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Inhibition of nm23-H1 gene expression in chronic myelogenous leukemia cells
For solid tumors of a malignant origin, the expression of the nm23-H1 gene is a positive prognostic factor. However, for chronic myeloid leukemia (CML), the prognostic role of nm23-H1 gene expression is unknown. The present study investigated the impact of nm23-H1 gene expression on the proliferatio...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2013
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3796426/ https://www.ncbi.nlm.nih.gov/pubmed/24137469 http://dx.doi.org/10.3892/ol.2013.1500 |
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author | DAI, ZHENSHENG XIAO, WEIZHONG JIN, YUELING |
author_facet | DAI, ZHENSHENG XIAO, WEIZHONG JIN, YUELING |
author_sort | DAI, ZHENSHENG |
collection | PubMed |
description | For solid tumors of a malignant origin, the expression of the nm23-H1 gene is a positive prognostic factor. However, for chronic myeloid leukemia (CML), the prognostic role of nm23-H1 gene expression is unknown. The present study investigated the impact of nm23-H1 gene expression on the proliferation and migration of the CML K562 cell line to elucidate the association between nm23-H1 gene expression and CML cell survival. An RNAi lipo-recombinant plasmid of the nm23-H1 gene (pGCsi-nm23-H1) was constructed and transfected into the K562 cells. RT-PCR and western blotting were used to detect nm23-H1 mRNA and protein expression, respectively. The anchorage-independent growth ability of the transfected cells was observed in soft agar culture and the ability of the K562 cells to migrate was determined using a Transwell assay. Following the successful construction and transfection of the pGCsi-nm23-H1 plasmid into the K562 cells, nm23-H1 mRNA and protein expression levels were significantly lower compared with the control group. The stably-transfected pGCsi-nm23-H1 K562 cells exhibited a markedly increased ability to form colonies and the number and sizes of the colonies were significantly increased compared with those of the control. In vitro, the cells migrated through a Matrigel-coated membrane during incubation for 20 h. The Transwell assay revealed that the quantitative number of pGCsi-nm23-H1 K562 cells that migrated into the lower compartment of the invasion chamber was markedly increased compared with the control. In conclusion, nm23-H1 gene expression may inhibit K562 cell proliferation and migration. nm23-H1 may be a cancer suppressor gene and play a significant role in inhibiting the survival of CML cells. |
format | Online Article Text |
id | pubmed-3796426 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-37964262013-10-17 Inhibition of nm23-H1 gene expression in chronic myelogenous leukemia cells DAI, ZHENSHENG XIAO, WEIZHONG JIN, YUELING Oncol Lett Articles For solid tumors of a malignant origin, the expression of the nm23-H1 gene is a positive prognostic factor. However, for chronic myeloid leukemia (CML), the prognostic role of nm23-H1 gene expression is unknown. The present study investigated the impact of nm23-H1 gene expression on the proliferation and migration of the CML K562 cell line to elucidate the association between nm23-H1 gene expression and CML cell survival. An RNAi lipo-recombinant plasmid of the nm23-H1 gene (pGCsi-nm23-H1) was constructed and transfected into the K562 cells. RT-PCR and western blotting were used to detect nm23-H1 mRNA and protein expression, respectively. The anchorage-independent growth ability of the transfected cells was observed in soft agar culture and the ability of the K562 cells to migrate was determined using a Transwell assay. Following the successful construction and transfection of the pGCsi-nm23-H1 plasmid into the K562 cells, nm23-H1 mRNA and protein expression levels were significantly lower compared with the control group. The stably-transfected pGCsi-nm23-H1 K562 cells exhibited a markedly increased ability to form colonies and the number and sizes of the colonies were significantly increased compared with those of the control. In vitro, the cells migrated through a Matrigel-coated membrane during incubation for 20 h. The Transwell assay revealed that the quantitative number of pGCsi-nm23-H1 K562 cells that migrated into the lower compartment of the invasion chamber was markedly increased compared with the control. In conclusion, nm23-H1 gene expression may inhibit K562 cell proliferation and migration. nm23-H1 may be a cancer suppressor gene and play a significant role in inhibiting the survival of CML cells. D.A. Spandidos 2013-10 2013-07-29 /pmc/articles/PMC3796426/ /pubmed/24137469 http://dx.doi.org/10.3892/ol.2013.1500 Text en Copyright © 2013, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited. |
spellingShingle | Articles DAI, ZHENSHENG XIAO, WEIZHONG JIN, YUELING Inhibition of nm23-H1 gene expression in chronic myelogenous leukemia cells |
title | Inhibition of nm23-H1 gene expression in chronic myelogenous leukemia cells |
title_full | Inhibition of nm23-H1 gene expression in chronic myelogenous leukemia cells |
title_fullStr | Inhibition of nm23-H1 gene expression in chronic myelogenous leukemia cells |
title_full_unstemmed | Inhibition of nm23-H1 gene expression in chronic myelogenous leukemia cells |
title_short | Inhibition of nm23-H1 gene expression in chronic myelogenous leukemia cells |
title_sort | inhibition of nm23-h1 gene expression in chronic myelogenous leukemia cells |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3796426/ https://www.ncbi.nlm.nih.gov/pubmed/24137469 http://dx.doi.org/10.3892/ol.2013.1500 |
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