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Expression of IL-27 by Tumor Cells in InvasCutaneous and Metastatic Melanomas

Interleukin (IL)-27 is a cytokine of the IL-12 family that displays either immunostimulatory or immunosuppressive functions depending on the context. In various murine tumor models including melanoma models, ectopic expression of IL-27 has been shown to play an anti-tumoral role and to favor tumor r...

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Autores principales: Gonin, Julie, Carlotti, Agnès, Dietrich, Céline, Audebourg, Anne, Radenen-Bussière, Brigitte, Caignard, Anne, Avril, Marie-Françoise, Vacher-Lavenu, Marie-Cécile, Larousserie, Frédérique, Devergne, Odile
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3794994/
https://www.ncbi.nlm.nih.gov/pubmed/24130734
http://dx.doi.org/10.1371/journal.pone.0075694
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author Gonin, Julie
Carlotti, Agnès
Dietrich, Céline
Audebourg, Anne
Radenen-Bussière, Brigitte
Caignard, Anne
Avril, Marie-Françoise
Vacher-Lavenu, Marie-Cécile
Larousserie, Frédérique
Devergne, Odile
author_facet Gonin, Julie
Carlotti, Agnès
Dietrich, Céline
Audebourg, Anne
Radenen-Bussière, Brigitte
Caignard, Anne
Avril, Marie-Françoise
Vacher-Lavenu, Marie-Cécile
Larousserie, Frédérique
Devergne, Odile
author_sort Gonin, Julie
collection PubMed
description Interleukin (IL)-27 is a cytokine of the IL-12 family that displays either immunostimulatory or immunosuppressive functions depending on the context. In various murine tumor models including melanoma models, ectopic expression of IL-27 has been shown to play an anti-tumoral role and to favor tumor regression. In this study, we investigated whether IL-27 might play a role in the development of melanoma in humans. We analyzed the in situ expression of IL-27 in melanocytic lesions (n = 82) representative of different stages of tumor progression. IL-27 expression was not observed in nevus (n = 8) nor in in situ melanoma (n = 9), but was detected in 28/46 (61%) cases of invasive cutaneous melanoma, notably in advanced stages (19/23 cases of stages 3 and 4). In most cases, the main source of IL-27 was tumor cells. Of note, when IL-27 was detected in primary cutaneous melanomas, its expression was maintained in metastatic lesions. These in situ data suggested that the immunosuppressive functions of IL-27 may dominate in human melanoma. Consistent with this hypothesis, we found that IL-27 could induce suppressive molecules such as PD-L1, and to a lesser extent IL-10, in melanoma cells, and that the in situ expression of IL-27 in melanoma correlated with those of PD-L1 and IL-10.
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spelling pubmed-37949942013-10-15 Expression of IL-27 by Tumor Cells in InvasCutaneous and Metastatic Melanomas Gonin, Julie Carlotti, Agnès Dietrich, Céline Audebourg, Anne Radenen-Bussière, Brigitte Caignard, Anne Avril, Marie-Françoise Vacher-Lavenu, Marie-Cécile Larousserie, Frédérique Devergne, Odile PLoS One Research Article Interleukin (IL)-27 is a cytokine of the IL-12 family that displays either immunostimulatory or immunosuppressive functions depending on the context. In various murine tumor models including melanoma models, ectopic expression of IL-27 has been shown to play an anti-tumoral role and to favor tumor regression. In this study, we investigated whether IL-27 might play a role in the development of melanoma in humans. We analyzed the in situ expression of IL-27 in melanocytic lesions (n = 82) representative of different stages of tumor progression. IL-27 expression was not observed in nevus (n = 8) nor in in situ melanoma (n = 9), but was detected in 28/46 (61%) cases of invasive cutaneous melanoma, notably in advanced stages (19/23 cases of stages 3 and 4). In most cases, the main source of IL-27 was tumor cells. Of note, when IL-27 was detected in primary cutaneous melanomas, its expression was maintained in metastatic lesions. These in situ data suggested that the immunosuppressive functions of IL-27 may dominate in human melanoma. Consistent with this hypothesis, we found that IL-27 could induce suppressive molecules such as PD-L1, and to a lesser extent IL-10, in melanoma cells, and that the in situ expression of IL-27 in melanoma correlated with those of PD-L1 and IL-10. Public Library of Science 2013-10-10 /pmc/articles/PMC3794994/ /pubmed/24130734 http://dx.doi.org/10.1371/journal.pone.0075694 Text en © 2013 Gonin et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Gonin, Julie
Carlotti, Agnès
Dietrich, Céline
Audebourg, Anne
Radenen-Bussière, Brigitte
Caignard, Anne
Avril, Marie-Françoise
Vacher-Lavenu, Marie-Cécile
Larousserie, Frédérique
Devergne, Odile
Expression of IL-27 by Tumor Cells in InvasCutaneous and Metastatic Melanomas
title Expression of IL-27 by Tumor Cells in InvasCutaneous and Metastatic Melanomas
title_full Expression of IL-27 by Tumor Cells in InvasCutaneous and Metastatic Melanomas
title_fullStr Expression of IL-27 by Tumor Cells in InvasCutaneous and Metastatic Melanomas
title_full_unstemmed Expression of IL-27 by Tumor Cells in InvasCutaneous and Metastatic Melanomas
title_short Expression of IL-27 by Tumor Cells in InvasCutaneous and Metastatic Melanomas
title_sort expression of il-27 by tumor cells in invascutaneous and metastatic melanomas
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3794994/
https://www.ncbi.nlm.nih.gov/pubmed/24130734
http://dx.doi.org/10.1371/journal.pone.0075694
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