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Skeletal Muscle Regeneration on Protein-Grafted and Microchannel-Patterned Scaffold for Hypopharyngeal Tissue Engineering

In the field of tissue engineering, polymeric materials with high biocompatibility like polylactic acid and polyglycolic acid have been widely used for fabricating living constructs. For hypopharynx tissue engineering, skeletal muscle is one important functional part of the whole organ, which assemb...

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Autores principales: Shen, Zhisen, Guo, Shanshan, Ye, Dong, Chen, Jingjing, Kang, Cheng, Qiu, Shejie, Lu, Dakai, Li, Qun, Xu, Kunjie, Lv, Jingjing, Zhu, Yabin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3794632/
https://www.ncbi.nlm.nih.gov/pubmed/24175281
http://dx.doi.org/10.1155/2013/146953
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author Shen, Zhisen
Guo, Shanshan
Ye, Dong
Chen, Jingjing
Kang, Cheng
Qiu, Shejie
Lu, Dakai
Li, Qun
Xu, Kunjie
Lv, Jingjing
Zhu, Yabin
author_facet Shen, Zhisen
Guo, Shanshan
Ye, Dong
Chen, Jingjing
Kang, Cheng
Qiu, Shejie
Lu, Dakai
Li, Qun
Xu, Kunjie
Lv, Jingjing
Zhu, Yabin
author_sort Shen, Zhisen
collection PubMed
description In the field of tissue engineering, polymeric materials with high biocompatibility like polylactic acid and polyglycolic acid have been widely used for fabricating living constructs. For hypopharynx tissue engineering, skeletal muscle is one important functional part of the whole organ, which assembles the unidirectionally aligned myotubes. In this study, a polyurethane (PU) scaffold with microchannel patterns was used to provide aligning guidance for the seeded human myoblasts. Due to the low hydrophilicity of PU, the scaffold was grafted with silk fibroin (PU-SF) or gelatin (PU-Gel) to improve its cell adhesion properties. Scaffolds were observed to degrade slowly over time, and their mechanical properties and hydrophilicities were improved through the surface grafting. Also, the myoblasts seeded on PU-SF had the higher proliferative rate and better differentiation compared with those on the control or PU-Gel. Our results demonstrate that polyurethane scaffolds seeded with myoblasts hold promise to guide hypopharynx muscle regeneration.
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spelling pubmed-37946322013-10-30 Skeletal Muscle Regeneration on Protein-Grafted and Microchannel-Patterned Scaffold for Hypopharyngeal Tissue Engineering Shen, Zhisen Guo, Shanshan Ye, Dong Chen, Jingjing Kang, Cheng Qiu, Shejie Lu, Dakai Li, Qun Xu, Kunjie Lv, Jingjing Zhu, Yabin Biomed Res Int Research Article In the field of tissue engineering, polymeric materials with high biocompatibility like polylactic acid and polyglycolic acid have been widely used for fabricating living constructs. For hypopharynx tissue engineering, skeletal muscle is one important functional part of the whole organ, which assembles the unidirectionally aligned myotubes. In this study, a polyurethane (PU) scaffold with microchannel patterns was used to provide aligning guidance for the seeded human myoblasts. Due to the low hydrophilicity of PU, the scaffold was grafted with silk fibroin (PU-SF) or gelatin (PU-Gel) to improve its cell adhesion properties. Scaffolds were observed to degrade slowly over time, and their mechanical properties and hydrophilicities were improved through the surface grafting. Also, the myoblasts seeded on PU-SF had the higher proliferative rate and better differentiation compared with those on the control or PU-Gel. Our results demonstrate that polyurethane scaffolds seeded with myoblasts hold promise to guide hypopharynx muscle regeneration. Hindawi Publishing Corporation 2013 2013-09-23 /pmc/articles/PMC3794632/ /pubmed/24175281 http://dx.doi.org/10.1155/2013/146953 Text en Copyright © 2013 Zhisen Shen et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Shen, Zhisen
Guo, Shanshan
Ye, Dong
Chen, Jingjing
Kang, Cheng
Qiu, Shejie
Lu, Dakai
Li, Qun
Xu, Kunjie
Lv, Jingjing
Zhu, Yabin
Skeletal Muscle Regeneration on Protein-Grafted and Microchannel-Patterned Scaffold for Hypopharyngeal Tissue Engineering
title Skeletal Muscle Regeneration on Protein-Grafted and Microchannel-Patterned Scaffold for Hypopharyngeal Tissue Engineering
title_full Skeletal Muscle Regeneration on Protein-Grafted and Microchannel-Patterned Scaffold for Hypopharyngeal Tissue Engineering
title_fullStr Skeletal Muscle Regeneration on Protein-Grafted and Microchannel-Patterned Scaffold for Hypopharyngeal Tissue Engineering
title_full_unstemmed Skeletal Muscle Regeneration on Protein-Grafted and Microchannel-Patterned Scaffold for Hypopharyngeal Tissue Engineering
title_short Skeletal Muscle Regeneration on Protein-Grafted and Microchannel-Patterned Scaffold for Hypopharyngeal Tissue Engineering
title_sort skeletal muscle regeneration on protein-grafted and microchannel-patterned scaffold for hypopharyngeal tissue engineering
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3794632/
https://www.ncbi.nlm.nih.gov/pubmed/24175281
http://dx.doi.org/10.1155/2013/146953
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