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Molecular Identification of Ancylostoma caninum Isolated from Cats in Southern China Based on Complete ITS Sequence
Ancylostoma caninum is a blood-feeding parasitic intestinal nematode which infects dogs, cats, and other mammals throughout the world. A highly sensitive and species-specific PCR-RFLP technique was utilised to detect the prevalence of A. caninum in cats in Guangzhou, southern China. Of the 102 fecal...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Hindawi Publishing Corporation
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3794661/ https://www.ncbi.nlm.nih.gov/pubmed/24175305 http://dx.doi.org/10.1155/2013/868050 |
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author | Liu, Yuanjia Zheng, Guochao Alsarakibi, Muhamd Zhang, Xinheng Hu, Wei Lu, Pengyun Lin, Liqin Tan, Liping Luo, Qin Li, Guoqing |
author_facet | Liu, Yuanjia Zheng, Guochao Alsarakibi, Muhamd Zhang, Xinheng Hu, Wei Lu, Pengyun Lin, Liqin Tan, Liping Luo, Qin Li, Guoqing |
author_sort | Liu, Yuanjia |
collection | PubMed |
description | Ancylostoma caninum is a blood-feeding parasitic intestinal nematode which infects dogs, cats, and other mammals throughout the world. A highly sensitive and species-specific PCR-RFLP technique was utilised to detect the prevalence of A. caninum in cats in Guangzhou, southern China. Of the 102 fecal samples examined, the prevalence of A. caninum in cats was 95.1% and 83.3% using PCR-RFLP and microscopy, respectively. Among them, the prevalence of single hookworm infection with A. caninum was 54.90%, while mixed infections with both A. caninum and A. ceylanicum were 40.20%. Comparative analysis of three complete ITS sequences obtained from cat-derived A. caninum showed the same length (738 bp) as that of dog-derived A. caninum. However, the sequence variation range was 98.6%–100%, where only one cat isolate (M63) showed 100% sequence similarity in comparison with two dog-derived A. caninum isolates (AM850106, EU159416) in the same studied area. The phylogenetic tree revealed A. caninum derived from both cats and dogs in single cluster. Results suggest that cats could be the main host of A. caninum in China, which may cause cross-infection between dogs and cats in the same area. |
format | Online Article Text |
id | pubmed-3794661 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-37946612013-10-30 Molecular Identification of Ancylostoma caninum Isolated from Cats in Southern China Based on Complete ITS Sequence Liu, Yuanjia Zheng, Guochao Alsarakibi, Muhamd Zhang, Xinheng Hu, Wei Lu, Pengyun Lin, Liqin Tan, Liping Luo, Qin Li, Guoqing Biomed Res Int Research Article Ancylostoma caninum is a blood-feeding parasitic intestinal nematode which infects dogs, cats, and other mammals throughout the world. A highly sensitive and species-specific PCR-RFLP technique was utilised to detect the prevalence of A. caninum in cats in Guangzhou, southern China. Of the 102 fecal samples examined, the prevalence of A. caninum in cats was 95.1% and 83.3% using PCR-RFLP and microscopy, respectively. Among them, the prevalence of single hookworm infection with A. caninum was 54.90%, while mixed infections with both A. caninum and A. ceylanicum were 40.20%. Comparative analysis of three complete ITS sequences obtained from cat-derived A. caninum showed the same length (738 bp) as that of dog-derived A. caninum. However, the sequence variation range was 98.6%–100%, where only one cat isolate (M63) showed 100% sequence similarity in comparison with two dog-derived A. caninum isolates (AM850106, EU159416) in the same studied area. The phylogenetic tree revealed A. caninum derived from both cats and dogs in single cluster. Results suggest that cats could be the main host of A. caninum in China, which may cause cross-infection between dogs and cats in the same area. Hindawi Publishing Corporation 2013 2013-09-23 /pmc/articles/PMC3794661/ /pubmed/24175305 http://dx.doi.org/10.1155/2013/868050 Text en Copyright © 2013 Yuanjia Liu et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Liu, Yuanjia Zheng, Guochao Alsarakibi, Muhamd Zhang, Xinheng Hu, Wei Lu, Pengyun Lin, Liqin Tan, Liping Luo, Qin Li, Guoqing Molecular Identification of Ancylostoma caninum Isolated from Cats in Southern China Based on Complete ITS Sequence |
title | Molecular Identification of Ancylostoma caninum Isolated from Cats in Southern China Based on Complete ITS Sequence |
title_full | Molecular Identification of Ancylostoma caninum Isolated from Cats in Southern China Based on Complete ITS Sequence |
title_fullStr | Molecular Identification of Ancylostoma caninum Isolated from Cats in Southern China Based on Complete ITS Sequence |
title_full_unstemmed | Molecular Identification of Ancylostoma caninum Isolated from Cats in Southern China Based on Complete ITS Sequence |
title_short | Molecular Identification of Ancylostoma caninum Isolated from Cats in Southern China Based on Complete ITS Sequence |
title_sort | molecular identification of ancylostoma caninum isolated from cats in southern china based on complete its sequence |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3794661/ https://www.ncbi.nlm.nih.gov/pubmed/24175305 http://dx.doi.org/10.1155/2013/868050 |
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