesthesia depresses activities of thalamocortical neurons and alters its response to nociception in terms of dual firing modes
title_sort urethane anesthesia depresses activities of thalamocortical neurons and alters its response to nociception in terms of dual firing modes
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3794191/
https://www.ncbi.nlm.nih.gov/pubmed/24133420
http://dx.doi.org/10.3389/fnbeh.2013.00141
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