Cargando…

Ziyuglycoside II Inhibits the Growth of Human Breast Carcinoma MDA-MB-435 Cells via Cell Cycle Arrest and Induction of Apoptosis through the Mitochondria Dependent Pathway

Ziyuglycoside II is one of the major active compounds of Sanguisorba officinalis L., which has a wide range of clinical applications including hemostasis, antibiosis, anti-inflammation and anti-oxidation. This study investigated the effect of ziyuglycoside II on the growth of human breast carcinoma...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhu, Xue, Wang, Ke, Zhang, Kai, Huang, Biao, Zhang, Jue, Zhang, Yi, Zhu, Lan, Zhou, Bin, Zhou, Fanfan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3794768/
https://www.ncbi.nlm.nih.gov/pubmed/24005866
http://dx.doi.org/10.3390/ijms140918041
_version_ 1782287252577058816
author Zhu, Xue
Wang, Ke
Zhang, Kai
Huang, Biao
Zhang, Jue
Zhang, Yi
Zhu, Lan
Zhou, Bin
Zhou, Fanfan
author_facet Zhu, Xue
Wang, Ke
Zhang, Kai
Huang, Biao
Zhang, Jue
Zhang, Yi
Zhu, Lan
Zhou, Bin
Zhou, Fanfan
author_sort Zhu, Xue
collection PubMed
description Ziyuglycoside II is one of the major active compounds of Sanguisorba officinalis L., which has a wide range of clinical applications including hemostasis, antibiosis, anti-inflammation and anti-oxidation. This study investigated the effect of ziyuglycoside II on the growth of human breast carcinoma MDA-MB-435 cells for the first time. The results showed that ziyuglycoside II could significantly inhibit the growth of MDA-MB-435 cells through blocking cell cycle progression at G0/G1 and S phase as well as via inducing cell apoptosis. Accumulation of reactive oxygen species (ROS) was observed in the progression of cell cycle arrest, which was associated with the increased expression of cell cycle regulating factors, p53 and p21. Subsequent apoptosis induced by ziyuglycoside II was accompanied with the activation of mitochondrial pathway, in particular a decreased mitochondrial membrane potential (MMP) as well as increased Bax/Bcl-2 ratio, cytochrome c release and the activity of caspase-3 and caspase-9. In conclusion, our study was the first to report that ziyuglycoside II has inhibitory effect on the growth of MDA-MB-435 cells, which might become a potential therapeutic approach of breast cancer in the future.
format Online
Article
Text
id pubmed-3794768
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-37947682013-10-21 Ziyuglycoside II Inhibits the Growth of Human Breast Carcinoma MDA-MB-435 Cells via Cell Cycle Arrest and Induction of Apoptosis through the Mitochondria Dependent Pathway Zhu, Xue Wang, Ke Zhang, Kai Huang, Biao Zhang, Jue Zhang, Yi Zhu, Lan Zhou, Bin Zhou, Fanfan Int J Mol Sci Article Ziyuglycoside II is one of the major active compounds of Sanguisorba officinalis L., which has a wide range of clinical applications including hemostasis, antibiosis, anti-inflammation and anti-oxidation. This study investigated the effect of ziyuglycoside II on the growth of human breast carcinoma MDA-MB-435 cells for the first time. The results showed that ziyuglycoside II could significantly inhibit the growth of MDA-MB-435 cells through blocking cell cycle progression at G0/G1 and S phase as well as via inducing cell apoptosis. Accumulation of reactive oxygen species (ROS) was observed in the progression of cell cycle arrest, which was associated with the increased expression of cell cycle regulating factors, p53 and p21. Subsequent apoptosis induced by ziyuglycoside II was accompanied with the activation of mitochondrial