Real-time investigation of protein unfolding at an air–water interface at the 1 s time scale

Protein unfolding at an air–water interface has been demonstrated such that the X-ray reflectivity can be measured with an acquisition time of 1 s using a recently developed simultaneous multiple-angle–wavelength-dispersive X-ray reflectometer. This has enabled the electron density profile of the ad...

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Autores principales: Yano, Yohko F., Arakawa, Etsuo, Voegeli, Wolfgang, Matsushita, Tadashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2013
Materias:
Diffraction Structural Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3795568/
https://www.ncbi.nlm.nih.gov/pubmed/24121352
http://dx.doi.org/10.1107/S0909049513023741
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author Yano, Yohko F.
Arakawa, Etsuo
Voegeli, Wolfgang
Matsushita, Tadashi
author_facet Yano, Yohko F.
Arakawa, Etsuo
Voegeli, Wolfgang
Matsushita, Tadashi
author_sort Yano, Yohko F.
collection PubMed
description Protein unfolding at an air–water interface has been demonstrated such that the X-ray reflectivity can be measured with an acquisition time of 1 s using a recently developed simultaneous multiple-angle–wavelength-dispersive X-ray reflectometer. This has enabled the electron density profile of the adsorbed protein molecules to be obtained in real time. A globular protein, lysozyme, adsorbed at the air–water interface is found to unfold into a flat shape within 1 s.
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spelling pubmed-37955682013-10-15 Real-time investigation of protein unfolding at an air–water interface at the 1 s time scale Yano, Yohko F. Arakawa, Etsuo Voegeli, Wolfgang Matsushita, Tadashi J Synchrotron Radiat Diffraction Structural Biology Protein unfolding at an air–water interface has been demonstrated such that the X-ray reflectivity can be measured with an acquisition time of 1 s using a recently developed simultaneous multiple-angle–wavelength-dispersive X-ray reflectometer. This has enabled the electron density profile of the adsorbed protein molecules to be obtained in real time. A globular protein, lysozyme, adsorbed at the air–water interface is found to unfold into a flat shape within 1 s. International Union of Crystallography 2013-11-01 2013-10-02 /pmc/articles/PMC3795568/ /pubmed/24121352 http://dx.doi.org/10.1107/S0909049513023741 Text en © Yohko Yano et al. 2013 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Diffraction Structural Biology
Yano, Yohko F.
Arakawa, Etsuo
Voegeli, Wolfgang
Matsushita, Tadashi
Real-time investigation of protein unfolding at an air–water interface at the 1 s time scale
title Real-time investigation of protein unfolding at an air–water interface at the 1 s time scale
title_full Real-time investigation of protein unfolding at an air–water interface at the 1 s time scale
title_fullStr Real-time investigation of protein unfolding at an air–water interface at the 1 s time scale
title_full_unstemmed Real-time investigation of protein unfolding at an air–water interface at the 1 s time scale
title_short Real-time investigation of protein unfolding at an air–water interface at the 1 s time scale
title_sort real-time investigation of protein unfolding at an air–water interface at the 1 s time scale
topic Diffraction Structural Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3795568/
https://www.ncbi.nlm.nih.gov/pubmed/24121352
http://dx.doi.org/10.1107/S0909049513023741
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