Cargando…

The Clinical Impact of Mean Vessel Size and Solidity in Breast Carcinoma Patients

Angiogenesis quantification, through vessel counting or area estimation in the most vascular part of the tumour, has been found to be of prognostic value across a range of carcinomas, breast cancer included. We have applied computer image analysis to quantify vascular properties pertaining to size,...

Descripción completa

Detalles Bibliográficos
Autores principales: Mikalsen, Lars Tore Gyland, Dhakal, Hari Prasad, Bruland, Øyvind S., Naume, Bjørn, Borgen, Elin, Nesland, Jahn M., Olsen, Dag Rune
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3795733/
https://www.ncbi.nlm.nih.gov/pubmed/24146798
http://dx.doi.org/10.1371/journal.pone.0075954
_version_ 1782287424264601600
author Mikalsen, Lars Tore Gyland
Dhakal, Hari Prasad
Bruland, Øyvind S.
Naume, Bjørn
Borgen, Elin
Nesland, Jahn M.
Olsen, Dag Rune
author_facet Mikalsen, Lars Tore Gyland
Dhakal, Hari Prasad
Bruland, Øyvind S.
Naume, Bjørn
Borgen, Elin
Nesland, Jahn M.
Olsen, Dag Rune
author_sort Mikalsen, Lars Tore Gyland
collection PubMed
description Angiogenesis quantification, through vessel counting or area estimation in the most vascular part of the tumour, has been found to be of prognostic value across a range of carcinomas, breast cancer included. We have applied computer image analysis to quantify vascular properties pertaining to size, shape and spatial distributions in photographed fields of CD34 stained sections. Aided by a pilot (98 cases), seven parameters were selected and validated on a separate set from 293 breast cancer patients. Two new prognostic markers were identified through continuous Cox regression with endpoints Breast Cancer Specific Survival and Distant Disease Free Survival: The average size of the vessels as measured by their perimeter (p = 0.003 and 0.004, respectively), and the average complexity of the vessel shapes measured by their solidity (p = 0.004 and 0.004). The Hazard ratios for the corresponding median-dichotomized markers were 2.28 (p = 0.005) and 1.89 (p = 0.016) for the mean perimeter and 1.80 (p = 0.041) and 1.55 (p = 0.095) for the shape complexity. The markers were associated with poor histologic type, high grade, necrosis, HR negativity, inflammation, and p53 expression (vessel size only). Both markers were found to strongly influence the prognostic properties of vascular invasion (VI) and disseminated tumour cells in the bone marrow. The latter being prognostic only in cases with large vessels (p = 0.004 and 0.043) or low complexity (p = 0.018 and 0.024), but not in the small or complex vessel groups (p>0.47). VI was significant in all groups, but showed greater hazard ratios for small and low complexity vessels (6.54–11.2) versus large and high complexity vessels (2.64–3.06). We find that not only the overall amount of produced vasculature in angiogenic hot-spots is of prognostic significance, but also the morphological appearance of the generated vessels, i.e. the size and shape of vessels in the studied hot spots.
format Online
Article
Text
id pubmed-3795733
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-37957332013-10-21 The Clinical Impact of Mean Vessel Size and Solidity in Breast Carcinoma Patients Mikalsen, Lars Tore Gyland Dhakal, Hari Prasad Bruland, Øyvind S. Naume, Bjørn Borgen, Elin Nesland, Jahn M. Olsen, Dag Rune PLoS One Research Article Angiogenesis quantification, through vessel counting or area estimation in the most vascular part of the tumour, has been found to be of prognostic value across a range of carcinomas, breast cancer included. We have applied computer image analysis to quantify vascular properties pertaining to size, shape and spatial distributions in photographed fields of CD34 stained sections. Aided by a pilot (98 cases), seven parameters were selected