The ESX-3 Secretion System Is Necessary for Iron and Zinc Homeostasis in Mycobacterium tuberculosis

ESX-3 is one of the five type VII secretion systems encoded by the Mycobacterium tuberculosis genome. We recently showed the essentiality of ESX-3 for M. tuberculosis viability and proposed its involvement in iron and zinc metabolism. In this study we confirmed the role of ESX-3 in iron uptake and i...

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Autores principales: Serafini, Agnese, Pisu, Davide, Palù, Giorgio, Rodriguez, G. Marcela, Manganelli, Riccardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3796483/
https://www.ncbi.nlm.nih.gov/pubmed/24155985
http://dx.doi.org/10.1371/journal.pone.0078351
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author Serafini, Agnese
Pisu, Davide
Palù, Giorgio
Rodriguez, G. Marcela
Manganelli, Riccardo
author_facet Serafini, Agnese
Pisu, Davide
Palù, Giorgio
Rodriguez, G. Marcela
Manganelli, Riccardo
author_sort Serafini, Agnese
collection PubMed
description ESX-3 is one of the five type VII secretion systems encoded by the Mycobacterium tuberculosis genome. We recently showed the essentiality of ESX-3 for M. tuberculosis viability and proposed its involvement in iron and zinc metabolism. In this study we confirmed the role of ESX-3 in iron uptake and its involvement in the adaptation to low zinc environment in M. tuberculosis. Moreover, we unveiled functional differences between the ESX-3 roles in M. tuberculosis and M. smegmatis showing that in the latter ESX-3 is only involved in the adaptation to iron and not to zinc restriction. Finally, we also showed that in M. tuberculosis this secretion system is essential for iron and zinc homeostasis not only in conditions in which the concentrations of these metals are limiting but also in metal sufficient conditions.
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spelling pubmed-37964832013-10-23 The ESX-3 Secretion System Is Necessary for Iron and Zinc Homeostasis in Mycobacterium tuberculosis Serafini, Agnese Pisu, Davide Palù, Giorgio Rodriguez, G. Marcela Manganelli, Riccardo PLoS One Research Article ESX-3 is one of the five type VII secretion systems encoded by the Mycobacterium tuberculosis genome. We recently showed the essentiality of ESX-3 for M. tuberculosis viability and proposed its involvement in iron and zinc metabolism. In this study we confirmed the role of ESX-3 in iron uptake and its involvement in the adaptation to low zinc environment in M. tuberculosis. Moreover, we unveiled functional differences between the ESX-3 roles in M. tuberculosis and M. smegmatis showing that in the latter ESX-3 is only involved in the adaptation to iron and not to zinc restriction. Finally, we also showed that in M. tuberculosis this secretion system is essential for iron and zinc homeostasis not only in conditions in which the concentrations of these metals are limiting but also in metal sufficient conditions. Public Library of Science 2013-10-14 /pmc/articles/PMC3796483/ /pubmed/24155985 http://dx.doi.org/10.1371/journal.pone.0078351 Text en © 2013 Serafini et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Serafini, Agnese
Pisu, Davide
Palù, Giorgio
Rodriguez, G. Marcela
Manganelli, Riccardo
The ESX-3 Secretion System Is Necessary for Iron and Zinc Homeostasis in Mycobacterium tuberculosis
title The ESX-3 Secretion System Is Necessary for Iron and Zinc Homeostasis in Mycobacterium tuberculosis
title_full The ESX-3 Secretion System Is Necessary for Iron and Zinc Homeostasis in Mycobacterium tuberculosis
title_fullStr The ESX-3 Secretion System Is Necessary for Iron and Zinc Homeostasis in Mycobacterium tuberculosis
title_full_unstemmed The ESX-3 Secretion System Is Necessary for Iron and Zinc Homeostasis in Mycobacterium tuberculosis
title_short The ESX-3 Secretion System Is Necessary for Iron and Zinc Homeostasis in Mycobacterium tuberculosis
title_sort esx-3 secretion system is necessary for iron and zinc homeostasis in mycobacterium tuberculosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3796483/
https://www.ncbi.nlm.nih.gov/pubmed/24155985
http://dx.doi.org/10.1371/journal.pone.0078351
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