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Pluchea lanceolata protects against Benzo(a) pyrene induced renal toxicity and loss of DNA integrity
Evidence from epidemiological, experimental and clinical trial data indicates that a plant based diet can reduce the risk of chronic diseases and reduces toxic effects. In the present study, we report the antioxidant and anticlastogenic activity of Pluchea lanceolata (PL), an important medicinal pla...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Slovak Toxicology Society SETOX
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3795321/ https://www.ncbi.nlm.nih.gov/pubmed/24170979 http://dx.doi.org/10.2478/intox-2013-0009 |
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author | Jahangir, Tamanna Safhi, Mohammed M. Sultana, Sarwat Ahmad, Sayeed |
author_facet | Jahangir, Tamanna Safhi, Mohammed M. Sultana, Sarwat Ahmad, Sayeed |
author_sort | Jahangir, Tamanna |
collection | PubMed |
description | Evidence from epidemiological, experimental and clinical trial data indicates that a plant based diet can reduce the risk of chronic diseases and reduces toxic effects. In the present study, we report the antioxidant and anticlastogenic activity of Pluchea lanceolata (PL), an important medicinal plant, in both in vitro and in vivo model. Benzo(a)pyrene (B(a)P) administration leads to depletion of renal glutathione and its metabolizing enzymes. Pretreatment with PL (100 and 200 mg /kg b.wt) restored renal glutathione content and its dependent enzymes significantly (p<0.001) with simultaneous increase in catalase(CAT), quinone reductase(QR) in mouse kidney. Prophylactic administration of PL prior to B (a) P administration significantly decreased the malondialdehyde(MDA), H(2)O(2) and xanthineoxidase (XO) levels at a significance of p<0.001, at both the doses. PL extract pretreated groups showed marked inhibition in B(a)P induced micronuclei formation in mouse bone marrow cells with simultaneous restoration of DNA integrity, viz. alkaline unwinding assay and DNA damage shown by gel-electrophoresis. HPTLC confirms the presence of quercetin in plant extract which could be responsible for PL protecting efficacy. In conclusion, the present findings strongly support the antioxidant efficacy of PL, possibly by modulation of antioxidant armory. |
format | Online Article Text |
id | pubmed-3795321 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Slovak Toxicology Society SETOX |
record_format | MEDLINE/PubMed |
spelling | pubmed-37953212013-10-29 Pluchea lanceolata protects against Benzo(a) pyrene induced renal toxicity and loss of DNA integrity Jahangir, Tamanna Safhi, Mohammed M. Sultana, Sarwat Ahmad, Sayeed Interdiscip Toxicol Original Article Evidence from epidemiological, experimental and clinical trial data indicates that a plant based diet can reduce the risk of chronic diseases and reduces toxic effects. In the present study, we report the antioxidant and anticlastogenic activity of Pluchea lanceolata (PL), an important medicinal plant, in both in vitro and in vivo model. Benzo(a)pyrene (B(a)P) administration leads to depletion of renal glutathione and its metabolizing enzymes. Pretreatment with PL (100 and 200 mg /kg b.wt) restored renal glutathione content and its dependent enzymes significantly (p<0.001) with simultaneous increase in catalase(CAT), quinone reductase(QR) in mouse kidney. Prophylactic administration of PL prior to B (a) P administration significantly decreased the malondialdehyde(MDA), H(2)O(2) and xanthineoxidase (XO) levels at a significance of p<0.001, at both the doses. PL extract pretreated groups showed marked inhibition in B(a)P induced micronuclei formation in mouse bone marrow cells with simultaneous restoration of DNA integrity, viz. alkaline unwinding assay and DNA damage shown by gel-electrophoresis. HPTLC confirms the presence of quercetin in plant extract which could be responsible for PL protecting efficacy. In conclusion, the present findings strongly support the antioxidant efficacy of PL, possibly by modulation of antioxidant armory. Slovak Toxicology Society SETOX 2013-03 2013-03 /pmc/articles/PMC3795321/ /pubmed/24170979 http://dx.doi.org/10.2478/intox-2013-0009 Text en Copyright © 2013 Slovak Toxicology Society SETOX http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Jahangir, Tamanna Safhi, Mohammed M. Sultana, Sarwat Ahmad, Sayeed Pluchea lanceolata protects against Benzo(a) pyrene induced renal toxicity and loss of DNA integrity |
title |
Pluchea lanceolata protects against Benzo(a) pyrene induced renal toxicity and loss of DNA integrity |
title_full |
Pluchea lanceolata protects against Benzo(a) pyrene induced renal toxicity and loss of DNA integrity |
title_fullStr |
Pluchea lanceolata protects against Benzo(a) pyrene induced renal toxicity and loss of DNA integrity |
title_full_unstemmed |
Pluchea lanceolata protects against Benzo(a) pyrene induced renal toxicity and loss of DNA integrity |
title_short |
Pluchea lanceolata protects against Benzo(a) pyrene induced renal toxicity and loss of DNA integrity |
title_sort | pluchea lanceolata protects against benzo(a) pyrene induced renal toxicity and loss of dna integrity |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3795321/ https://www.ncbi.nlm.nih.gov/pubmed/24170979 http://dx.doi.org/10.2478/intox-2013-0009 |
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