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Protective Effect of N-Acetylserotonin against Acute Hepatic Ischemia-Reperfusion Injury in Mice

The purpose of this study was to investigate the possible protective effect of N-acetylserotonin (NAS) against acute hepatic ischemia-reperfusion (I/R) injury in mice. Adult male mice were randomly divided into three groups: sham, I/R, and I/R + NAS. The hepatic I/R injury model was generated by cla...

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Autores principales: Yu, Shuna, Zheng, Jie, Jiang, Zhengchen, Shi, Caixing, Li, Jin, Du, Xiaodong, Wang, Hailiang, Jiang, Jiying, Wang, Xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3794748/
https://www.ncbi.nlm.nih.gov/pubmed/23994834
http://dx.doi.org/10.3390/ijms140917680
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author Yu, Shuna
Zheng, Jie
Jiang, Zhengchen
Shi, Caixing
Li, Jin
Du, Xiaodong
Wang, Hailiang
Jiang, Jiying
Wang, Xin
author_facet Yu, Shuna
Zheng, Jie
Jiang, Zhengchen
Shi, Caixing
Li, Jin
Du, Xiaodong
Wang, Hailiang
Jiang, Jiying
Wang, Xin
author_sort Yu, Shuna
collection PubMed
description The purpose of this study was to investigate the possible protective effect of N-acetylserotonin (NAS) against acute hepatic ischemia-reperfusion (I/R) injury in mice. Adult male mice were randomly divided into three groups: sham, I/R, and I/R + NAS. The hepatic I/R injury model was generated by clamping the hepatic artery, portal vein, and common bile duct with a microvascular bulldog clamp for 30 min, and then removing the clamp and allowing reperfusion for 6 h. Morphologic changes and hepatocyte apoptosis were evaluated by hematoxylin-eosin (HE) and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining, respectively. Activated caspase-3 expression was evaluated by immunohistochemistry and Western blot. The activation of aspartate aminotransferase (AST), malondialdehyde (MDA), and superoxide dismutase (SOD) was evaluated by enzyme-linked immunosorbent assay (ELISA). The data show that NAS rescued hepatocyte morphological damage and dysfunction, decreased the number of apoptotic hepatocytes, and reduced caspase-3 activation. Our work demonstrates that NAS ameliorates hepatic IR injury.
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spelling pubmed-37947482013-10-21 Protective Effect of N-Acetylserotonin against Acute Hepatic Ischemia-Reperfusion Injury in Mice Yu, Shuna Zheng, Jie Jiang, Zhengchen Shi, Caixing Li, Jin Du, Xiaodong Wang, Hailiang Jiang, Jiying Wang, Xin Int J Mol Sci Article The purpose of this study was to investigate the possible protective effect of N-acetylserotonin (NAS) against acute hepatic ischemia-reperfusion (I/R) injury in mice. Adult male mice were randomly divided into three groups: sham, I/R, and I/R + NAS. The hepatic I/R injury model was generated by clamping the hepatic artery, portal vein, and common bile duct with a microvascular bulldog clamp for 30 min, and then removing the clamp and allowing reperfusion for 6 h. Morphologic changes and hepatocyte apoptosis were evaluated by hematoxylin-eosin (HE) and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining, respectively. Activated caspase-3 expression was evaluated by immunohistochemistry and Western blot. The activation of aspartate aminotransferase (AST), malondialdehyde (MDA), and superoxide dismutase (SOD) was evaluated by enzyme-linked immunosorbent assay (ELISA). The data show that NAS rescued hepatocyte morphological damage and dysfunction, decreased the number of apoptotic hepatocytes, and reduced caspase-3 activation. Our work demonstrates that NAS ameliorates hepatic IR injury. MDPI 2013-08-29 /pmc/articles/PMC3794748/ /pubmed/23994834 http://dx.doi.org/10.3390/ijms140917680 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0 This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Yu, Shuna
Zheng, Jie
Jiang, Zhengchen
Shi, Caixing
Li, Jin
Du, Xiaodong
Wang, Hailiang
Jiang, Jiying
Wang, Xin
Protective Effect of N-Acetylserotonin against Acute Hepatic Ischemia-Reperfusion Injury in Mice
title Protective Effect of N-Acetylserotonin against Acute Hepatic Ischemia-Reperfusion Injury in Mice
title_full Protective Effect of N-Acetylserotonin against Acute Hepatic Ischemia-Reperfusion Injury in Mice
title_fullStr Protective Effect of N-Acetylserotonin against Acute Hepatic Ischemia-Reperfusion Injury in Mice
title_full_unstemmed Protective Effect of N-Acetylserotonin against Acute Hepatic Ischemia-Reperfusion Injury in Mice
title_short Protective Effect of N-Acetylserotonin against Acute Hepatic Ischemia-Reperfusion Injury in Mice
title_sort protective effect of n-acetylserotonin against acute hepatic ischemia-reperfusion injury in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3794748/
https://www.ncbi.nlm.nih.gov/pubmed/23994834
http://dx.doi.org/10.3390/ijms140917680
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