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Mast Cells Modulate Acute Toxoplasmosis in Murine Models
The role of mast cells (MCs) in Toxoplasma gondii infection is poorly known. Kunming outbred mice were infected intraperitoneally with RH strain T. gondii, either treated with compound 48/80 (C48/80, MC activator) or disodium cromoglycate (DSCG, MC inhibitor). Compared with infected controls, infect...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3797692/ https://www.ncbi.nlm.nih.gov/pubmed/24146978 http://dx.doi.org/10.1371/journal.pone.0077327 |
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author | Huang, Bo Huang, Shiguang Chen, Ying Zheng, Huanqin Shen, Jilong Lun, Zhao-Rong Wang, Yong Kasper, Lloyd H. Lu, Fangli |
author_facet | Huang, Bo Huang, Shiguang Chen, Ying Zheng, Huanqin Shen, Jilong Lun, Zhao-Rong Wang, Yong Kasper, Lloyd H. Lu, Fangli |
author_sort | Huang, Bo |
collection | PubMed |
description | The role of mast cells (MCs) in Toxoplasma gondii infection is poorly known. Kunming outbred mice were infected intraperitoneally with RH strain T. gondii, either treated with compound 48/80 (C48/80, MC activator) or disodium cromoglycate (DSCG, MC inhibitor). Compared with infected controls, infected mice treated with C48/80 exhibited significantly increased inflammation in the liver (P < 0.01), spleen (P < 0.05), and mesentery (P < 0.05) tissues, higher parasite burden in the peritoneal lavage fluids (P < 0.01), and increased levels of mRNA transcripts of T. gondii tachyzoite surface antigen 1 (SAG1) gene in the spleen and liver tissues (P < 0.01), accompanied with significantly increased Th1 cytokine (IFN-γ, IL-12p40, and TNF-α) (P < 0.01) and decreased IL-10 (P < 0.01) mRNA expressions in the liver, and increased IFN-γ (P < 0.01) and IL-12p40 (P < 0.01) but decreased TNF-α (P < 0.01) and IL-4 (P < 0.01) in the spleens of infected mice treated with C48/80 at day 9-10 p.i. Whereas mice treated with DSCG had significantly decreased tissue lesions (P < 0.01), lower parasite burden in the peritoneal lavage fluids (P < 0.01) and decreased SAG1 expressions in the spleen and liver tissues (P < 0.01), accompanied with significantly increased IFN-γ (P < 0.01) and IL-12p40 (P < 0.05) in the liver, and decreased IFN-γ (P < 0.05) and TNF-α (P < 0.01) in the spleens; IL-4 and IL-10 expressions in both the spleen and liver were significantly increased (P < 0.01) in the infected mice treated with DSCG. These findings suggest that mediators associated with the MC activation may play an important role in modulating acute inflammatory pathogenesis and parasite clearance during T. gondii infection in this strain of mice. Thus, MC activation/inhibition mechanisms are potential novel targets for the prevention and control of T. gondii infection. |
format | Online Article Text |
id | pubmed-3797692 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-37976922013-10-21 Mast Cells Modulate Acute Toxoplasmosis in Murine Models Huang, Bo Huang, Shiguang Chen, Ying Zheng, Huanqin Shen, Jilong Lun, Zhao-Rong Wang, Yong Kasper, Lloyd H. Lu, Fangli PLoS One Research Article The role of mast cells (MCs) in Toxoplasma gondii infection is poorly known. Kunming outbred mice were infected intraperitoneally with RH strain T. gondii, either treated with compound 48/80 (C48/80, MC activator) or disodium cromoglycate (DSCG, MC inhibitor). Compared with infected controls, infected mice treated with C48/80 exhibited significantly increased inflammation in the liver (P < 0.01), spleen (P < 0.05), and mesentery (P < 0.05) tissues, higher parasite burden in the peritoneal lavage fluids (P < 0.01), and increased levels of mRNA transcripts of T. gondii tachyzoite surface antigen 1 (SAG1) gene in the spleen and liver tissues (P < 0.01), accompanied with significantly increased Th1 cytokine (IFN-γ, IL-12p40, and TNF-α) (P < 0.01) and decreased IL-10 (P < 0.01) mRNA expressions in the liver, and increased IFN-γ (P < 0.01) and IL-12p40 (P < 0.01) but decreased TNF-α (P < 0.01) and IL-4 (P < 0.01) in the spleens of infected mice treated with C48/80 at day 9-10 p.i. Whereas mice treated with DSCG had significantly decreased tissue lesions (P < 0.01), lower parasite burden in the peritoneal lavage fluids (P < 0.01) and decreased SAG1 expressions in the spleen and liver tissues (P < 0.01), accompanied with significantly increased IFN-γ (P < 0.01) and IL-12p40 (P < 0.05) in the liver, and decreased IFN-γ (P < 0.05) and TNF-α (P < 0.01) in the spleens; IL-4 and IL-10 expressions in both the spleen and liver were significantly increased (P < 0.01) in the infected mice treated with DSCG. These findings suggest that mediators associated with the MC activation may play an important role in modulating acute inflammatory pathogenesis and parasite clearance during T. gondii infection in this strain of mice. Thus, MC activation/inhibition mechanisms are potential novel targets for the prevention and control of T. gondii infection. Public Library of Science 2013-10-16 /pmc/articles/PMC3797692/ /pubmed/24146978 http://dx.doi.org/10.1371/journal.pone.0077327 Text en © 2013 Huang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Huang, Bo Huang, Shiguang Chen, Ying Zheng, Huanqin Shen, Jilong Lun, Zhao-Rong Wang, Yong Kasper, Lloyd H. Lu, Fangli Mast Cells Modulate Acute Toxoplasmosis in Murine Models |
title | Mast Cells Modulate Acute Toxoplasmosis in Murine Models |
title_full | Mast Cells Modulate Acute Toxoplasmosis in Murine Models |
title_fullStr | Mast Cells Modulate Acute Toxoplasmosis in Murine Models |
title_full_unstemmed | Mast Cells Modulate Acute Toxoplasmosis in Murine Models |
title_short | Mast Cells Modulate Acute Toxoplasmosis in Murine Models |
title_sort | mast cells modulate acute toxoplasmosis in murine models |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3797692/ https://www.ncbi.nlm.nih.gov/pubmed/24146978 http://dx.doi.org/10.1371/journal.pone.0077327 |
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