and validated on a separate set from 293 breast cancer patients. Two new prognostic markers were identified through continuous Cox regression with endpoints Breast Cancer Specific Survival and Distant Disease Free Survival: The average size of the vessels as measured by their perimeter (p = 0.003 and 0.004, respectively), and the average complexity of the vessel shapes measured by their solidity (p = 0.004 and 0.004). The Hazard ratios for the corresponding median-dichotomized markers were 2.28 (p = 0.005) and 1.89 (p = 0.016) for the mean perimeter and 1.80 (p = 0.041) and 1.55 (p = 0.095) for the shape complexity. The markers were associated with poor histologic type, high grade, necrosis, HR negativity, inflammation, and p53 expression (vessel size only). Both markers were found to strongly influence the prognostic properties of vascular invasion (VI) and disseminated tumour cells in the bone marrow. The latter being prognostic only in cases with large vessels (p = 0.004 and 0.043) or low complexity (p = 0.018 and 0.024), but not in the small or complex vessel groups (p>0.47). VI was significant in all groups, but showed greater hazard ratios for small and low complexity vessels (6.54–11.2) versus large and high complexity vessels (2.64–3.06). We find that not only the overall amount of produced vasculature in angiogenic hot-spots is of prognostic significance, but also the morphological appearance of the generated vessels, i.e. the size and shape of vessels in the studied hot spots. Public Library of Science 2013-10-11 /pmc/articles/PMC3795733/ /pubmed/24146798 http://dx.doi.org/10.1371/journal.pone.0075954 Text en © 2013 Mikalsen et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Mikalsen, Lars Tore Gyland
Dhakal, Hari Prasad
Bruland, Øyvind S.
Naume, Bjørn
Borgen, Elin
Nesland, Jahn M.
Olsen, Dag Rune
The Clinical Impact of Mean Vessel Size and Solidity in Breast Carcinoma Patients
title The Clinical Impact of Mean Vessel Size and Solidity in Breast Carcinoma Patients
title_full The Clinical Impact of Mean Vessel Size and Solidity in Breast Carcinoma Patients
title_fullStr The Clinical Impact of Mean Vessel Size and Solidity in Breast Carcinoma Patients
title_full_unstemmed The Clinical Impact of Mean Vessel Size and Solidity in Breast Carcinoma Patients
title_short The Clinical Impact of Mean Vessel Size and Solidity in Breast Carcinoma Patients
title_sort clinical impact of mean vessel size and solidity in breast carcinoma patients
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3795733/
https://www.ncbi.nlm.nih.gov/pubmed/24146798
http://dx.doi.org/10.1371/journal.pone.0075954
work_keys_str_mv AT mikalsenlarstoregyland theclinicalimpactofmeanvesselsizeandsolidityinbreastcarcinomapatients
AT dhakalhariprasad theclinicalimpactofmeanvesselsizeandsolidityinbreastcarcinomapatients
AT brulandøyvinds theclinicalimpactofmeanvesselsizeandsolidityinbreastcarcinomapatients
AT naumebjørn theclinicalimpactofmeanvesselsizeandsolidityinbreastcarcinomapatients
AT borgenelin theclinicalimpactofmeanvesselsizeandsolidityinbreastcarcinomapatients
AT neslandjahnm theclinicalimpactofmeanvesselsizeandsolidityinbreastcarcinomapatients
AT olsendagrune theclinicalimpactofmeanvesselsizeandsolidityinbreastcarcinomapatients
AT mikalsenlarstoregyland clinicalimpactofmeanvesselsizeandsolidityinbreastcarcinomapatients
AT dhakalhariprasad clinicalimpactofmeanvesselsizeandsolidityinbreastcarcinomapatients
AT brulandøyvinds clinicalimpactofmeanvesselsizeandsolidityinbreastcarcinomapatients
AT naumebjørn clinicalimpactofmeanvesselsizeandsolidityinbreastcarcinomapatients
AT borgenelin clinicalimpactofmeanvesselsizeandsolidityinbreastcarcinomapatients
AT neslandjahnm clinicalimpactofmeanvesselsizeandsolidityinbreastcarcinomapatients
AT olsendagrune clinicalimpactofmeanvesselsizeandsolidityinbreastcarcinomapatients