pathway, in particular a decreased mitochondrial membrane potential (MMP) as well as increased Bax/Bcl-2 ratio, cytochrome c release and the activity of caspase-3 and caspase-9. In conclusion, our study was the first to report that ziyuglycoside II has inhibitory effect on the growth of MDA-MB-435 cells, which might become a potential therapeutic approach of breast cancer in the future. MDPI 2013-09-03 /pmc/articles/PMC3794768/ /pubmed/24005866 http://dx.doi.org/10.3390/ijms140918041 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0 This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Zhu, Xue
Wang, Ke
Zhang, Kai
Huang, Biao
Zhang, Jue
Zhang, Yi
Zhu, Lan
Zhou, Bin
Zhou, Fanfan
Ziyuglycoside II Inhibits the Growth of Human Breast Carcinoma MDA-MB-435 Cells via Cell Cycle Arrest and Induction of Apoptosis through the Mitochondria Dependent Pathway
title Ziyuglycoside II Inhibits the Growth of Human Breast Carcinoma MDA-MB-435 Cells via Cell Cycle Arrest and Induction of Apoptosis through the Mitochondria Dependent Pathway
title_full Ziyuglycoside II Inhibits the Growth of Human Breast Carcinoma MDA-MB-435 Cells via Cell Cycle Arrest and Induction of Apoptosis through the Mitochondria Dependent Pathway
title_fullStr Ziyuglycoside II Inhibits the Growth of Human Breast Carcinoma MDA-MB-435 Cells via Cell Cycle Arrest and Induction of Apoptosis through the Mitochondria Dependent Pathway
title_full_unstemmed Ziyuglycoside II Inhibits the Growth of Human Breast Carcinoma MDA-MB-435 Cells via Cell Cycle Arrest and Induction of Apoptosis through the Mitochondria Dependent Pathway
title_short Ziyuglycoside II Inhibits the Growth of Human Breast Carcinoma MDA-MB-435 Cells via Cell Cycle Arrest and Induction of Apoptosis through the Mitochondria Dependent Pathway
title_sort ziyuglycoside ii inhibits the growth of human breast carcinoma mda-mb-435 cells via cell cycle arrest and induction of apoptosis through the mitochondria dependent pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3794768/
https://www.ncbi.nlm.nih.gov/pubmed/24005866
http://dx.doi.org/10.3390/ijms140918041
work_keys_str_mv AT zhuxue ziyuglycosideiiinhibitsthegrowthofhumanbreastcarcinomamdamb435cellsviacellcyclearrestandinductionofapoptosisthroughthemitochondriadependentpathway
AT wangke ziyuglycosideiiinhibitsthegrowthofhumanbreastcarcinomamdamb435cellsviacellcyclearrestandinductionofapoptosisthroughthemitochondriadependentpathway
AT zhangkai ziyuglycosideiiinhibitsthegrowthofhumanbreastcarcinomamdamb435cellsviacellcyclearrestandinductionofapoptosisthroughthemitochondriadependentpathway
AT huangbiao ziyuglycosideiiinhibitsthegrowthofhumanbreastcarcinomamdamb435cellsviacellcyclearrestandinductionofapoptosisthroughthemitochondriadependentpathway
AT zhangjue ziyuglycosideiiinhibitsthegrowthofhumanbreastcarcinomamdamb435cellsviacellcyclearrestandinductionofapoptosisthroughthemitochondriadependentpathway
AT zhangyi ziyuglycosideiiinhibitsthegrowthofhumanbreastcarcinomamdamb435cellsviacellcyclearrestandinductionofapoptosisthroughthemitochondriadependentpathway
AT zhulan ziyuglycosideiiinhibitsthegrowthofhumanbreastcarcinomamdamb435cellsviacellcyclearrestandinductionofapoptosisthroughthemitochondriadependentpathway
AT zhoubin ziyuglycosideiiinhibitsthegrowthofhumanbreastcarcinomamdamb435cellsviacellcyclearrestandinductionofapoptosisthroughthemitochondriadependentpathway
AT zhoufanfan ziyuglycosideiiinhibitsthegrowthofhumanbreastcarcinomamdamb435cellsviacellcyclearrestandinductionofapoptosisthroughthemitochondriadependentpathway