Cargando…
A Modified Semi-Nested Multiplex Malaria PCR (SnM-PCR) for the Identification of the Five Human Plasmodium Species Occurring in Southeast Asia
We have modified an existing semi-nested multiplex polymerase chain reaction (PCR) by adding one Plasmodium knowlesi-specific nested PCR, and validated the latter against laboratory and clinical samples. This new method has the advantage of being relatively affordable in low resource settings while...
| Autores principales: | , , , , , , , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
The American Society of Tropical Medicine and Hygiene
2013
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3795103/ https://www.ncbi.nlm.nih.gov/pubmed/23980132 http://dx.doi.org/10.4269/ajtmh.13-0027 |
| _version_ | 1782287339033198592 |
|---|---|
| author | Van Hong, Nguyen van den Eede, Peter Van Overmeir, Chantal Vythilingham, Indra Rosanas-Urgell, Anna Vinh Thanh, Pham Thang, Ngo Duc Hung, Nguyen Manh Hung, Le Xuan D'Alessandro, Umberto Erhart, Annette |
| author_facet | Van Hong, Nguyen van den Eede, Peter Van Overmeir, Chantal Vythilingham, Indra Rosanas-Urgell, Anna Vinh Thanh, Pham Thang, Ngo Duc Hung, Nguyen Manh Hung, Le Xuan D'Alessandro, Umberto Erhart, Annette |
| author_sort | Van Hong, Nguyen |
| collection | PubMed |
| description | We have modified an existing semi-nested multiplex polymerase chain reaction (PCR) by adding one Plasmodium knowlesi-specific nested PCR, and validated the latter against laboratory and clinical samples. This new method has the advantage of being relatively affordable in low resource settings while identifying the five human Plasmodium species with a three-step PCR. |
| format | Online Article Text |
| id | pubmed-3795103 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2013 |
| publisher | The American Society of Tropical Medicine and Hygiene |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-37951032013-11-04 A Modified Semi-Nested Multiplex Malaria PCR (SnM-PCR) for the Identification of the Five Human Plasmodium Species Occurring in Southeast Asia Van Hong, Nguyen van den Eede, Peter Van Overmeir, Chantal Vythilingham, Indra Rosanas-Urgell, Anna Vinh Thanh, Pham Thang, Ngo Duc Hung, Nguyen Manh Hung, Le Xuan D'Alessandro, Umberto Erhart, Annette Am J Trop Med Hyg Articles We have modified an existing semi-nested multiplex polymerase chain reaction (PCR) by adding one Plasmodium knowlesi-specific nested PCR, and validated the latter against laboratory and clinical samples. This new method has the advantage of being relatively affordable in low resource settings while identifying the five human Plasmodium species with a three-step PCR. The American Society of Tropical Medicine and Hygiene 2013-10-09 /pmc/articles/PMC3795103/ /pubmed/23980132 http://dx.doi.org/10.4269/ajtmh.13-0027 Text en ©The American Society of Tropical Medicine and Hygiene This is an Open Access article distributed under the terms of the American Society of Tropical Medicine and Hygiene's Re-use License which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| spellingShingle | Articles Van Hong, Nguyen van den Eede, Peter Van Overmeir, Chantal Vythilingham, Indra Rosanas-Urgell, Anna Vinh Thanh, Pham Thang, Ngo Duc Hung, Nguyen Manh Hung, Le Xuan D'Alessandro, Umberto Erhart, Annette A Modified Semi-Nested Multiplex Malaria PCR (SnM-PCR) for the Identification of the Five Human Plasmodium Species Occurring in Southeast Asia |
| title | A Modified Semi-Nested Multiplex Malaria PCR (SnM-PCR) for the Identification of the Five Human Plasmodium Species Occurring in Southeast Asia |
| title_full | A Modified Semi-Nested Multiplex Malaria PCR (SnM-PCR) for the Identification of the Five Human Plasmodium Species Occurring in Southeast Asia |
