Cargando…

Voltage-Gated Potassium Channel Kv1.3 Is Highly Expressed in Human Osteosarcoma and Promotes Osteosarcoma Growth

Deregulation of voltage-gated potassium channel subunit Kv1.3 has been reported in many tumors. Kv1.3 promotes tumorigenesis by enhancing cell proliferation while suppressing apoptosis. However, the expression and function of Kv1.3 in osteosarcoma are unknown. In the present study, we detected the e...

Descripción completa

Detalles Bibliográficos
Autores principales: Wu, Jin, Zhong, Daixing, Wu, Xinyu, Sha, Mo, Kang, Liangqi, Ding, Zhenqi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3794831/
https://www.ncbi.nlm.nih.gov/pubmed/24065104
http://dx.doi.org/10.3390/ijms140919245
_version_ 1782287270259195904
author Wu, Jin
Zhong, Daixing
Wu, Xinyu
Sha, Mo
Kang, Liangqi
Ding, Zhenqi
author_facet Wu, Jin
Zhong, Daixing
Wu, Xinyu
Sha, Mo
Kang, Liangqi
Ding, Zhenqi
author_sort Wu, Jin
collection PubMed
description Deregulation of voltage-gated potassium channel subunit Kv1.3 has been reported in many tumors. Kv1.3 promotes tumorigenesis by enhancing cell proliferation while suppressing apoptosis. However, the expression and function of Kv1.3 in osteosarcoma are unknown. In the present study, we detected the expression of Kv1.3 in human osteosarcoma cells and tissues by RT-PCR, Western blot and immunohistochemistry. We further examined cell proliferation and apoptosis in osteosarcoma MG-63 cells and xenografts following knockdown of Kv1.3 by short hairpin RNA (shRNA). We found that Kv1.3 was upregulated in human osteosarcoma. Knockdown of Kv1.3 significantly suppressed cell proliferation and increased apoptosis as demonstrated by enhanced cleavage of poly (ADP-ribose) polymerase (PARP) and the activation of Caspase-3/7. Furthermore, adenovirus delivered shRNA targeting Kv1.3 significantly inhibited the growth of MG-63 xenografts. Taken together, our results suggest that Kv1.3 is a novel molecular target for osterosarcoma therapy.
format Online
Article
Text
id pubmed-3794831
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-37948312013-10-21 Voltage-Gated Potassium Channel Kv1.3 Is Highly Expressed in Human Osteosarcoma and Promotes Osteosarcoma Growth Wu, Jin Zhong, Daixing Wu, Xinyu Sha, Mo Kang, Liangqi Ding, Zhenqi Int J Mol Sci Review Deregulation of voltage-gated potassium channel subunit Kv1.3 has been reported in many tumors. Kv1.3 promotes tumorigenesis by enhancing cell proliferation while suppressing apoptosis. However, the expression and function of Kv1.3 in osteosarcoma are unknown. In the present study, we detected the expression of Kv1.3 in human osteosarcoma cells and tissues by RT-PCR, Western blot and immunohistochemistry. We further examined cell proliferation and apoptosis in osteosarcoma MG-63 cells and xenografts following knockdown of Kv1.3 by short hairpin RNA (shRNA). We found that Kv1.3 was upregulated in human osteosarcoma. Knockdown of Kv1.3 significantly suppressed cell proliferation and increased apoptosis as demonstrated by enhanced cleavage of poly (ADP-ribose) polymerase (PARP) and the activation of Caspase-3/7. Furthermore, adenovirus delivered shRNA targeting Kv1.3 significantly inhibited the growth of MG-63 xenografts. Taken together, our results suggest that Kv1.3 is a novel molecular target for osterosarcoma therapy. MDPI 2013-09-23 /pmc/articles/PMC3794831/ /pubmed/24065104 http://dx.doi.org/10.3390/ijms140919245 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0 This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Review
Wu, Jin
Zhong, Daixing
Wu, Xinyu
Sha, Mo
Kang, Liangqi
Ding, Zhenqi
Voltage-Gated Potassium Channel Kv1.3 Is Highly Expressed in Human Osteosarcoma and Promotes Osteosarcoma Growth
title Voltage-Gated Potassium Channel Kv1.3 Is Highly Expressed in Human Osteosarcoma and Promotes Osteosarcoma Growth
title_full Voltage-Gated Potassium Channel Kv1.3 Is Highly Expressed in Human Osteosarcoma and Promotes Osteosarcoma Growth
title_fullStr Voltage-Gated Potassium Channel Kv1.3 Is Highly Expressed in Human Osteosarcoma and Promotes Osteosarcoma Growth
title_full_unstemmed Voltage-Gated Potassium Channel Kv1.3 Is Highly Expressed in Human Osteosarcoma and Promotes Osteosarcoma Growth
title_short Voltage-Gated Potassium Channel Kv1.3 Is Highly Expressed in Human Osteosarcoma and Promotes Osteosarcoma Growth
title_sort voltage-gated potassium channel kv1.3 is highly expressed in human osteosarcoma and promotes osteosarcoma growth
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3794831/
https://www.ncbi.nlm.nih.gov/pubmed/24065104
http://dx.doi.org/10.3390/ijms140919245
work_keys_str_mv AT wujin voltagegatedpotassiumchannelkv13ishighlyexpressedinhumanosteosarcomaandpromotesosteosarcomagrowth
AT zhongdaixing voltagegatedpotassiumchannelkv13ishighlyexpressedinhumanosteosarcomaandpromotesosteosarcomagrowth
AT wuxinyu voltagegatedpotassiumchannelkv13ishighlyexpressedinhumanosteosarcomaandpromotesosteosarcomagrowth
AT shamo voltagegatedpotassiumchannelkv13ishighlyexpressedinhumanosteosarcomaandpromotesosteosarcomagrowth
AT kangliangqi voltagegatedpotassiumchannelkv13ishighlyexpressedinhumanosteosarcomaandpromotesosteosarcomagrowth
AT dingzhenqi voltagegatedpotassiumchannelkv13ishighlyexpressedinhumanosteosarcomaandpromotesosteosarcomagrowth