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Nile Blue-Based Nanosized pH Sensors for Simultaneous Far-Red and Near-Infrared Live Bioimaging
[Image: see text] Diblock copolymer vesicles are tagged with pH-responsive Nile Blue-based labels and used as a new type of pH-responsive colorimetric/fluorescent biosensor for far-red and near-infrared imaging of live cells. The diblock copolymer vesicles described herein are based on poly(2-(metha...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2013
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3798108/ https://www.ncbi.nlm.nih.gov/pubmed/24001153 http://dx.doi.org/10.1021/ja407380t |
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author | Madsen, Jeppe Canton, Irene Warren, Nicholas J. Themistou, Efrosyni Blanazs, Adam Ustbas, Burcin Tian, Xiaohe Pearson, Russell Battaglia, Giuseppe Lewis, Andrew L. Armes, Steven P. |
author_facet | Madsen, Jeppe Canton, Irene Warren, Nicholas J. Themistou, Efrosyni Blanazs, Adam Ustbas, Burcin Tian, Xiaohe Pearson, Russell Battaglia, Giuseppe Lewis, Andrew L. Armes, Steven P. |
author_sort | Madsen, Jeppe |
collection | PubMed |
description | [Image: see text] Diblock copolymer vesicles are tagged with pH-responsive Nile Blue-based labels and used as a new type of pH-responsive colorimetric/fluorescent biosensor for far-red and near-infrared imaging of live cells. The diblock copolymer vesicles described herein are based on poly(2-(methacryloyloxy)ethyl phosphorylcholine-block-2-(diisopropylamino)ethyl methacrylate) [PMPC-PDPA]: the biomimetic PMPC block is known to facilitate rapid cell uptake for a wide range of cell lines, while the PDPA block constitutes the pH-responsive component that enables facile vesicle self-assembly in aqueous solution. These biocompatible vesicles can be utilized to detect interstitial hypoxic/acidic regions in a tumor model via a pH-dependent colorimetric shift. In addition, they are also useful for selective intracellular staining of lysosomes and early endosomes via subtle changes in fluorescence emission. Such nanoparticles combine efficient cellular uptake with a pH-responsive Nile Blue dye label to produce a highly versatile dual capability probe. This is in marked contrast to small molecule dyes, which are usually poorly uptaken by cells, frequently exhibit cytotoxicity, and are characterized by intracellular distributions invariably dictated by their hydrophilic/hydrophobic balance. |
format | Online Article Text |
id | pubmed-3798108 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-37981082013-10-18 Nile Blue-Based Nanosized pH Sensors for Simultaneous Far-Red and Near-Infrared Live Bioimaging Madsen, Jeppe Canton, Irene Warren, Nicholas J. Themistou, Efrosyni Blanazs, Adam Ustbas, Burcin Tian, Xiaohe Pearson, Russell Battaglia, Giuseppe Lewis, Andrew L. Armes, Steven P. J Am Chem Soc [Image: see text] Diblock copolymer vesicles are tagged with pH-responsive Nile Blue-based labels and used as a new type of pH-responsive colorimetric/fluorescent biosensor for far-red and near-infrared imaging of live cells. The diblock copolymer vesicles described herein are based on poly(2-(methacryloyloxy)ethyl phosphorylcholine-block-2-(diisopropylamino)ethyl methacrylate) [PMPC-PDPA]: the biomimetic PMPC block is known to facilitate rapid cell uptake for a wide range of cell lines, while the PDPA block constitutes the pH-responsive component that enables facile vesicle self-assembly in aqueous solution. These biocompatible vesicles can be utilized to detect interstitial hypoxic/acidic regions in a tumor model via a pH-dependent colorimetric shift. In addition, they are also useful for selective intracellular staining of lysosomes and early endosomes via subtle changes in fluorescence emission. Such nanoparticles combine efficient cellular uptake with a pH-responsive Nile Blue dye label to produce a highly versatile dual capability probe. This is in marked contrast to small molecule dyes, which are usually poorly uptaken by cells, frequently exhibit cytotoxicity, and are characterized by intracellular distributions invariably dictated by their hydrophilic/hydrophobic balance. American Chemical Society 2013-09-03 2013-10-02 /pmc/articles/PMC3798108/ /pubmed/24001153 http://dx.doi.org/10.1021/ja407380t Text en Copyright © 2013 American Chemical Society Terms of Use CC-BY (http://pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html) |
spellingShingle | Madsen, Jeppe Canton, Irene Warren, Nicholas J. Themistou, Efrosyni Blanazs, Adam Ustbas, Burcin Tian, Xiaohe Pearson, Russell Battaglia, Giuseppe Lewis, Andrew L. Armes, Steven P. Nile Blue-Based Nanosized pH Sensors for Simultaneous Far-Red and Near-Infrared Live Bioimaging |
title | Nile Blue-Based Nanosized pH Sensors for Simultaneous
Far-Red and Near-Infrared Live Bioimaging |
title_full | Nile Blue-Based Nanosized pH Sensors for Simultaneous
Far-Red and Near-Infrared Live Bioimaging |
title_fullStr | Nile Blue-Based Nanosized pH Sensors for Simultaneous
Far-Red and Near-Infrared Live Bioimaging |
title_full_unstemmed | Nile Blue-Based Nanosized pH Sensors for Simultaneous
Far-Red and Near-Infrared Live Bioimaging |
title_short | Nile Blue-Based Nanosized pH Sensors for Simultaneous
Far-Red and Near-Infrared Live Bioimaging |
title_sort | nile blue-based nanosized ph sensors for simultaneous
far-red and near-infrared live bioimaging |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3798108/ https://www.ncbi.nlm.nih.gov/pubmed/24001153 http://dx.doi.org/10.1021/ja407380t |
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