| title_fullStr | A Modified Semi-Nested Multiplex Malaria PCR (SnM-PCR) for the Identification of the Five Human Plasmodium Species Occurring in Southeast Asia |
| title_full_unstemmed | A Modified Semi-Nested Multiplex Malaria PCR (SnM-PCR) for the Identification of the Five Human Plasmodium Species Occurring in Southeast Asia |
| title_short | A Modified Semi-Nested Multiplex Malaria PCR (SnM-PCR) for the Identification of the Five Human Plasmodium Species Occurring in Southeast Asia |
| title_sort | modified semi-nested multiplex malaria pcr (snm-pcr) for the identification of the five human plasmodium species occurring in southeast asia |
| topic | Articles |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3795103/ https://www.ncbi.nlm.nih.gov/pubmed/23980132 http://dx.doi.org/10.4269/ajtmh.13-0027 |
| work_keys_str_mv | AT vanhongnguyen amodifiedseminestedmultiplexmalariapcrsnmpcrfortheidentificationofthefivehumanplasmodiumspeciesoccurringinsoutheastasia AT vandeneedepeter amodifiedseminestedmultiplexmalariapcrsnmpcrfortheidentificationofthefivehumanplasmodiumspeciesoccurringinsoutheastasia AT vanovermeirchantal amodifiedseminestedmultiplexmalariapcrsnmpcrfortheidentificationofthefivehumanplasmodiumspeciesoccurringinsoutheastasia AT vythilinghamindra amodifiedseminestedmultiplexmalariapcrsnmpcrfortheidentificationofthefivehumanplasmodiumspeciesoccurringinsoutheastasia AT rosanasurgellanna amodifiedseminestedmultiplexmalariapcrsnmpcrfortheidentificationofthefivehumanplasmodiumspeciesoccurringinsoutheastasia AT vinhthanhpham amodifiedseminestedmultiplexmalariapcrsnmpcrfortheidentificationofthefivehumanplasmodiumspeciesoccurringinsoutheastasia AT thangngoduc amodifiedseminestedmultiplexmalariapcrsnmpcrfortheidentificationofthefivehumanplasmodiumspeciesoccurringinsoutheastasia AT hungnguyenmanh amodifiedseminestedmultiplexmalariapcrsnmpcrfortheidentificationofthefivehumanplasmodiumspeciesoccurringinsoutheastasia AT hunglexuan amodifiedseminestedmultiplexmalariapcrsnmpcrfortheidentificationofthefivehumanplasmodiumspeciesoccurringinsoutheastasia AT dalessandroumberto amodifiedseminestedmultiplexmalariapcrsnmpcrfortheidentificationofthefivehumanplasmodiumspeciesoccurringinsoutheastasia AT erhartannette amodifiedseminestedmultiplexmalariapcrsnmpcrfortheidentificationofthefivehumanplasmodiumspeciesoccurringinsoutheastasia AT vanhongnguyen modifiedseminestedmultiplexmalariapcrsnmpcrfortheidentificationofthefivehumanplasmodiumspeciesoccurringinsoutheastasia AT vandeneedepeter modifiedseminestedmultiplexmalariapcrsnmpcrfortheidentificationofthefivehumanplasmodiumspeciesoccurringinsoutheastasia AT vanovermeirchantal modifiedseminestedmultiplexmalariapcrsnmpcrfortheidentificationofthefivehumanplasmodiumspeciesoccurringinsoutheastasia AT vythilinghamindra modifiedseminestedmultiplexmalariapcrsnmpcrfortheidentificationofthefivehumanplasmodiumspeciesoccurringinsoutheastasia AT rosanasurgellanna modifiedseminestedmultiplexmalariapcrsnmpcrfortheidentificationofthefivehumanplasmodiumspeciesoccurringinsoutheastasia AT vinhthanhpham modifiedseminestedmultiplexmalariapcrsnmpcrfortheidentificationofthefivehumanplasmodiumspeciesoccurringinsoutheastasia AT thangngoduc modifiedseminestedmultiplexmalariapcrsnmpcrfortheidentificationofthefivehumanplasmodiumspeciesoccurringinsoutheastasia AT hungnguyenmanh modifiedseminestedmultiplexmalariapcrsnmpcrfortheidentificationofthefivehumanplasmodiumspeciesoccurringinsoutheastasia AT hunglexuan modifiedseminestedmultiplexmalariapcrsnmpcrfortheidentificationofthefivehumanplasmodiumspeciesoccurringinsoutheastasia AT dalessandroumberto modifiedseminestedmultiplexmalariapcrsnmpcrfortheidentificationofthefivehumanplasmodiumspeciesoccurringinsoutheastasia AT erhartannette modifiedseminestedmultiplexmalariapcrsnmpcrfortheidentificationofthefivehumanplasmodiumspeciesoccurringinsoutheastasia |