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Gene Expression Profiling on the Molecular Action of Danshen-Gegen Formula in a Randomized Placebo-Controlled Trial of Postmenopausal Women with Hypercholesterolemia

The Danshen-Gegen formula (DG) is a traditional Chinese herbal formula which has long been used to treat cardiovascular disease. DG was found to be a cardiovascular tonic in our recent research. However, a comprehensive investigation of the molecular mechanism of DG in cardiovascular disease has not...

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Autores principales: Koon, Chi-Man, Ko, Chun-Hay, Sun, Xu-Xu, Hoi, Sandy Wan-Heng, Tam, Jacqueline Chor-Wing, Cheung, David Wing-Shing, Cheng, King-Fai, Pang, Suet-Yee, Lo, Wing-Man, Chook, Ping, Lau, Clara Bik-San, Chan, Wai-Yee, Leung, Ping-Chung, Kwok, Timothy Chi-Yui, Fung, Kwok-Pui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3794622/
https://www.ncbi.nlm.nih.gov/pubmed/24174980
http://dx.doi.org/10.1155/2013/703705
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author Koon, Chi-Man
Ko, Chun-Hay
Sun, Xu-Xu
Hoi, Sandy Wan-Heng
Tam, Jacqueline Chor-Wing
Cheung, David Wing-Shing
Cheng, King-Fai
Pang, Suet-Yee
Lo, Wing-Man
Chook, Ping
Lau, Clara Bik-San
Chan, Wai-Yee
Leung, Ping-Chung
Kwok, Timothy Chi-Yui
Fung, Kwok-Pui
author_facet Koon, Chi-Man
Ko, Chun-Hay
Sun, Xu-Xu
Hoi, Sandy Wan-Heng
Tam, Jacqueline Chor-Wing
Cheung, David Wing-Shing
Cheng, King-Fai
Pang, Suet-Yee
Lo, Wing-Man
Chook, Ping
Lau, Clara Bik-San
Chan, Wai-Yee
Leung, Ping-Chung
Kwok, Timothy Chi-Yui
Fung, Kwok-Pui
author_sort Koon, Chi-Man
collection PubMed
description The Danshen-Gegen formula (DG) is a traditional Chinese herbal formula which has long been used to treat cardiovascular disease. DG was found to be a cardiovascular tonic in our recent research. However, a comprehensive investigation of the molecular mechanism of DG in cardiovascular disease has not been performed. The aim of this study was to clarify the transcriptional profiling of genes modulated by DG on postmenopausal women by using DNAmicroarray technology. We obtained 29 whole blood samples both from DG-treated and placebo-treated subjects. Blood lipid profile and intima-media thickness (IMT) were measured. Affymetrix GeneChip was used to identify differentially expressed genes (DEGs), followed by validation by the real-time PCR method. The results showed that DG-treated group has a significant improvement in IMT and lipid profile as compared to placebo-treated group. For the genomic study, the DG-treated group has a higher number of DEGs identified as compared to the placebo-treated group. Two important biological processes of “regulation of systemic arterial blood pressure by hormone” and “regulation of smooth muscle proliferation” have been identified by GePS in the DG-treated group. No significant biological process and cellular components were identified in the placebo-treated group. This genomic study on the molecular action of DG in postmenopausal women gathered sufficient molecular targets and pathways to reveal that DG could improve neointima thickening and hypertension.
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spelling pubmed-37946222013-10-30 Gene Expression Profiling on the Molecular Action of Danshen-Gegen Formula in a Randomized Placebo-Controlled Trial of Postmenopausal Women with Hypercholesterolemia Koon, Chi-Man Ko, Chun-Hay Sun, Xu-Xu Hoi, Sandy Wan-Heng Tam, Jacqueline Chor-Wing Cheung, David Wing-Shing Cheng, King-Fai Pang, Suet-Yee Lo, Wing-Man Chook, Ping Lau, Clara Bik-San Chan, Wai-Yee Leung, Ping-Chung Kwok, Timothy Chi-Yui Fung, Kwok-Pui Evid Based Complement Alternat Med Research Article The Danshen-Gegen formula (DG) is a traditional Chinese herbal formula which has long been used to treat cardiovascular disease. DG was found to be a cardiovascular tonic in our recent research. However, a comprehensive investigation of the molecular mechanism of DG in cardiovascular disease has not been performed. The aim of this study was to clarify the transcriptional profiling of genes modulated by DG on postmenopausal women by using DNAmicroarray technology. We obtained 29 whole blood samples both from DG-treated and placebo-treated subjects. Blood lipid profile and intima-media thickness (IMT) were measured. Affymetrix GeneChip was used to identify differentially expressed genes (DEGs), followed by validation by the real-time PCR method. The results showed that DG-treated group has a significant improvement in IMT and lipid profile as compared to placebo-treated group. For the genomic study, the DG-treated group has a higher number of DEGs identified as compared to the placebo-treated group. Two important biological processes of “regulation of systemic arterial blood pressure by hormone” and “regulation of smooth muscle proliferation” have been identified by GePS in the DG-treated group. No significant biological process and cellular components were identified in the placebo-treated group. This genomic study on the molecular action of DG in postmenopausal women gathered sufficient molecular targets and pathways to reveal that DG could improve neointima thickening and hypertension. Hindawi Publishing Corporation 2013 2013-09-23 /pmc/articles/PMC3794622/ /pubmed/24174980 http://dx.doi.org/10.1155/2013/703705 Text en Copyright © 2013 Chi-Man Koon et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Koon, Chi-Man
Ko, Chun-Hay
Sun, Xu-Xu
Hoi, Sandy Wan-Heng
Tam, Jacqueline Chor-Wing
Cheung, David Wing-Shing
Cheng, King-Fai
Pang, Suet-Yee
Lo, Wing-Man
Chook, Ping
Lau, Clara Bik-San
Chan, Wai-Yee
Leung, Ping-Chung
Kwok, Timothy Chi-Yui
Fung, Kwok-Pui
Gene Expression Profiling on the Molecular Action of Danshen-Gegen Formula in a Randomized Placebo-Controlled Trial of Postmenopausal Women with Hypercholesterolemia
title Gene Expression Profiling on the Molecular Action of Danshen-Gegen Formula in a Randomized Placebo-Controlled Trial of Postmenopausal Women with Hypercholesterolemia
title_full Gene Expression Profiling on the Molecular Action of Danshen-Gegen Formula in a Randomized Placebo-Controlled Trial of Postmenopausal Women with Hypercholesterolemia
title_fullStr Gene Expression Profiling on the Molecular Action of Danshen-Gegen Formula in a Randomized Placebo-Controlled Trial of Postmenopausal Women with Hypercholesterolemia
title_full_unstemmed Gene Expression Profiling on the Molecular Action of Danshen-Gegen Formula in a Randomized Placebo-Controlled Trial of Postmenopausal Women with Hypercholesterolemia
title_short Gene Expression Profiling on the Molecular Action of Danshen-Gegen Formula in a Randomized Placebo-Controlled Trial of Postmenopausal Women with Hypercholesterolemia
title_sort gene expression profiling on the molecular action of danshen-gegen formula in a randomized placebo-controlled trial of postmenopausal women with hypercholesterolemia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3794622/
https://www.ncbi.nlm.nih.gov/pubmed/24174980
http://dx.doi.org/10.1155/2013/703705
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