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In Vitro Reparative Dentin: a Biochemical and Morphological Study
In this study, starting from human dental pulp cells cultured in vitro, we simulated reparative dentinogenesis using a medium supplemented with different odontogenic inductors. The differentiation of dental pulp cells in odontoblast-like cells was evaluated by means of staining, and ultramorphologic...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PAGEPress Publications, Pavia, Italy
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3794354/ https://www.ncbi.nlm.nih.gov/pubmed/24085272 http://dx.doi.org/10.4081/ejh.2013.e23 |
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author | Teti, G. Salvatore, V. Ruggeri, A. Manzoli, L. Gesi, M. Orsini, G. Falconi, M. |
author_facet | Teti, G. Salvatore, V. Ruggeri, A. Manzoli, L. Gesi, M. Orsini, G. Falconi, M. |
author_sort | Teti, G. |
collection | PubMed |
description | In this study, starting from human dental pulp cells cultured in vitro, we simulated reparative dentinogenesis using a medium supplemented with different odontogenic inductors. The differentiation of dental pulp cells in odontoblast-like cells was evaluated by means of staining, and ultramorphological, biochemical and biomolecular methods. Alizarin red staining showed mineral deposition while transmission electron microscopy revealed a synthesis of extracellular matrix fibers during the differentiation process. Biochemical assays demonstrated that the differentiated phenotype expressed odontoblast markers, such as Dentin Matrix Protein 1 (DMP1) and Dentin Sialoprotein (DSP), as well as type I collagen. Quantitative data regarding the mRNA expression of DMP1, DSP and type I collagen were obtained by Real Time PCR. Immunofluorescence data demonstrated the various localizations of DSP and DMP1 during odontoblast differentiation. Based on our results, we obtained odontoblast-like cells which simulated the reparative dentin processes in order to better investigate the mechanism of odontoblast differentiation, and dentin extracellular matrix deposition and mineralization. |
format | Online Article Text |
id | pubmed-3794354 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | PAGEPress Publications, Pavia, Italy |
record_format | MEDLINE/PubMed |
spelling | pubmed-37943542013-10-21 In Vitro Reparative Dentin: a Biochemical and Morphological Study Teti, G. Salvatore, V. Ruggeri, A. Manzoli, L. Gesi, M. Orsini, G. Falconi, M. Eur J Histochem Original Paper In this study, starting from human dental pulp cells cultured in vitro, we simulated reparative dentinogenesis using a medium supplemented with different odontogenic inductors. The differentiation of dental pulp cells in odontoblast-like cells was evaluated by means of staining, and ultramorphological, biochemical and biomolecular methods. Alizarin red staining showed mineral deposition while transmission electron microscopy revealed a synthesis of extracellular matrix fibers during the differentiation process. Biochemical assays demonstrated that the differentiated phenotype expressed odontoblast markers, such as Dentin Matrix Protein 1 (DMP1) and Dentin Sialoprotein (DSP), as well as type I collagen. Quantitative data regarding the mRNA expression of DMP1, DSP and type I collagen were obtained by Real Time PCR. Immunofluorescence data demonstrated the various localizations of DSP and DMP1 during odontoblast differentiation. Based on our results, we obtained odontoblast-like cells which simulated the reparative dentin processes in order to better investigate the mechanism of odontoblast differentiation, and dentin extracellular matrix deposition and mineralization. PAGEPress Publications, Pavia, Italy 2013-08-06 /pmc/articles/PMC3794354/ /pubmed/24085272 http://dx.doi.org/10.4081/ejh.2013.e23 Text en ©Copyright G. Teti et al. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Paper Teti, G. Salvatore, V. Ruggeri, A. Manzoli, L. Gesi, M. Orsini, G. Falconi, M. In Vitro Reparative Dentin: a Biochemical and Morphological Study |
title | In Vitro Reparative Dentin: a Biochemical and Morphological Study |
title_full | In Vitro Reparative Dentin: a Biochemical and Morphological Study |
title_fullStr | In Vitro Reparative Dentin: a Biochemical and Morphological Study |
title_full_unstemmed | In Vitro Reparative Dentin: a Biochemical and Morphological Study |
title_short | In Vitro Reparative Dentin: a Biochemical and Morphological Study |
title_sort | in vitro reparative dentin: a biochemical and morphological study |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3794354/ https://www.ncbi.nlm.nih.gov/pubmed/24085272 http://dx.doi.org/10.4081/ejh.2013.e23 |
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